Regulatory Effect of Astragalus Polysaccharides on Intestinal Intraepithelial γδT Cells of Tumor Bearing Mice
Abstract
:1. Introduction
2. Results and Discussion
2.1. APS Promote Proliferation and Function of Intestinal Intraepithelial γδT Cells in Vitro
2.2. APS Inhibit Tumor Growth in S180 Sarcoma-Bearing Mice
2.3. APS Improve Activity of Intestinal Intraepithelial γδT Cells from Tumor-Bearing Mice
2.4. APS Restore Th1/Th2 Cytokines Balance in Serum of Tumor-Bearing Mice
2.5. Discussion
3. Experimental Section
3.1. Experimental Animals and Cell Lines
3.2. Drug
3.3. Isolation of Intestinal Intraepithelial γδT Cells
3.4. γδT Cells Preparation and Treatment
3.5. Cell Proliferation Assay
3.6. Animal Treatment
3.7. ELISA for Detection of Cytokines Production in Cultured Supernatants and Mice Sera
3.8. Real Time-PCR for Detection of TNF-α, IFN-γ, FasL and Gram B Expression in Intestinal Intraepithelial γδT Cells
3.9. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sun, S.; Zheng, K.; Zhao, H.; Lu, C.; Liu, B.; Yu, C.; Zhang, G.; Bian, Z.; Lu, A.; He, X. Regulatory Effect of Astragalus Polysaccharides on Intestinal Intraepithelial γδT Cells of Tumor Bearing Mice. Molecules 2014, 19, 15224-15236. https://doi.org/10.3390/molecules190915224
Sun S, Zheng K, Zhao H, Lu C, Liu B, Yu C, Zhang G, Bian Z, Lu A, He X. Regulatory Effect of Astragalus Polysaccharides on Intestinal Intraepithelial γδT Cells of Tumor Bearing Mice. Molecules. 2014; 19(9):15224-15236. https://doi.org/10.3390/molecules190915224
Chicago/Turabian StyleSun, Shuyu, Kang Zheng, Hongyan Zhao, Cheng Lu, Biao Liu, Changyuan Yu, Ge Zhang, Zhaoxiang Bian, Ai** Lu, and **aojuan He. 2014. "Regulatory Effect of Astragalus Polysaccharides on Intestinal Intraepithelial γδT Cells of Tumor Bearing Mice" Molecules 19, no. 9: 15224-15236. https://doi.org/10.3390/molecules190915224