Identity of blaCTX-M Carrying Plasmids in Sequential ESBL-E. coli Isolates from Patients with Recurrent Urinary Tract Infections
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients and Isolates
2.2. Phylogenetics Analysis of ESBL E. coli
2.3. Detection of blaCTX-M Genes
2.4. Plasmid Extraction and Replicon Ty** of Plasmids
2.5. Optical DNA Map** of Plasmids
3. Results
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Patient and Isolate | Year of Isolation | Sex/Age (Year) | Time from the First Isolate (Month) | Consecutive Isolate Identical 1 | ST | PFGE—Similarity (%) 2 | Phylo Group | CTX-M Gene | Replicon Type (Inc) | Size of Plasmid (kb) |
---|---|---|---|---|---|---|---|---|---|---|
P112-1 | 2008 | M/65 | - | 131 | 50 | B2 | 27 | FIA, FIB, FII, N | 70 | |
-2 | 2 | - | 141 | B2 | 27 | FII, N | 86 | |||
P160-1 | 2011 | F/34 | - | 58 | 50 | B2 | 27 | FIA, FIB, FII, I1, Y, B/O | 104 | |
-2 | 4 | - | 43 | B1 | 27 | FIB, FII, B/O | 114 | |||
P167-1 | 2009 | F/20 | - | 2141 | 45 | F | 15 | FIA, FIB, FII, I1 | 89 | |
-2 | 1 | - | 131 | B2 | 15 | FIA, FIB, FII | 73 | |||
P179-1 | 2011 | F/2 | - | 394 | 44 | D | 15 | FII, K/B | 100 | |
-2 | 6 | - | 80 | B2 | 15 | K/B | 100 | |||
P130-1 | 2009 | F/59 | - | 131 | 61 | B2 | 15 | FIA, FIB, FII, B/O | 189 | |
-2 | 6 | - | 131 | B2 | 15 | FIA, FIB, FII, I1 | 93 | |||
-3 3 | 8 | + | 100 | B2 | 15 | FIA, FIB, FII, I1 | 93 | |||
P053-1 | 2013 | F/85 | - | 617 | 100 | A | 15 | FIA, FIB, FII | 150 | |
-2 | 5 | + | A | 15 | FIA, FIB, FII | 150 | ||||
P099-1 | 2010 | F/71 | - | 69 | 83 | D | 14 | FIB, FII, B/O | 93 | |
-2 | 6 | + | D | 14 | FIB, FII | 93 | ||||
P101-1 | 2010 | F/71 | - | 12 | 90 | B2 | 15 | FII, I1 | 94 | |
-2 | 5 | + | B2 | 15 | FII, I1 | 82 | ||||
P159-1 | 2010 | M/33 | - | 354 | 90 | F | 3 | FIA, FIB, FII | 71 | |
-2 | 6 | + | F | 3 | FIA, FIB, FII | 95 | ||||
P175-1 | 2009 | M/2 | - | 131 | 97 | B2 | 14 | FIA, FIB, FII I1 | 104 | |
-2 | 5 | + | B2 | 14 | FIA, FIB, FII I1 | 113 | ||||
P180-1 | 2012 | M/1 | - | 131 | 95 | B2 | 15 | FIA, FIB, FII | 98 | |
-2 | 5 | + | B2 | 15 | FIA, FII | 62 |
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Karami, N.; KK, S.; Yazdanshenas, S.; Lin, Y.-L.; Jaén-Luchoro, D.; Ekedahl, E.; Parameshwaran, S.; Lindblom, A.; Åhrén, C.; Westerlund, F. Identity of blaCTX-M Carrying Plasmids in Sequential ESBL-E. coli Isolates from Patients with Recurrent Urinary Tract Infections. Microorganisms 2021, 9, 1138. https://doi.org/10.3390/microorganisms9061138
Karami N, KK S, Yazdanshenas S, Lin Y-L, Jaén-Luchoro D, Ekedahl E, Parameshwaran S, Lindblom A, Åhrén C, Westerlund F. Identity of blaCTX-M Carrying Plasmids in Sequential ESBL-E. coli Isolates from Patients with Recurrent Urinary Tract Infections. Microorganisms. 2021; 9(6):1138. https://doi.org/10.3390/microorganisms9061138
Chicago/Turabian StyleKarami, Nahid, Sriram KK, Shora Yazdanshenas, Yii-Lih Lin, Daniel Jaén-Luchoro, Elina Ekedahl, Sanjana Parameshwaran, Anna Lindblom, Christina Åhrén, and Fredrik Westerlund. 2021. "Identity of blaCTX-M Carrying Plasmids in Sequential ESBL-E. coli Isolates from Patients with Recurrent Urinary Tract Infections" Microorganisms 9, no. 6: 1138. https://doi.org/10.3390/microorganisms9061138