Brassica napus Haploid and Double Haploid Production and Its Latest Applications
Abstract
:1. Introduction
2. Double Haploid
3. Haploid/Double Haploid Induction Methods
4. Brassica napus Microspore Embryogenesis
4.1. Mechanisms of Microspore Reprogramming to Embryogenesis in Brassica napus
4.1.1. Morphological Changes
4.1.2. Protein, Carbohydrate, and Lipid Changes during Microspore Embryogenesis
4.1.3. DNA Modifications
4.1.4. Gene Expression Changes
5. Chromosome Doubling
6. Brassica napus Haploid Production via Interploidy Hybridization
7. Brassica napus Haploid Induction Lines
8. Recent Findings Contributing to Brassica napus Double Haploid Plants
8.1. Plant Structure
8.2. Number of Inflorescence, Seeds, and Pod-Shattering Tendencies
8.3. Flowering Time
8.4. Seed Traits
8.5. Resistance to Diseases
8.5.1. Sclerotinia Stem Rot
8.5.2. Clubroot
8.5.3. Blackleg
9. Conclusions and Future Perspectives
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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In Vitro | Semi-In Vitro/In Vivo | In Vivo | |||
---|---|---|---|---|---|
Gynogenesis | Androgenesis | Haploid inducer lines | Interploidy hybridization | Intraspecific hybridization Interspecific hybridization | |
Flower buds Placenta Ovule Ovary | Anther Microspore | - | - | Spontaneous occurrence | |
Yield | Haploid/Double haploid | Haploid/Double haploid | Amphihaploid | Double haploid | Haploid/Double haploid |
Success rate in Brassica napus H/DH production | Low | Low/Medium | High | High | Low |
Genotype dependent | Yes | Yes | No | Yes | Yes |
Reliant on pathway conversion ability (saprophytic → sporophytic) | Yes | Yes | No | No | No |
Chromosome elimination | No | No | Yes | Yes | Yes |
Dependent on environmental conditions (i.e., medium composition, light, temperature, humidity) | Yes | Yes | No | Yes | Yes |
Pretreatment | Yes (temperature, chemical pretreatment, irradiation etc.) | Yes (temperature, chemical pretreatment, irradiation etc.) | Yes (genetic modification) | No | No |
References | [15,16] | [15,16,17,18,19] | [20,21] | [22] | [23] |
Rapeseed Population | Trait | Experimental Conditions | Used Marker Type | Number of Genes/QTLs | Author |
---|---|---|---|---|---|
Darmor-bzh × Yudal Darmor × Samourai | Blackleg resistance | Field | SNP | 16 4 | [100] |
Darmor-bzh × Yudal | Blackleg resistance | Field and greenhouse | DArT | 27 | [101] |
Darmor-bzh × Yudal | Blackleg resistance | Greenhouse | SNP | 8 | [102] |
Skipton × AgSpectrum | Blackleg resistance | Field and greenhouse | SSR, SRAP, SCAR | 8 | [103] |
Topas × AGCastle Topas × AVSapphire | Blackleg resistance | Field | SSR, DArT | 22 21 | [104] |
RP04 × Ag-Outback | Blackleg resistance | Field and greenhouse | DArT | 21 | [105] |
ECD01 (B. rapa) × DH16516 (B. napus) | Clubroot | Greenhouse | SNP, | 2 | [106] |
1CA1446.476-A1296 × Hi-Q A04-73NA × Hi-Q | Clubroot | Greenhouse | SNP | 2 | [107] |
T19 × ACDC | Clubroot | Greenhouse | SNP | 3 | [108] |
09CR500 × 09CR501 | Clubroot | Greenhouse | SNP, SSR | 2 | [109] |
Mendel × A07-26NR | Clubroot | Greenhouse | SSR, | 5 | [110] |
Zhongyou 821 × DHBao604 (H1), Zhongyou 821 × DH6576 (H2), Zhongyou 821 × Westar | Sclerotinia stem rot | Greenhouse | SNP | H1: 4–6 H2: 3–6 H3: 2–6 | [111] |
ZP1 × D12 (B. napus inberd lines) | Sclerotinia stem rot | Field | SNP | 4 | [112] |
Huashuang 5 × J7005 | Sclerotinia stem rot | Field | SSR | 13 | [113] |
Bing 409 × Zhongshuang 8 | Flowering time | Field | SNP | 5 | [114] |
IMC106RR × Wichita | Root morphology Flowering time Drought resistance | Field and greenhouse | SNP | 20 | [115] |
Polo × Topas | Flowering time Fatty acid profile Oil content | Field | SSR | 14 131 14 | [116] |
KenC-8 × N53-2 | Flowering time | Field | SNP | 55 (12 environment-stable, 43 environment-specific) | [117] |
KenC-8 × N53-2 | Multi-main stem trait | Field | SNP, SSR, STS, SRAP, IFLP | 43 | [118] |
SGDH284 × 158A (derived from Sollux and Zhoungyou9988 rapeseed cultivars) | Flowering time | Field | SNP | 56 | [119] |
Low SD line No. 935 and high SD line No. 3641 | Seed density per silique | Field | SNP | 28 | [120] |
GH06 × P174 (late flowering × early flowering | Flowering time | Field | SNP | 27 | [121] |
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Starosta, E.; Szwarc, J.; Niemann, J.; Szewczyk, K.; Weigt, D. Brassica napus Haploid and Double Haploid Production and Its Latest Applications. Curr. Issues Mol. Biol. 2023, 45, 4431-4450. https://doi.org/10.3390/cimb45050282
Starosta E, Szwarc J, Niemann J, Szewczyk K, Weigt D. Brassica napus Haploid and Double Haploid Production and Its Latest Applications. Current Issues in Molecular Biology. 2023; 45(5):4431-4450. https://doi.org/10.3390/cimb45050282
Chicago/Turabian StyleStarosta, Ewa, Justyna Szwarc, Janetta Niemann, Katarzyna Szewczyk, and Dorota Weigt. 2023. "Brassica napus Haploid and Double Haploid Production and Its Latest Applications" Current Issues in Molecular Biology 45, no. 5: 4431-4450. https://doi.org/10.3390/cimb45050282