Insights on Aggregation of Hen Egg-White Lysozyme from Raman Spectroscopy and MD Simulations
Abstract
:1. Introduction
2. Results
2.1. Protein Unfolding
2.2. Protein Interactions and Aggregation
3. Discussion
3.1. Protein Unfolding
3.2. Protein Interactions and Aggregation
4. Materials and Methods
4.1. Materials
4.2. Lysozyme Sample Preparation and Incubation
4.3. Lysozyme Sample Preparation for Aggregation Inhibition Studies
4.4. Raman Spectroscopy
4.5. Raman Data Analysis: (Baseline Correction, Normalization and Curve Fitting)
4.6. UV-Vis Absorption Spectroscopy
4.7. DLS
4.8. Molecular Dynamics Simulations
4.8.1. Structure of the Hen Egg-White Lysozyme
4.8.2. Molecular Dynamic Simulations
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Chalapathi, D.; Kumar, A.; Behera, P.; Sathi, S.N.; Swaminathan, R.; Narayana, C. Insights on Aggregation of Hen Egg-White Lysozyme from Raman Spectroscopy and MD Simulations. Molecules 2022, 27, 7122. https://doi.org/10.3390/molecules27207122
Chalapathi D, Kumar A, Behera P, Sathi SN, Swaminathan R, Narayana C. Insights on Aggregation of Hen Egg-White Lysozyme from Raman Spectroscopy and MD Simulations. Molecules. 2022; 27(20):7122. https://doi.org/10.3390/molecules27207122
Chicago/Turabian StyleChalapathi, Divya, Amrendra Kumar, Pratik Behera, Shijulal Nelson Sathi, Rajaram Swaminathan, and Chandrabhas Narayana. 2022. "Insights on Aggregation of Hen Egg-White Lysozyme from Raman Spectroscopy and MD Simulations" Molecules 27, no. 20: 7122. https://doi.org/10.3390/molecules27207122