Simultaneous Determination of Flavonoids, Isochlorogenic Acids and Triterpenoids in Ilex hainanensis Using High Performance Liquid Chromatography Coupled with Diode Array and Evaporative Light Scattering Detection
Abstract
:1. Introduction
2. Results and Discussion
2.1. Optimization of Sample Extraction and Chromatographic Conditions
2.2. Validation of HPLC Method
Analytes | Regressive equation | R2 | Linear range (µg/mL) | LOD (µg/mL) | LOQ (µg/mL) |
---|---|---|---|---|---|
1 | y = 4.839x − 1.603 | 0.9994 | 3.36–215.00 | 0.42 | 1.68 |
2 | y =14.732x − 7.012 | 0.9993 | 0.83–212.00 | 0.21 | 0.83 |
3 | y = 12.155x − 15.712 | 0.9994 | 1.69–216.00 | 0.42 | 0.84 |
4 | y = 11.587x − 27.040 | 0.9993 | 3.13–200.00 | 0.39 | 0.78 |
5 | y= 1.603x − 0.447 | 0.9993 | 13.75–330.00 | 6.88 | 13.75 |
6 | y = 1.627x − 0.281 | 0.9991 | 12.56–400.00 | 6.28 | 12.56 |
7 | y = 1.621x − 0.369 | 0.9991 | 13.13–420.00 | 6.56 | 13.13 |
8 | y = 1.762x − 0.782 | 0.9992 | 13.06–313.50 | 6.53 | 13.06 |
Analytes | Precision (RSD, %, n = 6) | Repeatability (RSD, %, n = 3) | Recovery (%, n = 3) | ||||
---|---|---|---|---|---|---|---|
Intra-day | Inter-day | LL | ML | HL | Mean | RSD | |
1 | 0.6 | 1.3 | 2.0 | 0.7 | 2.6 | 98.7 | 2.8 |
2 | 0.8 | 1.2 | 2.4 | 3.4 | 1.9 | 97.6 | 1.3 |
3 | 0.8 | 1.6 | 2.4 | 1.9 | 1.7 | 96.2 | 2.7 |
4 | 1.4 | 1.8 | 2.3 | 3.1 | 1.6 | 100.8 | 0.7 |
5 | 0.8 | 1.0 | 1.1 | 1.2 | 2.4 | 94.6 | 3.5 |
6 | 0.7 | 0.5 | 1.8 | 1.4 | 2.1 | 103.3 | 0.4 |
7 | 0.9 | 1.1 | 2.1 | 2.2 | 2.2 | 98.8 | 4.1 |
8 | 1.5 | 1.6 | 2.4 | 2.6 | 3.2 | 98.0 | 2.8 |
2.3. Quantitative Analysis of Investigated Compounds in Ilex Hainanensis
Samples | Compounds (n = 3) | ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | Tritepenoids (Sum of 5–8) | |
IH1 | 2.30 | 1.34 | 9.95 | 2.45 | 9.28 | 30.69 | 7.47 | 5.64 | 53.08 |
IH2 | 1.14 | 0.26 | 3.41 | 1.22 | 8.75 | 22.05 | 23.32 | 5.08 | 59.20 |
IH3 | 1.13 | 0.51 | 3.18 | 1.46 | 7.72 | 18.23 | 16.07 | 3.95 | 45.97 |
IH4 | 2.52 | 2.12 | 2.56 | 5.91 | 17.62 | 41.17 | 50.57 | 10.99 | 120.35 |
3. Experimental
3.1. Chemical Materials and Reagents
3.2. Sample Preparation
3.3. HPLC-DAD-ELSD Analysis
3.4. Calibration Curves
3.5. Limits of Detection and Quantification
3.6. Precision, Repeatability and Accuracy
4. Conclusions
Acknowledgments
References
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Peng, B.; Qiao, C.-F.; Zhao, J.; Huang, W.-H.; Hu, D.-J.; Liu, H.-G.; Li, S.-P. Simultaneous Determination of Flavonoids, Isochlorogenic Acids and Triterpenoids in Ilex hainanensis Using High Performance Liquid Chromatography Coupled with Diode Array and Evaporative Light Scattering Detection. Molecules 2013, 18, 2934-2941. https://doi.org/10.3390/molecules18032934
Peng B, Qiao C-F, Zhao J, Huang W-H, Hu D-J, Liu H-G, Li S-P. Simultaneous Determination of Flavonoids, Isochlorogenic Acids and Triterpenoids in Ilex hainanensis Using High Performance Liquid Chromatography Coupled with Diode Array and Evaporative Light Scattering Detection. Molecules. 2013; 18(3):2934-2941. https://doi.org/10.3390/molecules18032934
Chicago/Turabian StylePeng, Bo, Chun-Feng Qiao, **g Zhao, Wei-Hua Huang, De-Jun Hu, Hua-Gang Liu, and Shao-** Li. 2013. "Simultaneous Determination of Flavonoids, Isochlorogenic Acids and Triterpenoids in Ilex hainanensis Using High Performance Liquid Chromatography Coupled with Diode Array and Evaporative Light Scattering Detection" Molecules 18, no. 3: 2934-2941. https://doi.org/10.3390/molecules18032934