Exploring CVD Method for Synthesizing Carbon–Carbon Composites as Materials to Contact with Nerve Tissue
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.1.1. Carbon Fibers
2.1.2. CVD Method with Direct Electrical Heating of Carbon Fibers
- CF_PyC30—rod-shaped C/C composite based on carbon fiber and PyC obtained after 30 s of synthesis.
- CF_PyC60—rod-shaped C/C composite based on carbon fiber and PyC obtained after 60 s of synthesis.
- CF_PyC120—rod-shaped C/C composite based on carbon fiber and PyC obtained after 120 s of synthesis.
- CF_PyC180—rod-shaped C/C composite based on carbon fiber and PyC obtained after 180 s of synthesis.
- CF—bundle of carbon fibers.
2.2. Methods
2.2.1. SEM and Digital Microscope
2.2.2. TEM and HRTEM
2.2.3. Selected Area Electron Diffraction (SAED)
- -
- Determining the position of the center of the diffraction pattern and the radius of the diffraction ring with indices (002);
- -
- Determining (using a self-developed script in Python) the profile of intensity changes along the perimeter of a circle with a predetermined center and radius (values read in 0.2 degree steps);
- -
- -
- Determination of their average value, equal to orientation angle OA.
2.2.4. Raman Spectroscopy
2.2.5. XPS
2.2.6. Contact Angle Measurement
2.2.7. In Vitro Study
Cell Culture and Experimental Groups
Cytotoxicity Assay
Live Cell Imaging
Cell Viability Assay
Scanning Electron Microscopy
Statistical Analysis
3. Results and Discussion
3.1. Morphology and Microstructure of Rod-Shaped C/C Composite
3.2. Structure of Rod-Shaped C/C Composite
3.3. Surface Chemistry of Rod-Shaped C/C Composite
3.4. Biocompatibility of Rod-Shaped C/C Composite
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Sample | ID | IG | I2D | ID/IG | I2D/IG | La [nm] |
---|---|---|---|---|---|---|
CF | 6148 | 17,107 | 15,937 | 0.36 | 0.93 | 37.88 |
CF_PyC120 | 7102 | 6844 | 3739 | 1.04 | 0.55 | 13.12 |
Sample | Elemental Composition (%) | (-) | Deconvolution of the C1s Spectra (%) | ||||||
---|---|---|---|---|---|---|---|---|---|
C | O | O/C | 284.5 eV C=C (sp2) | 285.3 eV C-C (sp3) | 286.1 eV C-O, C-OH | 287.0 eV C=O, O-C-O | 288.5 eV O-C=O | 291.0 eV π→π* | |
CF | 87.60 | 12.40 | 0.14 | 65.90 | 14.20 | 3.10 | 2.00 | 0.90 | 1.50 |
CF_PyC120 | 90.30 | 9.70 | 0.11 | 56.10 | 23.10 | 2.80 | 3.40 | 1.60 | 3.30 |
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Fraczek-Szczypta, A.; Kondracka, N.; Zambrzycki, M.; Gubernat, M.; Czaja, P.; Pawlyta, M.; Jelen, P.; Wielowski, R.; Jantas, D. Exploring CVD Method for Synthesizing Carbon–Carbon Composites as Materials to Contact with Nerve Tissue. J. Funct. Biomater. 2023, 14, 443. https://doi.org/10.3390/jfb14090443
Fraczek-Szczypta A, Kondracka N, Zambrzycki M, Gubernat M, Czaja P, Pawlyta M, Jelen P, Wielowski R, Jantas D. Exploring CVD Method for Synthesizing Carbon–Carbon Composites as Materials to Contact with Nerve Tissue. Journal of Functional Biomaterials. 2023; 14(9):443. https://doi.org/10.3390/jfb14090443
Chicago/Turabian StyleFraczek-Szczypta, Aneta, Natalia Kondracka, Marcel Zambrzycki, Maciej Gubernat, Pawel Czaja, Miroslawa Pawlyta, Piotr Jelen, Ryszard Wielowski, and Danuta Jantas. 2023. "Exploring CVD Method for Synthesizing Carbon–Carbon Composites as Materials to Contact with Nerve Tissue" Journal of Functional Biomaterials 14, no. 9: 443. https://doi.org/10.3390/jfb14090443