A Gold Nanoclusters Film Supported on Polydopamine for Fluorescent Sensing of Free Bilirubin
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Instrumentations
2.2. Preparation of the Gold Nanoclusters Film by Polydopamine Adhesion
2.3. Fluorescence Measurements
3. Results and Discussion
3.1. Characterization of PDA/AuNCs Film
3.2. PDA/AuNCs Film for Fluorescent Sensing of fBR
3.3. Determination of fBR in Human Blood Serum
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
- Fevery, J. Bilirubin in clinical practice: A review. Liver Int. 2008, 28, 592–605. [Google Scholar] [CrossRef]
- Westwood, A. The analysis of bilirubin in serum. Ann. Clin. Biochem. 1991, 28, 119–130. [Google Scholar] [CrossRef] [PubMed]
- Hansen, T. Mechanisms of bilirubin toxicity: Clinical implications. Clin. Perinatol. 2002, 29, 765–778. [Google Scholar] [CrossRef]
- Chen, S.; Lu, W.; Yueh, M.; Rettenmeier, E.; Liu, M.; Auwerx, J.; Yu, R.; Evans, R.; Wang, K.; Karind, M. Intestinal NCoR1, a regulator of epithelial cell maturation, controls neonatal hyperbilirubinemia. Proc. Natl. Acad. Sci. USA 2017, 114, E1432–E1440. [Google Scholar] [PubMed] [Green Version]
- Shapiro, S. Bilirubin toxicity in the develo** nervous system. Pediatr. Neurol. 2003, 29, 410–421. [Google Scholar] [CrossRef]
- Ahlfors, C.; Wennberg, R.; Ostrow, J.; Tiribelli, C. Unbound (free) bilirubin: Improving the paradigm for evaluating neonatal jaundice. Clin. Chem. 2009, 55, 1288–1299. [Google Scholar] [PubMed]
- Ameri, M.; Schnaars, H.; Sibley, J.; Honor, D. Comparison of the vanadate oxidase method with the diazo method for serum bilirubin determination in dog, monkey, and rat. J. Vet. Diagn. Investig. 2011, 123, 120–123. [Google Scholar]
- Batra, B.; Lata, S.; Rana, J.; Pundir, C.S. Construction of an amperometric bilirubin biosensor based on covalent immobilization of bilirubin oxidase onto zirconia coated silica nanoparticles/chitosan hybrid film. Biosens. Bioelectron. 2013, 44, 64–69. [Google Scholar] [PubMed]
- Noh, H.; Won, M.; Shim, Y. Selective nonenzymatic bilirubin detection in blood samples using a nafion/mn–cu sensor. Biosens. Bioelectron. 2014, 61, 554–561. [Google Scholar] [CrossRef]
- Martelanc, M.; Žiberna, L.; Passamonti, S.; Franko, M. Direct determination of fBR in serum at sub-nanomolar levels. Anal. Chim. Acta 2014, 809, 174–182. [Google Scholar] [CrossRef] [PubMed]
- Moein, M.M.; Jabbar, D.; Colmsjö, A.; Abdelrehim, M. A needle extraction utilizing a molecularly imprinted-sol-gel xerogel for on-line microextraction of the lung cancer biomarker bilirubin from plasma and urine samples. J. Chromatogr. A 2014, 1366, 15–23. [Google Scholar] [CrossRef]
- Sun, H.; Nie, Z.; Fung, Y.S. Determination of fBR and its binding capacity by HSA using a microfluidic chip-capillary electrophoresis device with a multi-segment circular-ferrofluid-driven micromixing injection. Electrophoresis 2010, 31, 3061–3069. [Google Scholar] [CrossRef] [PubMed]
- Li, H.X.; **, R.; Kong, D.S.; Zhao, X.; Liu, F.M.; Yan, X.; Lin, Y.H.; Lu, G.Y. Switchable fluorescence immunoassay using gold nanoclusters anchored cobalt oxyhydroxide composite for sensitive detection of imidacloprid. Sens. Actuators B Chem. 2019, 283, 207–214. [Google Scholar] [CrossRef]
- **ao, W.; Han, G.; Chen, Z. Ratiometric fluorescent sensing of copper ion based on a pyrene schiff base langmuir-blodgett film. Sens. Lett. 2015, 13, 501–505. [Google Scholar] [CrossRef]
- **ao, W.; Chen, Z. Fluorescent iron (III) determination based on salicylaldehyde functionalized bimodal mesoporous silica. J. Nanosci. Nanotechnol. 2016, 16, 12666–12670. [Google Scholar] [CrossRef]
- Kohashi, K.; Date, Y.; Morita, M.; Tsuruta, Y. Fluorescence reaction of bilirubin with zinc ion in dimethyl sulfoxide and its application to assay of total bilirubin in serum. Anal. Chim. Acta 1998, 365, 177–182. [Google Scholar] [CrossRef]
- Wabaidur, S.M.; Eldesoky, G.E.; Alothman, Z.A. The fluorescence quenching of Ru(bipy)32+: An application for the determination of bilirubin in biological samples. Luminescence 2018, 33, 625–629. [Google Scholar] [CrossRef] [PubMed]
- Kamruzzaman, M.; Alam, A.M.; Hak Lee, S.; Ho Kim, Y.; Kim, G.M.; Hyub Oh, S. Spectrofluorimetric quantification of bilirubin using yttrium–norfloxacin complex as a fluorescence probe in serum samples. J. Lumin. 2012, 132, 3053–3057. [Google Scholar] [CrossRef]
- Jayasree, M.; Aparna, R.S.; Anjana, R.R.; Devi, J.S.A.; John, N.; Abha, K.; Manikandan, A.; George, S. Fluorescence turn on detection of bilirubin using Fe (III) modulated BSA stabilized copper nanocluster; a mechanistic perception. Anal. Chim. Acta 2018, 1031, 152–160. [Google Scholar] [CrossRef]
- Zhang, M.M.; Xu, L.Y.; Ma, Q.B.; Yu, H.; Fang, H.F.; Lin, Z.X.; Zhang, Q.L.; Chen, Z. A pH-Controlled Kit for Total and Direct Bilirubin Built on Mimetic Peroxidase CoFe2O4-DOPA-Catalyzed Fluorescence Enhancement. ACS Appl. Mater. Interfaces 2018, 10, 42155–42164. [Google Scholar] [CrossRef]
- Senthilkumar, T.; Asha, S.K. Selective and sensitive sensing of fBR in human serum using water-soluble polyfluorene as fluorescent probe. Macromolecules 2015, 48, 3449–3461. [Google Scholar] [CrossRef]
- Du, Y.; Li, X.; Lv, X.; Jia, Q. Highly sensitive and selective sensing of fBR using metal-organic frameworks-based energy transfer process. ACS Appl. Mater. Interfaces 2017, 9, 30925–30932. [Google Scholar] [CrossRef]
- Ellairaja, S.; Shenbagavalli, K.; Ponmariappan, S.; Vasantha, V.S. A green and facile approach for synthesizing imine to develop optical biosensor for wide range detection of bilirubin in human biofluids. Biosens. Bioelectron. 2017, 91, 82–88. [Google Scholar] [CrossRef]
- Chen, L.Y.; Wang, C.W.; Yuan, Z.; Chang, H.T. Fluorescent gold nanoclusters: Recent advances in sensing and imaging. Anal. Chem. 2015, 87, 216–229. [Google Scholar] [CrossRef]
- Zhuo, C.X.; Wang, L.H.; Feng, J.J.; Zhang, Y.D. Label-free fluorescent detection of trypsin activity based on DNA-stabilized silver nanocluster-peptide conjugates. Sensors 2016, 16, 1477–1486. [Google Scholar] [CrossRef]
- Li, H.; **, R.; Kong, D.; Zhao, X.; Liu, F.; Yan, X.; Lin, Y.; Lu, G. A Dual-Emission Fluorescent Nanocomplex of Gold-Cluster-Decorated Silica Particles for Live Cell Imaging of Highly Reactive Oxygen Species. J. Am. Chem. Soc. 2013, 135, 11595–11602. [Google Scholar]
- Abbas, M.A.; Kim, T.Y.; Lee, S.U.; Kang, Y.S.; Bang, J.H. Exploring interfacial events in gold-nanocluster-sensitized solar cells: Insights into the effects of the cluster size and electrolyte on solar cell performance. J. Am. Chem. Soc. 2016, 138, 390–401. [Google Scholar] [CrossRef]
- Cho, S.; Shin, H.Y.; Kim, M.I. Nanohybrids consisting of magnetic nanoparticles and gold nanoclusters as effective peroxidase mimics and their application for colorimetric detection of glucose. Biointerphases 2017, 12, 01A401. [Google Scholar] [CrossRef]
- Lin, Z.; Luo, F.; Dong, T.; Zheng, L.; Wang, Y.; Chi, Y.; Chen, G. Recyclable fluorescent gold nanocluster membrane for visual sensing of copper(ii) ion in aqueous solution. Analyst 2012, 137, 2394–2399. [Google Scholar] [CrossRef]
- Wu, R.H.; Yau, S.H.; Iii, T.G. Linear and nonlinear optical properties of monolayer-protected gold nanocluster films. ACS Nano 2016, 10, 562–572. [Google Scholar] [CrossRef]
- Pu, Z.; Yi, W.; Yin, Y. Facile fabrication of a gold nanocluster-based membrane for the detection of hydrogen peroxide. Sensors 2016, 16, 1124–1134. [Google Scholar]
- **e, J.; Zheng, Y.; Ying, J.Y. Protein-directed synthesis of highly fluorescent gold nanoclusters. J. Am. Chem. Soc. 2009, 131, 888–889. [Google Scholar] [CrossRef]
- Yan, L.; Cai, Y.; Zheng, B.; Yuan, H.; Guo, Y.; **ao, D.; Choi, M.M.F. Microwave-assisted synthesis of BSA-stabilized and HSA-protected gold nanoclusters with red emission. J. Mater. Chem. 2011, 22, 1000–1005. [Google Scholar] [CrossRef]
- Santhosh, M.; Chinnadayyala, S.R.; Kakoti, A.; Goswami, P. Selective and sensitive detection of fBR in blood serum using human serum albumin stabilized gold nanoclusters as fluorometric and colorimetric probe. Biosens. Bioelectron. 2014, 59, 370–376. [Google Scholar] [CrossRef]
- Hayashi, Y.; Kawada, Y.; Ichimura, K. Dicyanoanthracene as a fluorescence probe for studies on silica surfaces. Langmuir 1995, 11, 2077–2082. [Google Scholar] [CrossRef]
- Fang, Y.; Ning, G.; Hu, D.; Lu, J. Synthesis and solvent-sensitive fluorescence properties of a novel surface-functionalized chitosan film: Potential materials for reversible information storage. J. Photochem. Photobiol. A Chem. 2000, 135, 141–145. [Google Scholar] [CrossRef]
Sample | Determined (μM) | Spiked (μM) | Found a (μM) | RSD b (%) | Recovery c (%) |
---|---|---|---|---|---|
Sample 1 | 5.73 ± 0.34 | 5.00 | 10.96 ± 0.28 | 2.6 | 104.6 |
20.00 | 25.65 ± 1.10 | 4.3 | 99.6 | ||
Sample 2 | 7.85 ± 0.45 | 5.00 | 12.92 ± 0.31 | 2.4 | 101.4 |
20.00 | 27.88 ± 1.31 | 4.7 | 100.2 | ||
Sample 3 | 15.87 ± 1.08 | 5.00 | 20.78 ± 0.66 | 3.2 | 98.2 |
20.00 | 36.02 ± 1.30 | 3.6 | 100.6 |
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Li, Z.; **ao, W.; Huang, R.; Shi, Y.; Fang, C.; Chen, Z. A Gold Nanoclusters Film Supported on Polydopamine for Fluorescent Sensing of Free Bilirubin. Sensors 2019, 19, 1726. https://doi.org/10.3390/s19071726
Li Z, **ao W, Huang R, Shi Y, Fang C, Chen Z. A Gold Nanoclusters Film Supported on Polydopamine for Fluorescent Sensing of Free Bilirubin. Sensors. 2019; 19(7):1726. https://doi.org/10.3390/s19071726
Chicago/Turabian StyleLi, Zhou, Wenxiang **ao, Rongen Huang, Ya**g Shi, Cheng Fang, and Zhencheng Chen. 2019. "A Gold Nanoclusters Film Supported on Polydopamine for Fluorescent Sensing of Free Bilirubin" Sensors 19, no. 7: 1726. https://doi.org/10.3390/s19071726