Analysis of the Promoter Regions of gga-miR-31 and Its Regulation by RA and C-jun in Chicken
Abstract
:1. Introduction
2. Results
2.1. gga-miR-31 Promoter Core Region Analysis
2.2. Bioinformatics Analysis of the Promoter Region of gga-miR-31
2.3. gga-miR-31 Is Transcriptionally Activated by RA(retinoic acid) and Competes with the Promoter of Stra8 to Bind RA
2.4. Effect of Transcription Factor C-jun Binding Sites on gga-miR-31 Promoter Activity
2.5. Transcription Factor C-jun Inhibited the Transcription of gga-miR-31, and Combined with RA, Affected the Promoter Activity of gga-miR-31
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Materials
4.3. Bioinformatics Analysis of Promoter
4.4. Promoter Amplification and Vector Construction
4.8. Data Analysis
4.9. Data Availability
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Shao, X.; Gong, W.; Wang, Q.; Wang, P.; Shi, T.; Mahmut, A.; Qin, J.; Yao, Y.; Yan, W.; Chen, D.; et al. Atrophic skeletal muscle fibre-derived small extracellular vesicle miR-690 inhibits satellite cell differentiation during ageing. J. Cachexia Sarcopenia Muscle 2022, 13, 3163–3180. [Google Scholar] [CrossRef]
- Walker, W.H. Regulation of mammalian spermatogenesis by miRNAs. Semin. Cell Dev. Biol. 2022, 121, 24–31. [Google Scholar] [CrossRef] [PubMed]
- Yoshida, K.; Yokoi, A.; Yamamoto, Y.; Kajiyama, H. ChrXq27.3 miRNA cluster functions in cancer development. J. Exp. Clin. Cancer Res. 2021, 40, 112. [Google Scholar] [CrossRef] [PubMed]
- Zhu, B.; Zhong, W.; Cao, X.; Pan, G.; Xu, M.; Zheng, J.; Chen, H.; Feng, X.; Luo, C.; Lu, C.; et al. Loss of miR-31-5p drives hematopoietic stem cell malignant transformation and restoration eliminates leukemia stem cells in mice. Sci. Transl. Med. 2022, 14, eabh2548. [Google Scholar] [CrossRef]
- Yu, F.; Liang, M.; Huang, Y.; Wu, W.; Zheng, B.; Chen, C. Hypoxic tumor-derived exosomal miR-31-5p promotes lung adenocarcinoma metastasis by negatively regulating SATB2-reversed EMT and activating MEK/ERK signaling. J. Exp. Clin. Cancer Res. 2021, 40, 179. [Google Scholar] [CrossRef]
- Fong, L.Y.; Taccioli, C.; Palamarchuk, A.; Tagliazucchi, G.M.; **g, R.; Smalley, K.J.; Fan, S.; Altemus, J.; Fiehn, O.; Huebner, K.; et al. Abrogation of esophageal carcinoma development in miR-31 knockout rats. Proc. Natl. Acad. Sci. USA 2020, 117, 6075–6085. [Google Scholar] [CrossRef] [Green Version]
- Yong, Z.; Eva, S.; Deepak, S. Serum response factor regulates a muscle-specific microRNA that targets Hand2 during cardiogenesis. Nature 2005, 436, 214–220. [Google Scholar]
- Yin, M.; Lü, M.; Yao, G.; Tian, H.; Lian, J.; Liu, L.; Liang, M.; Wang, Y.; Sun, F. Transactivation of microRNA-383 by steroidogenic factor-1 promotes estradiol release from mouse ovarian granulosa cells by targeting RBMS1. Mol. Endocrinol. 2012, 26, 1129–1143. [Google Scholar] [CrossRef] [Green Version]
- Jiang, X.; Xue, M.; Fu, Z.; Ji, C.; Guo, X.; Zhu, L.; Xu, L.; Pang, L.; Xu, M.; Qu, H. Insight into the effects of adipose tissue inflammation factors on miR-378 expression and the underlying mechanism. Cell. Physiol. Biochem. 2014, 33, 1778–1788. [Google Scholar] [CrossRef]
- Zhao, Y.; He, J.; Yang, L.; Luo, Q.; Liu, Z. Histone Deacetylase-3 Modification of MicroRNA-31 Promotes Cell Proliferation and Aerobic Glycolysis in Breast Cancer and Is Predictive of Poor Prognosis. J. Breast Cancer 2018, 21, 112–123. [Google Scholar] [CrossRef]
- Yang, K.; Ren, J.; Li, X.; Wang, Z.; Xue, L.; Cui, S.; Sang, W.; Xu, T.; Zhang, J.; Yu, J.; et al. Prevention of aortic dissection and aneurysm via an ALDH2-mediated switch in vascular smooth muscle cell phenotype. Eur. Heart J. 2020, 41, 2442–2453. [Google Scholar] [CrossRef]
- Liu, Z. Cloning of chicken stra8 gene promoter and its related inducers screening. Master’s Thesis, Yangzhou University, Yangzhou, China, 2014. [Google Scholar]
- **, J. Mechanism Analysis of lncRNA-BMP4 Regulating Chicken PGCs Formation. Master’s Thesis, Yangzhou University, Yangzhou, China, 2020. [Google Scholar]
- Wang, Y.; Zuo, Q.; Bi, Y.; Zhang, W.; **, J.; Zhang, L.; Zhang, Y.-N.; Li, B. miR-31 Regulates Spermatogonial Stem Cells Meiosis via Targeting Stra8. J. Cell Biochem. 2017, 118, 4844–4853. [Google Scholar] [CrossRef]
- Zhang, Z. Study of the Critical Genes in Male Germ Cell differentiation from Chicken Embryonic Stem Cell. Ph.D. Thesis, Yangzhou University, Yangzhou, China, 2014. [Google Scholar]
- Ripamonti, A.; Provasi, E.; Lorenzo, M.; De Simone, M.; Ranzani, V.; Vangelisti, S.; Curti, S.; Bonnal, R.J.P.; Pignataro, L.; Torretta, S.; et al. Repression of miR-31 by BCL6 stabilizes the helper function of human follicular helper T cells. Proc. Natl. Acad. Sci. USA 2017, 114, 12797–12802. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Tang, Y.; Liu, B.; Li, N.; Cong, W.; **ao, J. Regulatory mechanisms of miRNA-31-5p in excess retinoic acid-inducedabnormal C2C12 cell proliferation. J. Dalian Med. Univ. 2016, 38, 6–11. [Google Scholar]
- Zhang, X.; Liu, M.; Jia, M. Regulation of gene expresssion during spermatogenesis at transcriptional level. Hereditas 2011, 33, 1300–1307. [Google Scholar]
- Liu, C.; Yu, P.; Ren, Z.; Yao, F.; Wang, L.; Hu, G.; Li, P.; Zhao, Q. Rif1 Regulates Self-Renewal and Impedes Mesendodermal Differentiation of Mouse Embryonic Stem Cells. Stem Cell Rev. Rep. 2023, 19, 1540–1553. [Google Scholar] [CrossRef] [PubMed]
- Avellino, R.; Havermans, M.; Erpelinck, C.; Sanders, M.A.; Hoogenboezem, R.; van de Werken, H.J.G.; Rombouts, E.; van Lom, K.; van Strien, P.M.H.; Gebhard, C.; et al. An autonomous CEBPA enhancer specific for myeloid-lineage priming and neutrophilic differentiation. Blood 2016, 127, 2991–3003. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Chieffi, P.; Angelini, F.; Pierantoni, R. Detection of c-Myc, c-Fos, and c-Jun-like products in the lizard (Podarcis s. sicula) testis. Ann. N. Y. Acad. Sci. 1998, 839, 561–563. [Google Scholar] [CrossRef]
- Xu, S.; Yuan, S.; Wang, J. Effect of c-jun on apoptosis of rat leydig cells in vitro. J. CUN (Nat. Sci. Ed.) 2006, 15, 197–202. [Google Scholar]
- Xu, S.; Wang, J.; Yuan, S. Effect of c-jun on basic and hCG-induced testosterone secretion of ret leyig cells in vitro. J. CUN (Nat. Sci. Ed.) 2005, 14, 241–244. [Google Scholar]
- Okumura, K.; Hosoe, Y.; Nakajima, N. c-Jun and Sp1 family are critical for retinoic acid induction of the lamin A/C retinoic acid-responsive element. Biochem. Biophys. Res. Commun. 2004, 320, 487–492. [Google Scholar] [CrossRef] [PubMed]
- Zhang, M.; Xu, P.; Sun, X.; Zhang, C.; Shi, X.; Li, J.; Jiang, J.; Chen, C.; Zhang, Y.; Chen, G.; et al. JUN promotes chicken female differentiation by inhibiting Smad2. Cytotechnology 2021, 73, 101–113. [Google Scholar] [CrossRef] [PubMed]
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Wang, Y.; Kong, R.; **e, K.; Hu, C.; Zhao, Z.; Wu, Y.; Zuo, Q.; Li, B.; Zhang, Y. Analysis of the Promoter Regions of gga-miR-31 and Its Regulation by RA and C-jun in Chicken. Int. J. Mol. Sci. 2023, 24, 12516. https://doi.org/10.3390/ijms241512516
Wang Y, Kong R, **e K, Hu C, Zhao Z, Wu Y, Zuo Q, Li B, Zhang Y. Analysis of the Promoter Regions of gga-miR-31 and Its Regulation by RA and C-jun in Chicken. International Journal of Molecular Sciences. 2023; 24(15):12516. https://doi.org/10.3390/ijms241512516
Chicago/Turabian StyleWang, Yingjie, Ruihong Kong, Ke **e, Cai Hu, Zongyi Zhao, Yuhui Wu, Qisheng Zuo, Bichun Li, and Yani Zhang. 2023. "Analysis of the Promoter Regions of gga-miR-31 and Its Regulation by RA and C-jun in Chicken" International Journal of Molecular Sciences 24, no. 15: 12516. https://doi.org/10.3390/ijms241512516