SH3-Binding Glutamic Acid Rich-Deficiency Augments Apoptosis in Neonatal Rat Cardiomyocytes
Abstract
:1. Introduction
2. Results
2.1. SH3BGR Is Confined to Striated Muscle and Upregulated in Cardiac Hypertrophy
2.2. SH3BGR Induces Cellular Hypertrophy in NRVCMs
2.3. SH3BGR Regulates RhoA–SRF Signaling in NRVCMs
2.4. SH3BGR Knockdown Affects NRVCM-Viability and Induces Apoptosis via HIPPO Signaling
3. Discussion
4. Materials and Methods
4.1. Cloning of SH3BGR Vectors
4.2. Antibodies
4.3. Isolation of NRVCMs
4.4. Co-Localization Analysis of SH3BGR with α-Actinin
4.5. Immunofluorescence Microscopy for Cell Size Measurement
4.6. MTT Assay for Cell Viability
4.7. RNA Isolation and qRT-PCR
4.8. Protein Preparation and Immunoblotting
4.9. Human Heart Samples
4.10. SRF Luciferase Assay
4.11. RhoA Inhibitor Usage
4.12. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
CHD | Congenital heart disease |
DS | Down’s syndrome |
SH3BGR | SH3-binding glutamic acid rich |
Nppa | Natriuretic peptide A |
Nppb | Natriuretic peptide B |
NRVCM | Neonatal rat ventricular cardiomyocytes |
RhoA | Ras Homolog family member A |
SRF | Serum Response Factor |
YAP | Yes associated protein |
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Deshpande, A.; Borlepawar, A.; Rosskopf, A.; Frank, D.; Frey, N.; Rangrez, A.Y. SH3-Binding Glutamic Acid Rich-Deficiency Augments Apoptosis in Neonatal Rat Cardiomyocytes. Int. J. Mol. Sci. 2021, 22, 11042. https://doi.org/10.3390/ijms222011042
Deshpande A, Borlepawar A, Rosskopf A, Frank D, Frey N, Rangrez AY. SH3-Binding Glutamic Acid Rich-Deficiency Augments Apoptosis in Neonatal Rat Cardiomyocytes. International Journal of Molecular Sciences. 2021; 22(20):11042. https://doi.org/10.3390/ijms222011042
Chicago/Turabian StyleDeshpande, Anushka, Ankush Borlepawar, Alexandra Rosskopf, Derk Frank, Norbert Frey, and Ashraf Yusuf Rangrez. 2021. "SH3-Binding Glutamic Acid Rich-Deficiency Augments Apoptosis in Neonatal Rat Cardiomyocytes" International Journal of Molecular Sciences 22, no. 20: 11042. https://doi.org/10.3390/ijms222011042