Catch, Modify and Analyze: Methods of Chemoselective Modification of Cysteine-Containing Peptides
Abstract
:1. Introduction
2. Cysteine-Containing Peptide Enrichment in Solution
2.1. Michael Addition
2.2. Thiol-Disulfide Exchange
Application of Nanoparticles
2.3. Charge Derivatization and Mas Tagging
2.3.1. ICAT Strategy
2.3.2. Mass Tagging
2.3.3. Isotopic Labeling
3. Methods of Thiopeptides Enrichment on Solid Supports
3.1. Michael Addition
3.2. Thiol-Disulfide Exchange
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kowalska, M.; Bąchor, R. Catch, Modify and Analyze: Methods of Chemoselective Modification of Cysteine-Containing Peptides. Molecules 2022, 27, 1601. https://doi.org/10.3390/molecules27051601
Kowalska M, Bąchor R. Catch, Modify and Analyze: Methods of Chemoselective Modification of Cysteine-Containing Peptides. Molecules. 2022; 27(5):1601. https://doi.org/10.3390/molecules27051601
Chicago/Turabian StyleKowalska, Marta, and Remigiusz Bąchor. 2022. "Catch, Modify and Analyze: Methods of Chemoselective Modification of Cysteine-Containing Peptides" Molecules 27, no. 5: 1601. https://doi.org/10.3390/molecules27051601