Polysaccharides Extracted from Dendrobium officinale Grown in Different Environments Elicit Varying Health Benefits in Caenorhabditis elegans
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. C. elegans Strains and Maintenance
2.3. Preparation and Purification of DOP
2.4. Lifespan Assay
2.5. Hydrogen Peroxide-Induced Stress Tolerance Assay
2.6. Thermal Stress Tolerance Assay
2.7. Endoplasmic Reticulum Stress Response Assay
2.8. Analysis of α-Synuclein Protein Aggregation
2.9. Amyloid-β-Induced Paralysis Assay
2.10. Statistical Analysis
3. Results
3.1. DOP from D. officinale Cultivated in Greenhouse Extends the Lifespan of C. elegans
3.2. DOP Enhanced Resistance to Oxidative and Thermal Stress in C. elegans
3.3. DOP Upregulates ER Unfolded Protein Response in C. elegans
3.4. DOP Reduced α-Synuclein Aggregation and Delayed Amyloid-β-Induced Paralysis in C. elegans
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Source | DO/g † | DOP/mg † |
---|---|---|
TR | 0.50 ± 0.00 a | 193.63 ± 0.40 a |
GH | 0.50 ± 0.00 a | 183.57 ± 1.27 b |
RK | 0.50 ± 0.00 a | 142.73 ± 2.50 c |
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Share and Cite
Okoro, N.O.; Odiba, A.S.; Yu, Q.; He, B.; Liao, G.; **, C.; Fang, W.; Wang, B. Polysaccharides Extracted from Dendrobium officinale Grown in Different Environments Elicit Varying Health Benefits in Caenorhabditis elegans. Nutrients 2023, 15, 2641. https://doi.org/10.3390/nu15122641
Okoro NO, Odiba AS, Yu Q, He B, Liao G, ** C, Fang W, Wang B. Polysaccharides Extracted from Dendrobium officinale Grown in Different Environments Elicit Varying Health Benefits in Caenorhabditis elegans. Nutrients. 2023; 15(12):2641. https://doi.org/10.3390/nu15122641
Chicago/Turabian StyleOkoro, Nkwachukwu Oziamara, Arome Solomon Odiba, Qi Yu, Bin He, Guiyan Liao, Cheng **, Wenxia Fang, and Bin Wang. 2023. "Polysaccharides Extracted from Dendrobium officinale Grown in Different Environments Elicit Varying Health Benefits in Caenorhabditis elegans" Nutrients 15, no. 12: 2641. https://doi.org/10.3390/nu15122641