Advances and Challenges in SARS-CoV-2 Detection: A Review of Molecular and Serological Technologies
Abstract
:1. Introduction
- SARS-CoV-2 Genome and Structure
- Distribution of SARS-CoV-2 in Body Fluids and Tissues
- Collection of COVID-19 Samples
- SARS-CoV-2 Detection Methods
- A. Viral Gene Detection by Molecular Methods
2. Nucleic Acid Amplification Techniques (NAATs)
2.1. Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-qPCR)
2.2. Sequencing for Diagnostic Purposes
2.3. Digital PCR
3. Isothermal Amplification Techniques
3.1. Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP)
3.2. Nucleic Acid Sequence-Based Amplification (NASBA)
- Detection of SARS-CoV-2 antibodies
- C. SARS-CoV-2 Antigens
4. Conclusions
Funding
Conflicts of Interest
References
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El-Daly, M.M. Advances and Challenges in SARS-CoV-2 Detection: A Review of Molecular and Serological Technologies. Diagnostics 2024, 14, 519. https://doi.org/10.3390/diagnostics14050519
El-Daly MM. Advances and Challenges in SARS-CoV-2 Detection: A Review of Molecular and Serological Technologies. Diagnostics. 2024; 14(5):519. https://doi.org/10.3390/diagnostics14050519
Chicago/Turabian StyleEl-Daly, Mai M. 2024. "Advances and Challenges in SARS-CoV-2 Detection: A Review of Molecular and Serological Technologies" Diagnostics 14, no. 5: 519. https://doi.org/10.3390/diagnostics14050519