Multicomponent Characterization of the Flower Bud of Panax notoginseng and Its Metabolites in Rat Plasma by Ultra-High Performance Liquid Chromatography/Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry
Abstract
:1. Introduction
2. Results and Discussion
2.1. Development of a UHPLC/IM-QTOF-MS Approach to Separate and Characterize the Multi-Components from PNF by Integrating the Acquisition of HDMSE and DDA Data
2.2. In-Depth Characterization of Ginsenosides from PNF by Analyzing the Negative DDA and HDMSE via the Intelligent UNIFI Workflows
2.2.1. Characterization of PPD-/PPT-Type Ginsenosides
2.2.2. Characterization of Malonyl Ginsenosides
2.3. Identification of the Absorbed Components in Rat Plasma
2.3.1. Prototype Component Identification
2.3.2. Identification of the In Vivo Metabolites
2.3.3. Isomer Verification Based on CCS Prediction
3. Materials and Methods
3.1. Chemicals and Materials
3.2. Sample Preparation
3.3. Animal Experiments and the Preparation of Samples
3.4. UHPLC/IM-QTOF-MS
3.5. Automated Peak Annotation Workflows Facilitated by UNIFITM and Searching an In-House Ginsenoside Library Enabling the Efficient Multicomponent Characterization of PNF
3.6. Identification of the Prototype Compounds and In Vivo Metabolites in Rat Plasma Based on UNIFITM Platform
4. Conclusions
Supplementary Materials
Author Contributions
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Yang, X.; **ong, Y.; Wang, H.; Jiang, M.; Xu, X.; Mi, Y.; Lou, J.; Li, X.; Sun, H.; Zhao, Y.; et al. Multicomponent Characterization of the Flower Bud of Panax notoginseng and Its Metabolites in Rat Plasma by Ultra-High Performance Liquid Chromatography/Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry. Molecules 2022, 27, 9049. https://doi.org/10.3390/molecules27249049
Yang X, **ong Y, Wang H, Jiang M, Xu X, Mi Y, Lou J, Li X, Sun H, Zhao Y, et al. Multicomponent Characterization of the Flower Bud of Panax notoginseng and Its Metabolites in Rat Plasma by Ultra-High Performance Liquid Chromatography/Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry. Molecules. 2022; 27(24):9049. https://doi.org/10.3390/molecules27249049
Chicago/Turabian StyleYang, **aonan, Ying **ong, Hongda Wang, Meiting Jiang, **aoyan Xu, Yueguang Mi, Jia Lou, **aohang Li, He Sun, Yuying Zhao, and et al. 2022. "Multicomponent Characterization of the Flower Bud of Panax notoginseng and Its Metabolites in Rat Plasma by Ultra-High Performance Liquid Chromatography/Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry" Molecules 27, no. 24: 9049. https://doi.org/10.3390/molecules27249049