Comparative Analysis of the Brassica napus Root and Leaf Transcript Profiling in Response to Drought Stress
Abstract
:1. Introduction
2. Results and Discussion
2.1. Results
2.1.1. Transcriptome Sequencing of Drought Stress
Items | CKR | DSR | CKL | DSL |
---|---|---|---|---|
Total Raw Reads | 11,957,048 | 12,476,345 | 12,338,528 | 12,054,260 |
Reads Containing N | 22 | 29 | 28 | 22 |
Only Adaptors | 115,945 | 81,690 | 84,941 | 68,814 |
Low Quality Reads | 77,646 | 85,765 | 84,565 | 79,619 |
Clean Reads | 11,763,435 | 12,308,861 | 12,168,994 | 11,905,805 |
2.1.2. Differential Gene Expression between the Root and Leaf Libraries
2.1.3. Gene Ontology Function Analysis of DEGs
2.1.4. Pathway Analysis of the DEGs
2.1.5. DEGs Encoding Transcription Factors
2.1.6. Interaction between the Transcription Factors and Hormone-Related Genes
2.1.7. Confirmation of Tag-Mapped Genes by qRT-PCR (Quantitative Real-Time PCR)
2.2. Discussion
2.2.1. Different Regulation Patterns between Root and Leaf Based on Transcriptome Profiles
2.2.2. TFs Functional Protein-Protein Interactions in Hormone Transduction Pathway
2.2.3. Regulation Model for Root and Leaf under Drought Stress
3. Experimental Section
3.1. Plant Materials and Stress Conditions
3.2. RNA Isolation and Illumina Sequencing Results
3.3. Gene Expression Profiling RNA-Seq Analysis
3.4. qRT-PCR Analysis
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Liu, C.; Zhang, X.; Zhang, K.; An, H.; Hu, K.; Wen, J.; Shen, J.; Ma, C.; Yi, B.; Tu, J.; et al. Comparative Analysis of the Brassica napus Root and Leaf Transcript Profiling in Response to Drought Stress. Int. J. Mol. Sci. 2015, 16, 18752-18777. https://doi.org/10.3390/ijms160818752
Liu C, Zhang X, Zhang K, An H, Hu K, Wen J, Shen J, Ma C, Yi B, Tu J, et al. Comparative Analysis of the Brassica napus Root and Leaf Transcript Profiling in Response to Drought Stress. International Journal of Molecular Sciences. 2015; 16(8):18752-18777. https://doi.org/10.3390/ijms160818752
Chicago/Turabian StyleLiu, Chunqing, Xuekun Zhang, Ka Zhang, Hong An, Kaining Hu, **g Wen, **xiong Shen, Chaozhi Ma, Bin Yi, **xing Tu, and et al. 2015. "Comparative Analysis of the Brassica napus Root and Leaf Transcript Profiling in Response to Drought Stress" International Journal of Molecular Sciences 16, no. 8: 18752-18777. https://doi.org/10.3390/ijms160818752