Analysis of Cell Wall-Related Genes in Organs of Medicago sativa L. under Different Abiotic Stresses
Abstract
:1. Introduction
2. Results and Discussion
2.1. Dirigent and Dirigent-Like Protein Sequences in Medicago truncatula
2.2. Class III Peroxidase Protein Sequences in M. truncatula and M. sativa
2.3. Cell Wall-Related Gene Expression in Alfalfa Roots under Abiotic Stress
2.4. Cell Wall-Related Gene Expression in Alfalfa Leaves under Abiotic Stress
2.5. Cell Wall-Related Gene Expression in Alfalfa Stems under Abiotic Stress
2.6. CesA Genes Expression in Alfalfa under Temperature Stresses: Major Differences in Roots vs. Leaves
3. Materials and Methods
3.1. Identification of Cell Wall-Related Genes in Alfalfa
3.2. Salt, Cold and Heat Stress Treatments of Alfalfa Plants
3.3. RNA Extraction and cDNA Synthesis
3.4. Primer Design, Quantitative Real-Time PCR and Statistical Analysis
4. Conclusions
- (1)
- In the roots, the differences in gene expression are more pronounced as compared to that in the leaves (Figure 5 and Figure 6 and Supplementary Tables S5–S8).
- (2)
- While in the roots primary CesA genes are stable under cold stress, they peak at 72 h in the leaves. Under heat stress, primary CesAs do not show significant changes in expression, neither in roots nor in leaves (Figure 8, panels A and B). On the contrary, heat stress induces a decrease in secondary CesA expression both in roots and in leaves. The decrease is more marked and progressive in the roots (Figure 8, panels C and D).
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Behr, M.; Legay, S.; Hausman, J.-F.; Guerriero, G. Analysis of Cell Wall-Related Genes in Organs of Medicago sativa L. under Different Abiotic Stresses. Int. J. Mol. Sci. 2015, 16, 16104-16124. https://doi.org/10.3390/ijms160716104
Behr M, Legay S, Hausman J-F, Guerriero G. Analysis of Cell Wall-Related Genes in Organs of Medicago sativa L. under Different Abiotic Stresses. International Journal of Molecular Sciences. 2015; 16(7):16104-16124. https://doi.org/10.3390/ijms160716104
Chicago/Turabian StyleBehr, Marc, Sylvain Legay, Jean-Francois Hausman, and Gea Guerriero. 2015. "Analysis of Cell Wall-Related Genes in Organs of Medicago sativa L. under Different Abiotic Stresses" International Journal of Molecular Sciences 16, no. 7: 16104-16124. https://doi.org/10.3390/ijms160716104