Origin of the 6/5/6/5 Tetracyclic Cyclopiazonic Acids
Abstract
:1. Introduction
2. Results and Discussion
2.1. The 6/5/6/5 Skeleton Is Not Derived from the TE-like Pathway
2.2. AATrp Is a Shunt Metabolite
2.3. The E-Ring Cleavage Occurs after 6/5/6/5/5 Skeleton Formation
3. Materials and Methods
3.1. General Experimental Procedures
3.2. Strains and Reagents
3.3. HPLC and LC-TOFMS Analysis
3.4. Engineering of the cpaA-R* Domain
3.5. Biotransformation Assays
3.6. Isolation and Purification
3.7. Synthesis of AATrp and PAATrp
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
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Zhang, W.; Jiang, X.; Wang, M.; Zhang, Z.; Wang, N. Origin of the 6/5/6/5 Tetracyclic Cyclopiazonic Acids. Mar. Drugs 2024, 22, 74. https://doi.org/10.3390/md22020074
Zhang W, Jiang X, Wang M, Zhang Z, Wang N. Origin of the 6/5/6/5 Tetracyclic Cyclopiazonic Acids. Marine Drugs. 2024; 22(2):74. https://doi.org/10.3390/md22020074
Chicago/Turabian StyleZhang, Wenyuan, Xuejian Jiang, Minjun Wang, Zhizhen Zhang, and Nan Wang. 2024. "Origin of the 6/5/6/5 Tetracyclic Cyclopiazonic Acids" Marine Drugs 22, no. 2: 74. https://doi.org/10.3390/md22020074