Influence of Genotype on High Glucosinolate Synthesis Lines of Brassica rapa
Abstract
:1. Introduction
2. Results
2.1. Generation of BrYSP DH Lines and GSL Content Profiles
2.2. Resequencing of Parents, HGSL Lines, and LGSL Lines
2.3. SNP Genoty** and InDels
2.4. Identification of GSL Biosynthesis-Specific Recombinant Blocks
Sample ID | No. of Reads | No. of Bases | No. of Clean Reads | Clean Reads (%) | No. of Clean Bases | Clean Bases (%) | De-Duplicated Reads | De- Duplicated Reads (%) | Mapped Reads | Mapped Reads (%) | Ave. Coverage (x) |
---|---|---|---|---|---|---|---|---|---|---|---|
LP08(♀) | 775,036,332 | 78,278,669,532 | 748,357,766 | 96.56 | 75,085,735,554 | 95.92 | 613,028,554 | 81.92 | 555,700,094 | 90.65 | 157.05 |
LP21(♂) | 704,684,562 | 71,173,140,762 | 676,201,118 | 95.96 | 67,699,611,150 | 95.12 | 592,219,743 | 87.58 | 551,893,412 | 93.19 | 156.07 |
BrYSP_DH005 | 135,586,708 | 20,473,592,908 | 132,434,320 | 97.68 | 19,674,335,422 | 96.10 | 121,145,704 | 91.48 | 116,215,959 | 95.93 | 48.48 |
BrYSP_DH009 | 130,566,110 | 19,715,482,610 | 128,070,232 | 98.09 | 19,054,916,880 | 96.65 | 120,327,985 | 93.95 | 115,494,194 | 95.98 | 48.06 |
BrYSP_DH014 | 143,278,512 | 21,635,055,312 | 141,074,216 | 98.46 | 21,128,122,142 | 97.66 | 133,925,200 | 94.93 | 127,330,977 | 95.08 | 53.5 |
BrYSP_DH016 | 134,052,434 | 20,241,917,534 | 132,201,870 | 98.62 | 19,784,969,829 | 97.74 | 123,574,666 | 93.47 | 117,994,498 | 95.48 | 49.38 |
BrYSP_DH017 | 145,561,748 | 21,979,823,948 | 144,029,952 | 98.95 | 21,590,666,966 | 98.23 | 133,547,331 | 92.72 | 126,848,299 | 94.98 | 53.23 |
BrYSP_DH026 | 194,633,904 | 29,389,719,504 | 192,401,316 | 98.85 | 28,832,473,186 | 98.1 | 176,347,873 | 91.66 | 168,647,644 | 95.63 | 70.80 |
BrYSP_DH059 | 126,815,042 | 19,149,071,342 | 125,484,032 | 98.95 | 18,829,119,983 | 98.33 | 117,116,774 | 93.33 | 111,549,893 | 95.25 | 46.89 |
BrYSP_DH061 | 138,437,794 | 20,904,106,894 | 137,010,406 | 98.97 | 20,534,954,754 | 98.23 | 127,865,424 | 93.33 | 122,305,767 | 95.65 | 51.45 |
♀ | HGSL Line | LGSL Lines | ♂ | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Chrom | Start | Stop | Name | Gene ID V1.5 | Gene ID V3.0 | Stages | LP08 | DH 005 | DH 014 | DH 016 | DH 017 | DH 026 | DH 009 | DH 059 | DH 061 | LP21 |
A03 | 21326869 | 21328218 | MYB28.1 | Bra012961 | BraA03g044440.3C | Transcription factors—Aliphatic | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 |
A09 | 3469113 | 3470483 | MYB28.2 | Bra035929 | BraA09g007000.3C | LP08 | LP21 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP08 | LP21 | |
A02 | 25403875 | 25405492 | MYB28.3 | Bra029311 | BraA02g043310.3C | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 | LP08 | LP21 | LP21 | LP21 | |
A03 | 1364309 | 1365719 | MYB29.1 | Bra005949 | BraA03g003070.3C | LP08 | LP08 | LP08 | LP21 | LP21 | LP08 | LP21 | LP21 | LP08 | LP21 | |
A09 | 3356433 | 3357581 | MYB34.1 | Bra035954 | BraA09g006760.3C | Transcription factors—Indolic | LP08 | LP21 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP08 | LP21 |
A03 | 21129279 | 21130669 | MYB34.2 | Bra013000 | BraA03g043850.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 | |
A02 | 25183826 | 25184998 | MYB34.3 | Bra029349 | BraA02g042890.3C | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 | LP08 | LP21 | LP21 | LP21 | |
A02 | 25172906 | 25179858 | MYB34.4 | Bra029350 | BraA02g042880.3C | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 | LP08 | LP21 | LP21 | LP21 | |
A08 | 18248352 | 18249890 | MYB51.2 | Bra016553 | BraA08g028300.3C | LP08 | LP21 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP21 | |
A06 | 6841688 | 6842966 | MYB51.3 | Bra025666 | BraA06g013940.3C | LP08 | LP21 | LP21 | LP08 | LP08 | LP21 | LP08 | LP08 | LP21 | LP21 | |
A07 | 23411313 | 23412768 | MYB122.1 | Bra015939 | BraA07g037950.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | |
A02 | 12327349 | 12329278 | MYB122.2 | Bra008131 | BraA02g022140.3C | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 | LP08 | LP21 | LP08 | LP21 | |
A03 | 18302919 | 18305368 | BCAT-4 | Bra001761 | BraA03g039030.3C | Side chain elongation | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 |
A05 | 18305152 | 18307632 | BCAT-4 | Bra022448 | BraA05g027600.3C | LP08 | LP21 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | |
A06 | 8849237 | 8851230 | BCAT-3 | Bra017964 | BraA06g017190.3C | LP08 | LP21 | LP21 | LP08 | LP08 | LP21 | LP08 | LP08 | LP21 | LP21 | |
A01 | 14814889 | 14817183 | BCAT-3 | Bra029966 | BraA01g023550.3C | LP08 | LP21 | LP21 | LP21 | LP21 | LP21 | LP21 | LP21 | LP21 | LP21 | |
A02 | 2391691 | 2393547 | IPMDH1 | Bra023450 | BraA02g005400.3C | LP08 | LP21 | LP21 | LP21 | LP21 | LP21 | LP08 | LP21 | LP08 | LP21 | |
A03 | 21085392 | 21093219 | MAM1 | Bra013007 | BraA03g043770.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 | |
A03 | 21061112 | 21065885 | MAM3 | Bra013009 | BraA03g043760.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 | |
A03 | 21054537 | 21056963 | MAM3 | Bra013011 | BraA03g043750.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 | |
A02 | 15934744 | 15944413 | MAM1 | Bra018524 | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 | LP08 | LP21 | LP21 | LP21 | ||
A02 | 25115507 | 25119702 | MAM1 | Bra029355 | BraA02g042820.3C | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 | LP08 | LP21 | LP21 | LP21 | |
A02 | 25103449 | 25106649 | MAM3 | Bra029356 | BraA02g042810.3C | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 | LP08 | LP21 | LP21 | LP21 | |
A04 | 5653874 | 5657118 | IPMI LSU1 | Bra032708 | BraA04g008730.3C | LP08 | LP21 | LP21 | LP21 | LP21 | LP21 | LP21 | LP21 | LP21 | LP21 | |
A08 | 5964064 | 5967370 | IPMI LSU1 | Bra040341 | BraA08g006340.3C | LP08 | LP21 | LP21 | LP08 | LP08 | LP21 | LP08 | LP21 | LP21 | LP21 | |
A05 | 1608381 | 1609157 | IPMI SSU2 | Bra004744 | BraA05g003360.3C | LP08 | LP21 | LP21 | LP08 | LP08 | LP21 | LP21 | LP08 | LP21 | LP21 | |
A03 | 12943140 | 12945015 | BAT5 | Bra000760 | BraA03g027810.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 | |
A09 | 17204468 | 17206516 | BAT5 | Bra029434 | BraA09g026220.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | |
A06 | 6007926 | 6010112 | CYP79F1 | Bra026058 | BraA06g012170.3C | Core structure synthesis—Aliphatic | LP08 | LP21 | LP21 | LP08 | LP08 | LP21 | LP08 | LP08 | LP21 | LP21 |
A04 | 5460393 | 5462018 | CYP83A1 | Bra032734 | BraA04g008410.3C | LP08 | LP21 | LP21 | LP21 | LP21 | LP21 | LP21 | LP21 | LP21 | LP21 | |
A02 | 768271 | 770517 | CYP79A2–Aromatic | Bra028764 | BraA02g001710.3C | LP08 | LP21 | LP21 | LP21 | LP21 | LP21 | LP08 | LP21 | LP08 | LP21 | |
A05 | 24811698 | 24812553 | GSTF11 | Bra032010 | BraA05g041750.3C | LP08 | LP21 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP08 | LP21 | |
A07 | 17498418 | 17499341 | GSTU20 | Bra003645 | BraA07g026570.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | |
A01 | 3359620 | 3360837 | GGP1 | Bra011201 | BraA01g007200.3C | LP08 | LP21 | LP21 | LP21 | LP21 | LP21 | LP21 | LP21 | LP08 | LP21 | |
A03 | 27901998 | 27903448 | GGP1 | Bra024068 | BraA03g029390.3C | LP08 | LP08 | LP08 | LP21 | LP21 | LP08 | LP21 | LP21 | LP21 | LP21 | |
A08 | 12833451 | 12834884 | GGP1 | Bra010282 | LP08 | LP21 | LP21 | LP08 | LP08 | LP21 | LP08 | LP21 | LP21 | LP21 | ||
A08 | 12835830 | 12837549 | GGP1 | Bra010283 | BraA08g017720.3C | LP08 | LP21 | LP21 | LP08 | LP08 | LP21 | LP08 | LP21 | LP21 | LP21 | |
A09 | 5949618 | 5952361 | SUR1 | Bra036703 | BraA09g011980.3C | LP08 | LP21 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP08 | LP21 | |
A09 | 25123658 | 25125223 | UGT74B1 | Bra024634 | BraA09g038870.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | |
A01 | 16964878 | 16965828 | ST5b | Bra031476 | BraA01g028650.3C | LP08 | LP21 | LP21 | LP21 | LP21 | LP21 | LP21 | LP21 | LP21 | LP21 | |
A07 | 18479546 | 18480592 | ST5b | Bra003817 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | ||
A07 | 18481748 | 18482782 | ST5b | Bra003818 | BraA07g028360.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | |
A07 | 18002729 | 18003723 | ST5b | Bra003726 | BraA07g027400.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | |
A07 | 23419048 | 23420082 | ST5b | Bra015938 | BraA07g037960.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | |
A07 | 23426595 | 23427635 | ST5b | Bra015936 | BraA07g037980.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | |
A09 | 7726767 | 7727825 | ST5b | Bra027623 | BraA09g012830.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | |
A09 | 9019311 | 9020375 | ST5b | Bra027117 | BraA09g016490.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | |
A09 | 9021641 | 9022747 | ST5b | Bra027118 | BraA09g016500.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | |
A09 | 9977892 | 9978911 | ST5b | Bra027880 | BraA09g017830.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | |
A06 | 6850053 | 6851066 | ST5c | Bra025668 | BraA06g013960.3C | LP08 | LP21 | LP21 | LP08 | LP08 | LP21 | LP08 | LP08 | LP21 | LP21 | |
A01 | 374137 | 375874 | CYP79B2 | Bra011821 | BraA01g000840.3C | Core structure synthesis—Indolic | LP08 | LP21 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP08 | LP21 |
A03 | 31203169 | 31205210 | CYP79B2 | Bra017871 | BraA03g061480.3C | LP08 | LP08 | LP08 | LP21 | LP21 | LP08 | LP21 | LP21 | LP21 | LP21 | |
A08 | 14973444 | 14975600 | CYP79B2 | Bra010644 | BraA08g021670.3C | LP08 | LP21 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP21 | |
A08 | 5058097 | 5059748 | CYP83B1 | Bra034941 | BraA08g007380.3C | LP08 | LP21 | LP21 | LP08 | LP08 | LP21 | LP08 | LP21 | LP21 | LP21 | |
A03 | 7230400 | 7231268 | GSTF9 | Bra022815 | BraA03g016240.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP08 | LP21 | |
A04 | 13901442 | 13902299 | GSTF9 | Bra021673 | BraA04g022220.3C | LP08 | LP21 | LP08 | LP21 | LP21 | LP08 | LP21 | LP21 | LP21 | LP21 | |
A03 | 7232844 | 7233982 | GSTF10 | Bra022816 | BraA03g016250.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP08 | LP21 | |
A02 | 12337180 | 12338199 | ST5a | Bra008132 | BraA02g022150.3C | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 | LP08 | LP21 | LP08 | LP21 | |
A07 | 23427909 | 23428469 | ST5a | Bra015935 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | ||
A08 | 19203810 | 19205763 | FMOGS-OX5 | Bra016787 | BraA08g030720.3C | Side chain modification—Aliphatic | LP08 | LP21 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP21 |
A09 | 8217824 | 8219479 | FMOGS-OX2 | Bra027035 | BraA09g015600.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | |
A02 | 15973741 | 15976278 | AOP2 | Bra018521 | BraA02g027430.3C | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 | LP08 | LP21 | LP21 | LP21 | |
A03 | 13492936 | 13494533 | AOP1 | Bra000847 | BraA03g028760.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 | |
A03 | 13498815 | 13503183 | AOP2 | Bra000848 | BraA03g028770.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 | |
A09 | 1170626 | 1172022 | AOP1 | Bra034182 | BraA09g001250.3C | LP08 | LP21 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP08 | LP21 | |
A09 | 1168503 | 1169786 | AOP1 | Bra034181 | BraA09g001260.3C | LP08 | LP21 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP08 | LP21 | |
A09 | 1165028 | 1166807 | AOP2 | Bra034180 | LP08 | LP21 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP08 | LP21 | ||
A03 | 7768293 | 7769612 | GSL-OH | Bra022920 | BraA03g017350.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP08 | LP21 | |
A04 | 13852551 | 13853809 | GSL-OH | Bra021670 | BraA04g022180.3C | LP08 | LP21 | LP08 | LP21 | LP21 | LP08 | LP21 | LP21 | LP21 | LP21 | |
A04 | 13877397 | 13878646 | GSL-OH | Bra021671 | BraA04g022190.3C | LP08 | LP21 | LP08 | LP21 | LP21 | LP08 | LP21 | LP21 | LP21 | LP21 | |
A02 | 5846392 | 5848174 | CYP81F2 | Bra020459 | BraA02g012540.3C | Side chain modification—Indolic | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 | LP08 | LP21 | LP08 | LP21 |
A03 | 5233924 | 5236233 | CYP81F2 | Bra006830 | BraA03g012390.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP08 | LP21 | |
A03 | 20376817 | 20378290 | APK1 | Bra013120 | BraA03g042630.3C | LP08 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP21 | LP21 | |
A01 | 361733 | 363288 | APK2 | Bra011822 | BraA01g000800.3C | Sulphur supplementation | LP08 | LP21 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP08 | LP21 |
A03 | 31218969 | 31220359 | APK2 | Bra017872 | BraA03g061500.3C | LP08 | LP08 | LP08 | LP21 | LP21 | LP08 | LP21 | LP21 | LP21 | LP21 | |
A08 | 14980248 | 14981406 | APK2 | Bra010645 | BraA08g021680.3C | LP08 | LP21 | LP08 | LP08 | LP08 | LP08 | LP08 | LP21 | LP21 | LP21 |
2.5. Comparative Analysis of GSL Pathway between Individual Genotypes
2.6. GSL Hydrolysis Products
2.7. Nitrile Formation
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. HPLC Analysis for Identification of GSL Content
4.3. Resequencing
4.4. Variant Calling
4.5. Identification of Recombinant Blocks
4.6. Quantification of Glucosinolate Hydrolysis Products
4.7. Measurement of Nitrile Formation
4.8. Statistical Analysis
5. Conclusions
6. Patents
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
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Genotype | Aliphatic | Indolic | Aromatic | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
GRA | GAL | SNG | GNA | GBN | GNL | PRO | GBS | 4-MOGBS | NGBS | GNT | Total μmol g−1 dw | ||
Chiffu | ND | ND | ND | 0.00 e | 0.00 f | 0.00 c | 0.38 ± 0.00 def | 4.00 ± 0.26 a | 1.09 ± 0.04 a | 3.16 ± 0.52 b | 0.14 ± 0.09 de | 8.77 ± 0.69 ef | |
LP08(♀) | 0.11 ± 0.05 b | 0.55 ± 0.05 g | 0.17 ± 0.00 a | 43.40 ± 3.63 b | 0.66 ± 0.06 e | 0.00c | 0.71 ± 0.06 cd | 0.40 ± 0.01 d | 0.31 ± 0.04 c | 0.76 ± 0.06 d | 0.47 ± 0.14 bc | 47.55 ± 3.66 bc | |
LP21(♂) | 0.00b | 0.89 ± 0.10 def | 0.00e | 1.82 ± 0.66 d | 0.00f | 0.27 ± 0.03 a | 0.88 ± 0.09 c | 2.03 ± 0.02 b | 0.63 ± 0.03 b | 3.44 ± 0.24 b | 0.81 ± 0.09 a | 10.76 ± 0.36 e | |
F1 | 0.10 ± 0.01 b | 0.85 ± 0.12 ef | 0.00e | 1.97 ± 0.16 d | 1.68 ± 0.14 d | 0.34 ± 0.03 a | 3.86 ± 0.29 a | 0.59 ± 0.02 c | 0.64 ± 0.03 b | 4.58 ± 0.16 a | 0.47 ± 0.05 bc | 15.07 ± 0.79 d | |
High GSL lines | BrYSP_DH005 | 1.23 ± 0.18 a | 3.47 ± 0.18 a | 0.05 ± 0.01 cde | 35.51 ± 2.84 c | 0.63 ± 0.04 e | 0.10 ± 0.04 b | 0.22 ± 0.03 ef | 0.14 ± 0.00 e | 0.06 ± 0.00 f | 2.03 ± 0.20 c | 0.68 ± 0.34 ab | 44.12 ± 2.86 c |
BrYSP_DH014 | 0.07 ± 0.02 b | 2.16 ± 0.05 b | 0.05 ± 0.01 cde | 45.19 ± 2.69 ab | 7.13 ± 0.62 a | 0.02 ± 0.01 bc | 0.06 ± 0.02 f | 0.11 ± 0.01 e | 0.10 ± 0.01 ef | 0.77 ± 0.04 d | 0.39 ± 0.04 cd | 56.06 ± 3.28 a | |
BrYSP_DH016 | 0.12 ± 0.07 b | 0.43 ± 0.05 g | 0.11 ± 0.07 b | 44.42 ± 1.34 b | 1.68 ± 0.07 d | ND | 0.57 ± 0.37 cde | 0.20 ± 0.01 e | 0.16 ± 0.01 d | 0.76 ± 0.02 d | 0.24 ± 0.03 cde | 48.69 ± 0.95 b | |
BrYSP_DH017 | 0.07 ± 0.01 b | 0.62 ± 0.17 fg | 0.08 ± 0.02 bc | 45.66 ± 1.25 ab | 2.70 ± 0.09 c | 0.10 ± 0.11 b | 0.32 ± 0.03 ef | 0.19 ± 0.01 e | 0.19 ± 0.02 d | 0.50 ± 0.02 def | 0.20 ± 0.01 cde | 50.62 ± 1.51 b | |
BrYSP_DH026 | 0.09 ± 0.02 b | 2.05 ± 0.04 b | 0.07 ± 0.04 bcd | 48.23 ± 1.31 a | 5.39 ± 0.25 b | 0.03 ± 0.02 bc | 0.05 ± 0.00 f | 0.08 ± 0.00 e | 0.11 ± 0.00 e | 0.57 ± 0.05 de | 0.39 ± 0.03 cd | 57.04 ± 1.54 a | |
Low GSL lines | BrYSP_DH009 | 0.04 ± 0.03 b | 1.16 ± 0.10 d | 0.00 ± 0.00 e | 0.25 ± 0.05 d | 0.26 ± 0.07 ef | 0.09 ± 0.03 b | 0.82 ± 0.26 c | 0.12 ± 0.02 e | 0.10 ± 0.01 ef | 0.33 ± 0.03 ef | 0.08 ± 0.03 e | 3.26 ± 0.62 g |
BrYSP_DH059 | 0.06 ± 0.01 b | 1.44 ± 0.19 c | 0.01 ± 0.01 de | 0.32 ± 0.06 d | 0.52 ± 0.08 ef | 0.30 ± 0.03 a | 1.97 ± 0.23 b | 0.10 ± 0.02 e | 0.07 ± 0.01 ef | 0.38 ± 0.03 def | 0.15 ± 0.02 de | 5.31 ± 0.67 fg | |
BrYSP_DH061 | ND | 1.13 ± 0.26 de | 0.01 ± 0.01 de | 2.35 ± 0.81 d | 1.70 ± 0.64 d | 0.00 ± 0.00 c | 0.05 ± 0.01 f | 0.05 ± 0.01 e | 0.01 ± 0.00 g | 0.11 ± 0.02 f | 0.23 ± 0.07 cde | 5.63 ± 1.76 fg | |
ANOVA | Sum of square | 3.852 | 29.573 | 0.091 | 16,645 | 165.68 | 0.525 | 39.6 | 46.36 | 3.601 | 72.88 | 1.669 | 16,984 |
Mean sum of square | 0.350 | 2.688 | 0.008 | 1513.2 | 15.061 | 0.047 | 3.6 | 4.215 | 0.327 | 6.626 | 0.152 | 1544 | |
F value | 68.22 | 103.25 | 9.09 | 348.84 | 132.28 | 22.81 | 80.11 | 471.38 | 494.16 | 129.53 | 7.5 | 286.69 | |
p | **** | **** | **** | **** | **** | **** | **** | **** | **** | **** | **** | **** |
Accessions | BITC | 4-PEITC | 2-PEITC | SFN | Total | Fold |
---|---|---|---|---|---|---|
BrYSP_DH005 | 601.0 ± 16.8 | 47.7 ± 1.3 | 40.8 ± 0.8 | 20.24 ± 1.33 | 709.8 ± 16.7 | 5.1 |
BrYSP_DH014 | 778.8 ± 32.9 | 64.5 ± 3.9 | 25.0 ± 1.2 | 2.03 ± 0.15 | 870.3 ± 37.6 | 6.3 |
BrYSP_DH016 | 425.1 ± 10.0 | 100.1 ± 2.5 | 18.5 ± 0.7 | 2.90 ± 0.13 | 546.6 ± 12.5 | 3.9 |
BrYSP_DH017 | 281.7 ± 15.3 | 120.7 ± 5.3 | 12.7 ± 0.8 | 2.35 ± 0.21 | 417.5 ± 21.5 | 3.0 |
BrYSP_DH026 | 653.0 ± 20.8 | 94.0 ± 2.3 | 34.5 ± 0.5 | 2.39 ± 0.15 | 783.9 ± 23.5 | 5.6 |
pak choi© | 108.9 ± 2.7 | 21.6 ± 0.6 | 8.1 ± 0.2 | 0.57 ± 0.04 | 139.2 ± 3.6 | Control |
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Soundararajan, P.; Park, S.-G.; Won, S.Y.; Moon, M.-S.; Park, H.W.; Ku, K.-M.; Kim, J.S. Influence of Genotype on High Glucosinolate Synthesis Lines of Brassica rapa. Int. J. Mol. Sci. 2021, 22, 7301. https://doi.org/10.3390/ijms22147301
Soundararajan P, Park S-G, Won SY, Moon M-S, Park HW, Ku K-M, Kim JS. Influence of Genotype on High Glucosinolate Synthesis Lines of Brassica rapa. International Journal of Molecular Sciences. 2021; 22(14):7301. https://doi.org/10.3390/ijms22147301
Chicago/Turabian StyleSoundararajan, Prabhakaran, Sin-Gi Park, So Youn Won, Mi-Sun Moon, Hyun Woo Park, Kang-Mo Ku, and Jung Sun Kim. 2021. "Influence of Genotype on High Glucosinolate Synthesis Lines of Brassica rapa" International Journal of Molecular Sciences 22, no. 14: 7301. https://doi.org/10.3390/ijms22147301