Four New Depsides Isolated from Salvia miltiorrhiza and Their Significant Nerve-Protective Activities
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
3.1. General
3.2. Plant Material
3.3. Extraction and Isolation
3.4. Compound Characterization
3.5. HS-SY5Y Cell Culture and Cell Viability Assay
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds 1–12 are available from the authors. |
C | 1 a | 2 b | 3 b | 4 b | ||||
---|---|---|---|---|---|---|---|---|
δH | δC | δH | δC | δH | δC | δH | δC | |
1 | - | 124.9 | - | 122.7 | - | 115.6 | - | 122.5 |
2 | - | 128.0 | - | 124.8 | - | 124.9 | - | 124.9 |
3 | - | 149.6 | - | 147.3 | - | 147.2 | - | 147.1 |
4 | - | 144.8 | - | 143.8 | - | 144.2 | - | 134.9 |
5 | 6.79 d (8.5) | 117.8 | 6.81 d (8.4) | 116.7 | 6.83 d (8.4) | 117.5 | 6.82 d (8.5) | 117.3 |
6 | 7.12 d (8.5) | 122.6 | 7.24 d (8.4) | 121.7 | 7.29 d (8.4) | 120.8 | 7.28 d (8.5) | 121.1 |
7 | 7.56 d (16.0) | 143.7 | 7.53 d (16.0) | 142.9 | 7.52 d (15.8) | 141.8 | 7.57 d (16.0) | 142.7 |
8 | 6.32 d (16.0) | 117.6 | 6.28 d (16.0) | 114.9 | 6.34 d (15.8) | 115.4 | 6.33 d (16.0) | 114.8 |
9 | - | 169.3 | - | 165.9 | - | 165.8 | - | 165.7 |
1′ | - | 130.8 | - | 126.7 | - | 127.7 | - | 126.4 |
2′ | 6.76 br d (1.5) | 117.6 | 6.66 br d (2.0) | 117.2 | 6.66 brd (2.0) | 116.4 | 6.65 br s | 116.6 |
3′ | - | 144.8 | - | 145.6 | - | 144.9 | - | 144.9 |
4′ | - | 150.0 | - | 144.3 | - | 143.9 | - | 144.1 |
5′ | 6.67 d (8.5) | 116.1 | 6.60 d (8.0) | 115.7 | 6.62 d (8.0) | 115.4 | 6.61 d (8.0) | 115.4 |
6′ | 6.61 dd (8.5, 1.5) | 121.8 | 6.36 br d (8.0) | 119.5 | 6.52 dd (8.0, 2.0) | 119.9 | 6.47, dd (8.0, 2.0) | 120.0 |
7′ | 3.07 m; 2.93 m | 38.2 | 2.90, 6.5 d | 36.3 | 2.98 dd (14.0, 4.0) | 36.3 | 2.92 br d (6.5) | 36.2 |
- | - | - | 2.84 m | - | - | - | ||
8′ | 5.00 m | 77.8 | - | 73.1 | - | 73.5 | - | 73.0 |
9′ | - | 177.8 | - | 169.9 | - | 171.2 | - | 169.8 |
1″ | - | 129.3 | - | 131.5 | - | 131.2 | - | 131.2 |
2″ | 6.89 br d (1.5) | 115.0 | 6.68 br d (2.0) | 112.4 | 6.68 br d (2.0) | 112.6 | 6.66 br d (1.5) | 112.5 |
3′’ | - | 145.6 | - | 145.4 | - | 145.1 | - | 144.8 |
4′’ | - | 146.9 | - | 145.4 | - | 145.5 | - | 145.5 |
5′’ | 6.70 d (8.0) | 116.1 | 6.72 d 8.0 | 115.6 | 6.72 d 8.0 | 115.6 | 6.72 d (8.0) | 115.4 |
6′’ | 6.65 m | 119.9 | 6.43 br d (8.0) | 116.7 | 6.55 m | 117.0 | 6.55 m | 116.9 |
7′’ | 5.92 d (11.0) | 88.4 | 5.67 br d (3.0) | 85.8 | 5.65 br d (4.0) | 86.0 | 5.65 brd (4.0) | 85.9 |
8′’ | 4.76 d (11.0) | 54.7 | 4.36 br d (3.0) | 55.4 | 4.43 br d (4.0) | 54.9 | 4.43 br d (4.0) | 55.1 |
9′’ | - | 171.5 | - | 170.1 | - | 170.3 | - | 170.1 |
1′′′ | - | 129.5 | - | 128.1 | - | 126.2 | - | 127.0 |
2′′′ | 6.57 br d (1.5) | 118.1 | 6.55 m | 116.4 | 6.55 m | 116.5 | 6.58 br d (2.0) | 116.4 |
3′′′ | - | 144.9 | - | 144.9 | - | 143.9 | - | 145.4 |
4′′′ | - | 145.0 | - | 144.1 | - | 144.2 | - | 145.1 |
5′′′ | 6.60 d (8.0) | 121.7 | 6.57 d (8.0) | 115.6 | 6.57 d (8.0) | 115.6 | 6.54 d (8.0) | 115.5 |
6′′′ | 6.42 dd (8.0, 1.5) | 122.4 | 6.34 br d (8.0) | 120.0 | 6.32 m | 120.1 | 6.31 dd (8.0, 2.0) | 120.0 |
7′’’ | 2.38 dd (13.5, 6.0) 2.47 dd (13.5, 6.0) | 37.7 | 2.95 dd (14.0, 3.0) 2.80 dd (14.0, 9.0) | 36.3 | 35.8 | 2.86 dd (14.0, 4.5) 2.82 dd (14.0, 8.0) | 35.9 | |
8′′′ | 4.37 t (6.0) | 78.2 | 4.93 dd (9.0, 3.0) | 75.3 | 5.14 dd (8.0, 5.0) | 73.9 | 5.00 dd (8.5, 4.5) | 74.2 |
9′′′ | - | 177.5 | - | 170.7 | - | 169.2 | - | 170.1 |
9′-OCH3 | - | - | 3.60 s | 52.0 | - | - | 3.58 s | 51.0 |
9‴-OCH3 | - | - | - | - | 3.55 s | 51.9 | - | - |
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**, Q.; Hu, X.; Deng, Y.; Hou, J.; Lei, M.; Ji, H.; Zhou, J.; Qu, H.; Wu, W.; Guo, D. Four New Depsides Isolated from Salvia miltiorrhiza and Their Significant Nerve-Protective Activities. Molecules 2018, 23, 3274. https://doi.org/10.3390/molecules23123274
** Q, Hu X, Deng Y, Hou J, Lei M, Ji H, Zhou J, Qu H, Wu W, Guo D. Four New Depsides Isolated from Salvia miltiorrhiza and Their Significant Nerve-Protective Activities. Molecules. 2018; 23(12):3274. https://doi.org/10.3390/molecules23123274
Chicago/Turabian Style**, Qinghao, ** Deng, **jun Hou, Min Lei, Hongjian Ji, **g Zhou, Hua Qu, Wanying Wu, and Dean Guo. 2018. "Four New Depsides Isolated from Salvia miltiorrhiza and Their Significant Nerve-Protective Activities" Molecules 23, no. 12: 3274. https://doi.org/10.3390/molecules23123274