Molecular Regulatory Network of Anthocyanin Accumulation in Black Radish Skin as Revealed by Transcriptome and Metabonome Analysis
Abstract
:1. Introduction
2. Results
2.1. Identification of Flavonoids in the Root Skin of Two Radish RILs
2.2. Transcriptome Analysis of Root Skin of Two Radish RILs
2.3. Co-Expression Network Analysis Identified Flavonoid-Related DEGs
2.4. TFs Involved in Flavonoid Synthesis
2.5. Candidate Hub Genes Related to Flavonoid Synthesis
2.6. Results of the Transcriptome and Metabolome Comprehensive Analyses
2.7. Verification of RNA-Seq Results by qRT-PCR
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Flavonoid Identification and Quantification
4.3. RNA-Seq and Differential Expression Analysis
4.4. Co-Expression Network Analysis for the Construction of Modules
4.5. Comprehensive Analysis of the Transcriptome and Metabolome
4.6. QRT-PCR
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Meta ID | Metabolite Name | Ion Abundance | ||||
---|---|---|---|---|---|---|
b1 | b2 | b3 | b4 | w4 | ||
CWJP002202 | Cyanidin-3-O-sophorotrioside-5-O-malonylglucoside | 1.83 × 104 | 2.70 × 105 | 4.45 × 105 | 1.99 × 105 | 9.00 |
CWJP002409 | Cyanidin-3-O-p-coumaroyl-sophoroside-5-O-malonyl-glucoside | 5.43 × 104 | 2.58 × 105 | 1.75 × 105 | 1.88 × 105 | 9.00 |
CWJP002544 | Cyanidin-3-O-feruloyl-sophoroside-5-O-malonylglucoside | 1.39 × 105 | 1.67 × 106 | 1.31 × 106 | 1.35 × 106 | 9.00 |
CWJP002576 | Cyanidin-3-O-di-caffeoyl-diglucoside-5-O-malonyl-glucoside | 4.74 × 103 | 2.93 × 104 | 4.06 × 104 | 1.77 × 104 | 6.70 × 102 |
CWJP002621 | Cyanidin-3-O-p-coumaroyl-feruloyl-sophoroside-5-O-malonyl-glucoside | 1.62 × 104 | 3.25 × 104 | 4.13 × 104 | 3.57 × 104 | 7.88 × 102 |
CWJP002795 | Cyanidin-3-O-p-coumaroyl-caffeoylsophorotrioside-7-O-malonyl-glucoside | 6.55 × 103 | 5.84 × 104 | 1.11 × 105 | 3.96 × 104 | 9.00 |
CWJP002833 | Cyanidin-di-O-malonyl-glucoside-O-malonyl-diglucoside | 9.51 × 103 | 5.50 × 104 | 7.36 × 104 | 4.41 × 104 | 9.00 |
CWJP002934 | Cyanidin-3-O-p-coumaroyl-caffeoyl-sophoroside-5-O-succinoyl-glucoside | 8.28 × 103 | 5.17 × 104 | 5.02 × 104 | 5.17 × 104 | 9.00 |
CWJP002964 | Cyanidin-3-O-sinapoyl-5-O-dimalonylglucoside-glucoside | 6.52 × 103 | 1.49 × 104 | 6.72 × 103 | 8.07 × 103 | 3.62 × 102 |
pmb0550 | Cyanidin 3-O-glucoside (Kuromanin) | 2.51 × 106 | 1.90 × 106 | 2.25 × 106 | 3.69 × 106 | 1.31 × 104 |
pmb0557 | Cyanidin 3-O-(3″,6″-dimalonylglucoside) | 9.20 × 104 | 1.97 × 105 | 2.17 × 105 | 9.00 | 9.00 |
pmb2962 | Pelargonidin 3-O-(6″-acetylglucoside) | 1.12 × 103 | 9.26 × 104 | 4.99 × 104 | 1.02 × 106 | 9.00 |
pme1777 | Cyanidin 3,5-O-diglucoside (Cyanin) | 2.22 × 103 | 1.67 × 103 | 1.56 × 103 | 5.70 × 103 | 3.43 × 102 |
pmf0203 | Peonidin 3-O-glucoside | 1.03 × 106 | 9.70 × 105 | 1.99 × 105 | 4.91 × 105 | 4.37 × 105 |
pmf0617 | Peonidin 3-O-sophoroside 5-O-glucoside | 4.15 × 104 | 5.44 × 104 | 1.43 × 104 | 6.76 × 104 | 9.00 |
pmf0619 | Peonidin 3-O-feruloylsophoroside-5-glucoside. | 1.07 × 104 | 5.58 × 104 | 5.96 × 103 | 1.36 × 104 | 9.00 |
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Zhang, J.; Zhang, Z.-X.; Wen, B.-Y.; Jiang, Y.-J.; He, X.; Bai, R.; Zhang, X.-L.; Chai, W.-C.; Xu, X.-Y.; Xu, J.; et al. Molecular Regulatory Network of Anthocyanin Accumulation in Black Radish Skin as Revealed by Transcriptome and Metabonome Analysis. Int. J. Mol. Sci. 2023, 24, 13663. https://doi.org/10.3390/ijms241713663
Zhang J, Zhang Z-X, Wen B-Y, Jiang Y-J, He X, Bai R, Zhang X-L, Chai W-C, Xu X-Y, Xu J, et al. Molecular Regulatory Network of Anthocyanin Accumulation in Black Radish Skin as Revealed by Transcriptome and Metabonome Analysis. International Journal of Molecular Sciences. 2023; 24(17):13663. https://doi.org/10.3390/ijms241713663
Chicago/Turabian StyleZhang, **g, Zi-Xuan Zhang, Bo-Yue Wen, Ya-Jie Jiang, **a He, Rui Bai, **n-Ling Zhang, Wen-Chen Chai, **ao-Yong Xu, ** Xu, and et al. 2023. "Molecular Regulatory Network of Anthocyanin Accumulation in Black Radish Skin as Revealed by Transcriptome and Metabonome Analysis" International Journal of Molecular Sciences 24, no. 17: 13663. https://doi.org/10.3390/ijms241713663