3.1. Effect of TF on Composting Process, pH and GI Values of the Compost
In this study, the whole composting process could be divided into four phases, namely, mesophilic phase (21–40 °C, days 0–1), thermophilic phase (35–65 °C, days 2–17), cooling phase (65–40 °C, days 18–35) and maturation phase (40–25 °C, days 36–43) according to the temperature variation trend of composting. These are consistent with other researcher’s results [
45]. For all TF treatments, the temperature rose rapidly at the beginning and entered the thermophilic phase (>55 °C) on day 2 (
Figure 1a). In the present study, the pile temperature at the CMS7 treatment was kept above 60 °C for a total of 11 days, which was three days less than the other three treatments. In addition, CMS1 treatment remained above 55 °C for 18 days, with 3 days less than that of CMS5. These results were in accordance with the previous studies. It has been shown that premature turning delayed time to achieve high temperatures and turning too late was not helpful for the temperature rising again and the high temperature kee** during composting [
25,
46]. Notably, such high TF aeration surplus as CMS1 treatment not only increased the costs of energy but also decreased the temperature of the composting pile quickly, hindering the thermophilic phase achievement [
47]. However, too low TF (i.e., CMS7 treatment) resulted in lots of anaerobic zone present in the compost system and therefore caused problems of long fermentation time [
48]. An increase in temperature was observed for all treatment after each compost turning as also reported by previous study [
49], thereby indicating that proper TF may increase the oxygen content in the compost, which may result in promoting the activities of microbial organism that can degrade the materials in the compost pile and generating heat [
17].
Moisture content can greatly impact the microbial activity and physicochemical parameters of the compost. As shown in
Figure 1b. the moisture content of each treatment decreased from 65–66% at the beginning to 20.41%, 33.16%, 38.71% and 37.35% on day 43 for CMS1, CMS3, CMS5 and CMS7 treatments, respectively. The composting process may be hindered at higher TF, which might cause higher loss of compost moisture. It has been reported that moisture content is the strongest correlative factor with the succession of bacteria and archaea among numerous other factors such as pH, salinity and nutrients [
50]. Therefore, TF should be appropriately regulated to obtain an efficient composting process.
A key factor which influences gaseous emissions as well as the microbial activities and structure during composting is pH [
33,
51]. In the present study, the overall patterns of pH changes during the composting period were similar for all treatments, which increased dramatically from 6.16–6.48 on day 1 to 8.86–9.17 on day 15 and then gradually increased to 9.23 on day 22 and afterwards reduced slightly (
Figure 1c). The respiration microorganism and of ammonia (NH
3) emission have significant impacts to the pH changes, as have been reported previously [
52,
53]. The lower pH obtained under CMS1 treatment during cooling and mature stage (
p < 0.05), possibly resulted from the release of H
+ ions in the process of nitrification during the transformation of organic nitrogen [
54]. At the end of composting, the final pH of materials was also affected by ammonia volatilization [
6].
The GI always increases with toxic materials degradation in composting pile [
55]. As the composting process progressed, the GI values of CMS1, CMS3 and CMS5 increased, reaching to 91.6%, 93.77% and 124.36% at the end of composting time, respectively (
Figure 1d). The compost is recognized basically and sufficiently mature when the value of GI is above 50% and enough well matured when GI value achieves 80% [
56,
57]. Thus, it can be concluded that the composts of CMS1, CMS3, and CMS5 were stabilized enough at day 43 but the longer time (than 43 days) was required for CMS7 to reach stabilization. TF is commonly thought to be a key factor affecting the rate of composting as well as compost quality [
24]. Previous researchers have suggested that compared to every 4-day and every 7-day turning, every 2-day turning can facilitate faster sterilization and maturity during composting [
22,
58]. In this study, the GI values of the CMS1, CMS3 and CMS5 treatments were 101.52%, 91.64% and 87.24%, respectively, which were significantly higher than that of the CMS7 (46.19%,
p < 0.05).
3.2. Effect of TF on Nitrogen Transformation and NH3 Emission
As shown in
Figure 2a, the concentration of NH
4+-N showed an increasing trend among the four treatments with the temperature reaching its highest on day three. These were primarily attributed to organic nitrogen mineralization and ammonification [
59]. The contents of NH
4+-N in CMS1 and CMS3 group were significantly higher than that in CMS5 and CMS7 on day five suggested higher frequency and premature turning at the beginning of the composting process may promote rapid mineralization and the ammonification during composting (
p < 0.05) [
60]. The difference among the four treatments may be due to the combined effects of the degree of NH
3 emissions, organic nitrogen hydrolysis, and nitrification during the composting [
9]. During the composting process, NH
4+-N might be transformed into NO
3−-N by AOB and AOA, which could then result in reduction of NH
3 emission, and decreasing the loss of organic nitrogen [
61,
62,
63,
64]. However, during thermophilic phase, the excessive NH
3, high temperature and oxygen-deficient of pile can inhibit the proliferation and activity of nitrifying microbial communities [
65]. Comprehensively speaking, losses of NH
4+-N might be the result of the volatilization and nitrification process.
As shown in
Figure 2b, the concentration of NO
3−-N was low at 89.60–96.61 mg/kg initially then it increased to 263.63–483.12 mg/kg on day three during the early composting stages. The NO
3−-N concentration decreased gradually during the cooling phase due to a reduction in the concentration of ammonium [
66]. The NO
3−-N content of CMS1 is significantly lower than that of CMS3, CMS5 or CMS7 during the cooling and maturity period (
p < 0.05). This may because that most of the ammonium in CMS1 used as the substrate for generating NO
3−-N was volatilized in the form of NH
3 due to the high TF, reducing the substrate for ammonia-oxidation reaction for the nitrifying microorganisms [
67]. Or the moisture content in the compost material was greatly reduced for CMS1, which inhibits the activity of nitrifying microorganisms [
50]. In addition, compared with CMS1, CMS3 and CMS7 treatment, the TN loss of CMS5 was decreased by 38.03%, 17.06% and 24.76%, respectively (
Figure 2e). However, because nitrogen metabolism is a complex process, synergistic carbon and nitrogen metabolism, for reducing the nitrogen losses, the activity and quantity of enzymes and the genes, should be further assessed during composting. The relatively appropriate TF may reduce the NH
3 emissions through accelerating the proliferation of AOB and AOA. It was also testified by the changes of NH
4+-N and NO
3-N contents during composting.
As shown in
Figure 2c, NH
3 was detected at day 2. With the temperature and pH increased, NH
3 emissions from all of the treatments rapidly increased and reached their highest values. The NH
3 emission from the CMS1 treatment observed on the third, fifth, ninth and thirteenth day was significantly higher than the other three treatments (
p < 0.05). The high rate of NH
3 emissions for all treatments might be due to quick degradation of organic matter and the fast conversion of NH
4+-N to NH
3 [
13,
34,
68], associated with the increased temperature and pH during the thermophilic stage [
59,
69]. Previous study also suggested the NH
3 volatilization had a positive relationship with the amount of aeration or TF [
70]. The characteristics of NH
3 emissions from this study are in line with previous research [
71,
72]. Overall, physicochemical properties are important factors to influence NH
3 volatilization such as temperature, pH, NH
4+-N concentration, and the microbial community [
12,
73,
74,
75].
As shown in
Figure 2d, the cumulative NH
3 emission profiles indicated that more than 73.67% of NH
3 emissions occurred during the first 23 days in treatments CMS1. However, in the same 23 days, less than 70% of the NH
3 was emitted from the CMS 5, CMS 7 group while more than 70% and less than 73% for CMS3 group. The emission of NH
3 was strongly connected with the temperature of the pile and microorganisms [
74,
76]. NH
3 is mainly important gas causing nitrogen loss during composting. NH
3 emission is mainly affected by temperature, pH, NH
4 +-N concentration, aeration rate, and moisture content [
77]. The differences of NH
3 emissions in all groups were probably due to the interrelationships between pH, temperature, aeration rate and moisture content [
78]. The results showed that the higher TF might lead to the emission of relatively larger amount of accumulative NH
3 emission during the early composting stage, but the detected concentration was similar between different TF groups during the late composting period. Therefore, in general, too high TF results in more ammonia emission during composting.
Figure 2e,f showed the variation of TN and TC content with composting time. TN contents for all treatments presented a similar trend. Compared with CMS1, CMS3 and CMS7 treatments, the TN loss of CMS5 decreased by 38.03%, 17.06% and 24.76%, respectively. Many studies have shown that 16~74% of the initial TN is lost during composting [
75]. The decrease in TN might be due to the large amount of nitrogen loss caused by the NH
3 volatilization, the degradation and mineralization of complex organic compounds [
79]. Nitrogen fixing bacteria might also have contributed to a lesser degree to the increase in TN in the later phase of composting. The TC content of the compost gradually decreased with composting time. This may be attributed to the microorganisms mineralized the organic carbon as a source of energy. The C/N ratio data of compost materials and samples, as shown in the
supplementary Table S6 to give more information about the type of composting waste. The ratio of C/N showed a decreasing trend among the four treatments at the first five days and increased on day 7.
3.3. Effects of TF on Bacterial Community Diversity and Composition during Composting
The five alpha diversity indices (a1–a5) for each treatment at day 1, 5, 15, 29 and 43 during composting are shown in
Figure 3(a1–a5), respectively. The Good’s_coverage index for all samples was over 0.99, indicated that the sequencing depth was enough for this bacterial community analysis. The microbial taxa abundance indices Chao and Observed species were significantly (
p < 0.05) lower in the CMS5 treatment than other treatments at post-thermophilic stage (i.e., day 15), but higher at cooling stage (i.e., day 29). This suggested a significant effect of different TF on bacterial abundance, especially for the post-thermophilic and cooling stage. The microbial richness and evenness indices Shannon and Simpson were also significantly lower in the CMS5 treatment than other treatments at post-thermophilic stage (i.e., day 15). This indicates a selective effect of different TF against bacterial taxa at different composting stages. The Chao and Observed Species indices rose sharply at post-thermophilic stage of composting, which was attributed to the growth of a range of microbiome. Certain bacteria could proliferate during the thermophilic phase, such as amylolytic microorganisms as previous studies reported [
80]. Previous research also suggested higher bacterial abundance and diversity during thermophilic stage of composting for green wastes [
81]. However, research has also shown that microbial activities could be inhibited during thermophilic phase, and the diversity fall may be attributed to the dominance of some microorganism taxa [
82].
The microbial diversity and phylogenetic distribution might have close relationship with the composting process and the quality of compost. Therefore, the composition and succession of bacterial communities at different stages were analyzed. The composition and relative abundance of bacterial communities at phylum and genus levels are shown in
Figure 3(b1,b2), respectively. In total, we detected 27 phyla during composting, with Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes as the top 4 dominant bacteria, which accounted for 92.32–99.82% of the total sequencing reads. This finding agreed with previous studies [
83,
84]. These four bacterial phyla are prevalent throughout the whole composting period and have a strong ability to degradation of organic matter [
85,
86]. Firmicutes often show high enrichment throughout composting because of the ability to form endospores that can help them to keep surviving high temperatures and harsh environment [
85,
87,
88,
89]. In present study, Firmicutes also played a dominant role in the whole composting process, accounting for 44.24–98.20% of the relative abundance. Previous studies also suggested that Proteobacteria are typically the most (or second most) abundant phylum during most aerobic composting [
84]. Interestingly, the relative abundance of Firmicutes in high TF treatments (i.e., CMS1 and CMS3) was significantly lower than that in other treatments, but Proteobacteria and Actinobacteria were significantly higher than that in other treatments during cooling and maturation stages. These results might explain a selective effect of higher TF against bacterial taxa during different composting stages and indicate high aeration conditions could stimulate the growth of aerobic bacteria such as Proteobacteria and Actinobacteria [
90] but inhibit the activity of anaerobic microorganisms in Firmicutes [
91]. Interestingly, the highest abundance of Gemmatimonadetes was found in the CMS5 treatment at the maturation stage. Previous studies also showed that Gemmatimonadetes was probiotics [
92], significantly enriched at the maturation stage of vermicomposting with coconut leaf [
93], and was predominant in soils amended with alkaline treatments [
94]. Thus the beneficial microorganisms may be stimulated by appropriate TF or aeration during aerobic composting [
95].
In total, we detected 569 genera during composting, with Pseudogracilibacillus, Bacillus, Kurthia, Aerococcus, Lactobacillus, Tepidimicrobium, Weissella, Pusillimonas, Sinibacillus and Acinetobacterwere as the top 10 dominant bacteria, which accounted for 27.57–91.19% of the total sequencing reads. These bacterial genera belong to the phyla of Firmicutes, Proteobacteria, Actinobacteria, Gemmatimonadetes and Bacteroidetes, respectively. Pseudogracilibacillus was the most dominant genus accounting for 1.01–83.53% at whole composting stages. The highest relative abundance of Pseudogracilibacillus was found at day 15 (47.27–83.53%), followed by the cooling and the maturation stages (13.52–25.12%). The ecological function of Pseudogracilibacillus during aerobic composting has seldom been reported. Previous study suggested that Pseudogracilibacillus as neutrophilic aerobes could exist in the high-temperature environments and be associated with the nitrogen cycle [
96]. The second dominant genus was Bacillus, accounting for 0.35–50.06% at different composting stages. The relative abundance of Bacillus at the pre-thermophilic stage was 20.01–50.06%, which was significantly higher than that at other stages. This result was in agreement with previous studies, which have reported that the genus Bacillus consists of a large quantity of thermophilic bacteria and can dissimilate and reduce nitrogen compounds [
97]. The relative abundance of Kurthia, Aerococcus, Lactobacillus, Weissella, and Acinetobacter accounted for higher than 1% only at the mesophilic phase (day 1). Pusillimonas has been previously identified as the main dominant bacterial community correlated with the heterotrophic nitrification and denitrification of composting and wastewater [
98,
99]. The abundance of Bacillus, Sinibacillus, Oceanobacillus and Nocardiopsis was significantly higher in CMS1 and CMS3 treatments than other treatments at cooling and mature stages (
p < 0.05). On the contrary, the abundance of Pseudogracilibacillus, Pusillimonas, S0134_terrestrial_group, Limnochordaceae, Alcanivorax and Membranicola was significantly higher in CMS5 and CMS7 treatments at the maturation stage (
p < 0.05). This suggested that these bacterial genera might be sensitive to aeration conditions and could be manipulated by TF of composting materials. Sinibacillus, Limnochordaceae and Oceanobacillus genera have been previously identified as the dominant communities responsible for the proteins transportation-related genus of composting [
33,
100] and wastewater [
101]. Interestingly, as a facultative anaerobe with urease activity, the relative abundance of Pseudograciibacillus decreased with decreasing temperature, which may be attributed to the reduction in ammonia emission [
102,
103].
The significant differences among TF treatments for bacterial communities were identified by LEfSe (
Figure 3(c1–c5),
Supplementary Figure S2(a1–a5), Table S1). Compared to the mesophilic and thermophilic phases, more taxa were significantly affected by TF during cooling phase and maturation phase. The LEfSe of all taxa showed 12, 14, 8, 29 and 55 bacterial taxa had significant differences (LDA > 3,
p < 0.05) among the treatments at mesophilic, pre-thermophilic, post-thermophilic, cooling and maturation phases, respectively. The dominant taxa (LDA > 4,
p < 0.05) were phyla as Firmicutes, Bacteroidetes and genus as
Lactobacillus,
Ureibacillus during mesophilic phase, genus as
Lactobacillus during pre-thermophilic phase, phyla as Actinobacteria and genus as
Corynebacterium_1 during post-thermophilic phase, genus as
Oceanobacillus and
Snibacillus during cooling phase, phyla as Bacteroidetes and genus as
Oceanobacillus,
Georgenia,
Sinibacillus,
Bacillus,
Thermobifida,
Nocardiopsis, Bradymonadales and Membranicola during maturation phase. It must be noted that microbial diversity is related to the physicochemical properties of compost, which change with the composting time [
104]. LEfSe is an accurate and effective method to identify specific microbes (biomarkers) that displayed significant differences in microbial abundance between different treatments [
105]. Among all the different taxas above, phyla as Firmicutes, Bacteroidetes, Actinobacteria and only genus as
Ureibacillus, Lactobacillus, Oceanobacillus, Sinibacillus, Corynebacterium_1, Membranicola also belonged to the top relative abundance10 phylum and top 20 genus, respectively. This indicated that the dominant taxa were significantly affected by different TF. In addition, it should be noticed that the genus
Ureibacillus was significantly affected by different TF during both mesophilic and cooling phase. The genus
Lactobacillus was significantly affected by different TF during both mesophilic and pre-thermophilic phase [
106]. Interestingly,
Sinibacillus, Corynebacterium_1 and
Oceanobacillus were all significantly affected by TF for both post-thermophilic and cooling phase. Meanwhile, previous studies emphasized the importance of low-abundance microorganisms to ecosystem function, such as biochemical processes [
107], community succession [
108] and microbiome function [
109]. Therefore, more attention to these different species caused by different TF during different stages may provide a certain amount of theoretical support for optimizing the composting process.
The keystone taxa for the microbial communities of the four groups during composting were determined using a random forest model (
Supplementary Figure S3(a1,a2)). At the phylum level, nine taxa were the dominant species occupying the top 10 abundance among the top 10 different important phylum for 16S rRNA while
Cyanobacteria as less abundant species was out of the top 10 phylum in abundance. At the genus level, nine taxa were the dominant taxa occupying the top 20 different important genus for 16S rRNA, such as
Pseudogracilibacillus,
Caldicoprobacter, Aerococcus, S0134_terrestrial_group,
Pusillimonas,
Bacillus,
Membranicola, Weissella, Ureibacillus and
Alcanivorax. It was also shown that these classes were common for dominating the composting process [
110].
Pusillimonas was widely distributed in environments and can utilize a variety of fatty acids and urea [
111].
Pseudogracilibacillus,
Ureibacillus and
Alcanivorax are affected by the oxygen concentration and related to nitrogen transformation [
103,
112,
113,
114]. So, it was suggested different TF could affect bacteria involved in N cycle, especially ammonium oxidation. Furthermore, TF can also alter the structure of the bacterial community.
3.4. Effects of TF on Ammonia-Oxidizing Bacteria/Ammonia-Oxidizing Archaeal Diversity and Composition during Composting
AOB and AOA are ubiquitous in various environments and play crucial roles in the nitrogen cycling process [
115,
116,
117]. AOB and AOA also have been found to be common in the composting of various livestock including chicken [
118], cow/cattle [
4,
51,
117], sheep [
119], pig [
120,
121]. Based on the Illumina sequencing data, we obtained an average of 56,022 and 113,491 sequence reads per sample ranging from 15,139–140,517 and 40,925–137,869 reads for AOB and AOA, respectively. The alpha diversity indices of AOB and AOA communities in different TF treatments at day1, 15, 29 and 43 of composting were shown in
Figure 4(a1–b5), respectively. The Good’s_coverage in every sample was over 0.99, suggesting that the sequencing depth was enough for both AOB and AOA community analysis. The Chao and Observed species indices were significantly higher in the CMS1 treatment than in other treatments (
p < 0.05) for AOB at mature stage (i.e., day 43), while lower in the CMS1 treatment than in other treatments (
p < 0.05) for AOA at cooling stage (i.e., day 29). The Shannon and Simpson indices for AOA at the post-thermophilic stage were significantly higher in the CMS5 treatment than the other three treatments, suggesting the inhibition proliferation of AOA communities by high TF treatment through moisture loss or excessive aeration [
120]. As mentioned above, the increase of NO
3−-N concentration in the post-thermophilic composting stage may because that AOA were able to oxidize ammonium under thermophilic conditions and high pH [
71], or caused by high substrate concentration of NH
4+ to accelerate the nitrification microbial growth [
51]. Although it showed relatively higher abundance and diversity at the thermophilic stage of composting in green waste composting [
81], microbial activities could be inhibited during thermophilic phase, and the decline in diversity might be attributed to the dominance of some microbial taxa [
82]. This could be attributed to the differences in temperature, aeration or moisture caused by different TF during the composting stages.
The composition and relative abundance of AOB community at phylum and genus levels are shown in
Figure 4(c1,c2), respectively. In total, we detected 18 phyla during compost, where Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes, were the top 4 dominant bacteria, which accounted for 56.16–99.59% of the total sequencing reads. In this study, Proteobacteria played the most dominant role in the whole composting process, accounting for 25.42–99.55%. Interestingly, the relative abundance of Actinomycetes (ranged from 0.27–30.47%) and Bacteroidetes (ranged from 0–15.72%) at the post-thermophilic period reached the highest of 30.47% and 15.72%, respectively. This may indicate that certain Actinomyces and Bacteroidetestes of AOB may survive at high temperature. The relative abundance of dominant phylum species at different composting stages can be affected by different TF, for instance, the relative abundance of Actinomycetes in the high TF treatment was significantly higher than that in the other groups at post-thermophilic period. In total, we detected 141 genera with roles for AOB during the compost, where
Nitrosospira, Lactobacillus, Nitrosomonas, Weissella and so on were the top 10 dominant bacteria, which accounted for 9.45–99.53% of the total sequencing reads. It was reported that
Nitrosomonas have frequently been investigated in cattle manure or pig slurry amended soils [
122,
123], wastewater treating [
124] and animal manures composting [
4,
125]. For AOB,
Nitrosospira and
Nitrosomonas usually occupied the first or second dominant position in relative abundance during composting [
125]. As previously reported,
Nitrosomonas was typical ammonia-oxidizing bacteria [
126] even dominated all the stages whereas
Nitrosospira dominated the initial of the composting stage and continually decreased in the maturation stage during cow manure composting [
4,
125]. However, in present study,
Nitrosospira played a dominant role in the whole process, especially at the post-thermophilic phase, which reached 8.00%~81.01%; however the proportion of
Nitrosospira sequences increased at higher temperatures [
127]. These findings are in agreement with the previous results that
Nitrosospira can survive at temperatures of up to 75 °C during composting [
128,
129]. Interesting,
Nitrosomonas was almost undetectable during the post-thermophilic phase. These results were in inconsistent with the results that
Nitrosomonas dominated during all the stages of composting [
4,
125]. Moreover, the relative abundance of
Nitrosospira in high TF treatment (i.e., CMS1) was significantly lower than that in the other treatments during post-thermophilic, cooling and maturation stage of the compost, while lower relatively abundant
Nitrosomonas sequences was detected in too high TF (i.e., CMS1) or too low TF (i.e., CMS7) treatment. These findings suggested that proper availability of oxygen, facilitated by turn or aeration, may be an important regulatory factor for AOB in composts [
128].
Microorganisms including bacteria, archaea and fungi played important roles on chicken manure degradation during the composting process [
87,
130,
131]. However, in present study only a total of 3 genera were detected and identified including
Nitrososphaera,
Nitrosopumilus and
Candidatus Nitrosocosmicus, which accounted for 12.18–99.99% of the total sequencing reads (
Figure 4(d1,d2) for AOA at phyhum or genus level). These bacteria all belong to Thaumarchaeota phylum.
Nitrosopumilus and
Nitrososphaera were the dominant AOA species in animal manure composting [
118,
120]. In the present study,
Nitrososphaera played the most dominant role in the whole composting process, accounting for 8.50–99.94%, which was 12.17–47.21% during post-thermophilic phase, and 15.73–99.74% at the cooling and maturation stage. These findings were in accordance with previously studies that
Nitrososphaera belong to Thaumarchaeota phylum dominate in compost and can resist high temperatures [
118,
120].
Nitrosopumilus can be detected in the mesophilic, cooling and maturation phase but not in the post-thermophilic stage suggested Nitrosopumilus may prefer to survive or maintain activity at mesophilic stage [
132]. Interestingly, the relative abundance of
Nitrososphaera increased with the decrease of TF at post-thermophilic stage furthermore the relative abundance of
Nitrososphaera in the high TF treatment was significantly lower than that in the other groups during the post-thermophilic, cooling and maturity periods. These results suggested that the abundance or activity of AOA may be affected by factors such as differing porosity, aeration, or even moisture content [
120,
133,
134].
The significant differences among different TF treatments for the AOB community were identified by LEfSe (
Figure 4(e1–e3),
Supplementary Figure S5(a1–a3), Table S2). The LEfSe of all taxa showed two, eight and four bacterial taxa of AOB were significant different (LDA > 3,
p < 0.05) among the treatments at the post-thermophilic (i.e., day15), cooling (i.e., day 29) and maturation (i.e., day 43) phases, respectively. Specifically, the dominant taxa were genus as
Acinetobacter, Nitrosospira and
Luteimona during post-thermophilic, cooling and maturation phase, respectively. However, LEfSe analysis showed no significant difference among the treatments for AOA community composition. As mentioned above, these results also indicated that the abundance or activity of AOB might be affected by factors such as differing porosity, aeration, or even moisture content [
120,
133,
134].
Furthermore, keystone taxa for the microbial communities of the four groups during composting were determined using a random forest model (
Supplementary Figure S6(a1,a2,b1,b2)). At the phylum level, Seven taxa were the dominant species occupying the top 10 abundance among the top 10 different important genus for AOB such as
Lactobacillus, Nitrosospira, Weissella, Nitrosomonas, Acinetobacter, Escherichia and
Corynebacterium, while
Aerococcus, Muribaculum and
Pseudomonas as less abundant species were out of the top 10 abundance genus. In addition, the four groups were consistent with the dominant flora in abundance among species with different importance at the genus level for AOA.
3.5. Key Environmental Factors Sha** Microbial Communities
RDA (Redundancy analysis) analysis was applied to study the relationship between microbial succession and physicochemical parameters. Using the 16S rRNA and AOB sequencing data, RDA analysis results at the genus level showed that RDA1 and RDA2 jointly explained 61.87% (
Figure 5a) 40.34% (
Figure 5b) and 28.14% (
Figure 5c) of the total variance, respectively. Further analysis revealed that the temperature, NH
4+-N, NH
4+-N, GI, NH
3 emission and NH
3 cumulative emission were the main factors affecting the 16sRNA taxa.
Pusillimonas and
Pseudogracilibacillus were positively correlated to NH
3 emission [
103,
111]. The pH, moisture, NH
3 emission and NH
3 cumulative emission were the main factors affecting the AOB taxa. Interestingly, the moisture content was the only major factor affecting the AOA taxa. This was in agreement with previous study that moisture may affect the amount of dissolved oxygen in the composting [
51]. The temperature, pH, NH
4+-N and NO
3−-N had positive effects on the release of NH
3, while moisture had negative feedback effects on the release of NH
3. These findings suggested that main environmental variables driving the diversity and structure of AOA and AOB communities were different [
135]. In this study the NH
4+-N and NO
3−-N concentration had positive effects on
Nitrososphaera and
Candidatus Nitrosocosmicus. This agreed with previous studies, which reported high substrate concentration of NH
4+-N may promote the AOA growth [
51,
136]. In addition, AOA had a positive relationship with moisture, which supported a previous observation that too high TF accelerated water evaporation and decreased the abundance of ammonia-oxidizing archaea [
120].
Further analysis combined with spearman correlation was used to test the correlation between pH, moisture, ammonia release and out numbers of AOA and AOB (
Supplementary Tables S3–S5). It was found that pH and NH
3 release were significantly correlated with the abundance of AOB and AOA (
p < 0.05). In addition, NH
3 release was significantly positively correlated with pH and negatively correlated with moisture content (
p < 0.001) [
120]. Specifically, NH
3 release was significantly negatively correlated with
Lactobacillus, Weissella, Acinetobacter and positively correlated with
Nitrososphaera as shown in
Supplementary Table S3 during the whole composting (
p < 0.05). While the NH
3 release was significantly negatively correlated with
Lactobacillus, Nitrosomonas, Weissella, Acinetobacter and
Nitrososphaera on day1 and day15 (shown as
Supplementary Table S4), the NH
3 release was significantly negatively correlated with
Nitrosospira and positively correlated with
Acinetobacter on day 29 and day 43 as shown in
Supplementary Table S5 (
p < 0.05). Therefore, in this study AOA and AOB have an important influence on change of NH
3 emission during composting.
Nitrososphaera and
Nitrosospira were significantly negatively correlated with cumulative NH
3 emission (
p < 0.05) during the early (on day 1 and day 15) and late (on day29 and day 43) stage of composting, respectively. In addition, it was found that pH, moisture, structure and abundance of microbial community (AOB/AOA) would all affect NH
3 emission during the composting.
In this study we found that both AOA and AOB have an important influence on the change of NH3 emission during composting. Nitrosospira and Nitrososphaera with high abundance significantly reduced the ammonia emission under turn with suitable frequency during composting of chicken manure. It indicated that we can control the release of NH3 through increasing the abundance of ammonia oxidizing bacteria and archaea. The results can provide more novel theoretical support for efficient utilization of livestock and poultry waste.