Antibacterial and Antibiofouling Activities of Carbon Polymerized Dots/Polyurethane and C60/Polyurethane Composite Films
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.4. Production of Reactive Oxygen Species
2.5. Antibacterial Activity
2.6. Antibiofilm Activity Testing
2.7. Cytotoxicity
3. Results
3.1. Surface Morphology of CPD, CPDs/PU and C60/PU Composite Films
3.2. Electrostatic Force Microscopy of CPDs, CPDs/PU and C60/PU
3.3. Chemical Composition
3.4. UV-Vis Spectra of CPD and C60 Nanoparticles
3.5. Production of Reactive Oxygen Species
3.5.1. Production of Singlet Oxygen
3.5.2. Production of OH Radicals
3.6. Antibacterial Activity of CPD/PU and C60/PU Polymer Composite Films
3.7. Antibiofouling Activity of CPDs/PU and C60/PU
3.8. Cytotoxicity
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Shineh, G.; Mobaraki, M.; Perves Bappy, M.J.; Mills, D.K. Biofilm formation, and related impacts on healthcare, food Processing and packaging, industrial manufacturing, marine industries, and sanitation—A review. Appl. Microbiol. 2023, 3, 629–665. [Google Scholar] [CrossRef]
- Percival, L.; Suleman, L.; Vuotto, C.; Donelli, G. Healthcare-associated infections, medical devices and biofilms: Risk, tolerance and control. J. Med. Microbiol 2015, 64, 323–334. [Google Scholar] [CrossRef]
- Wißmann, J.E.; Kirchhoff, L.; Brüggemann, Y.; Todt, D.; Steinmann, J.; Steinmann, E. Persistence of pathogens on inanimate surfaces: A narrative review. Microorganisms 2021, 9, 343. [Google Scholar] [CrossRef]
- Assefa, M.; Amare, A. Biofilm-associated multi-drug resistance in hospital-acquired infections: A review. Infect. Drug Resist. 2022, 15, 5061–5068. [Google Scholar] [CrossRef]
- Cruz-Lopez, F.; Martinez-Melendez, A.; Garza-Gonzalez, E. How does hospital microbiota contribute to healthcare-associated infections? Microorganisms 2023, 11, 192. [Google Scholar] [CrossRef] [PubMed]
- Cruz-Lopez, F.; Villarreal-Treviño, L.; Morfin-Otero, R.; Martínez-Meléndez, A.; Camacho-Ortiz, A.; Rodríguez-Noriega, E.; Garza-González, E. Microbial diversity and colonization patterns of two step-down care units from a tertiary care hospital. J. Res. Med. Sci. 2021, 26, 126. [Google Scholar] [CrossRef] [PubMed]
- Jabłońska-Trypuć, A.; Makuła, M.; Włodarczyk-Makuła, M.; Wołejko, E.; Wydro, U.; Serra-Majem, L.; Wiater, J. Inanimate surfaces as a source of hospital infections caused by fungi, bacteria and viruses with particular emphasis on SARS-CoV-2. Int. J. Environ. Res. Public Health 2022, 19, 8121. [Google Scholar] [CrossRef]
- Kurashige, E.J.O.; Oie, S.; Furukawa, H. Contamination of environmental surfaces by methicillin-resistant Staphylococcus aureus (MRSA) in rooms of inpatients with MRSA-positive body sites. Braz. J. Microbiol. 2016, 47, 703–705. [Google Scholar] [CrossRef]
- Fazeli, H.; Akbari, R.; Moghim, S.; Narimani, T.; Arabestani, M.R.; Ghoddousi, A.R. Pseudomonas aeruginosa infections in patients, hospital means, and personnel’s specimens. J. Res. Med. Sci. 2012, 17, 332–337. [Google Scholar]
- Huang, L.; Tang, J.; Tian, G.; Tao, H.; Li, Z. Risk factors, outcomes, and predictions of extensively drug-resistant Acinetobacter baumannii nosocomial infections in patients with nervous system diseases. Infect. Drug Resist. 2023, 16, 7327–7337. [Google Scholar] [CrossRef]
- Kramer, A.; Assadian, O. Survival of Microorganisms on Inanimate Surfaces. In Use of Biocidal Surfaces for Reduction of Healthcare Acquired Infections; Borkow, G., Ed.; Springer International Publishing: Berlin/Heidelberg, Germany, 2014; pp. 7–26. [Google Scholar] [CrossRef]
- Zheng, S.; Bawazir, M.; Dhall, A.; Kim, H.E.; He, L.; Heo, J.; Hwang, G. Implication of surface properties, bacterial motility, and hydrodynamic conditions on bacterial surface sensing and their initial adhesion. Front. Bioeng. Biotechnol. 2021, 9, 643722. [Google Scholar] [CrossRef] [PubMed]
- Donlan, R.M. Biofilms: Microbial life on surfaces. Emerg. Infect. Dis. 2002, 8, 881–890. [Google Scholar] [CrossRef]
- Yin, W.; Xu, S.; Wang, Y.; Zhang, Y.; Chou, S.H.; Galperin, M.Y.; He, J. Ways to control harmful biofilms: Prevention, inhibition, and eradication. Crit. Rev. Microbiol. 2021, 47, 57–78. [Google Scholar] [CrossRef]
- Dai, T.; Huang, Y.; Hamblin, M.R. Photodynamic therapy for localized infections−State of the art. Photodiagn. Photodyn. Ther. 2009, 6, 170–188. [Google Scholar] [CrossRef]
- Maisch, T.; Baier, J.; Franz, B.; Maier, M.; Landthaler, M.; Szeimies, R.; Bäumler, W. The role of singlet oxygen and oxygen concentration in photodynamic inactivation of bacteria. Proc. Natl. Acad. Sci. USA 2007, 104, 7223–7228. [Google Scholar] [CrossRef]
- Kováčová, M.; Marković, Z.M.; Humpolíček, P.; Mičušík, M.; Švajdlenková, H.; Kleinová, A.; Danko, M.; Kubát, P.; Vajďák, J.; Capáková, Z.; et al. Carbon quantum dots modified polyurethane nanocomposite as effective photocatalytic and antibacterial agents. ACS Biomater. Sci. Eng. 2018, 4, 3983–3993. [Google Scholar] [CrossRef]
- Marković, Z.M.; Kováčová, M.; Jeremić, S.R.; Nagy, Š.; Milivojević, D.D.; Kubat, P.; Kleinová, A.; Budimir, M.D.; Mojsin, M.M.; Stevanović, M.J.; et al. Highly efficient antibacterial polymer composites based on hydrophobic riboflavin carbon polymerized dots. Nanomaterials 2022, 12, 4070. [Google Scholar] [CrossRef]
- Savelyev, Y.; Gonchar, A.; Movchan, B.; Gornostay, A.; Vozianov, S.; Rudenko, A.; Rozhnova, R.; Travinskaya, T. Antibacterial polyurethane materials with silver and copper nanoparticles. Mater. Proc. 2017, 4, 87–94. [Google Scholar] [CrossRef]
- Miranda, C.; Castano, J.; Valdebenito-Rolack, E.; Sanhueza, F.; Toro, R.; Bello-Toledo, H.; Uarac, P.; Saez, L. Copper-polyurethane composite materials: Particle size effect on the physical-chemical and antibacterial properties. Polymers 2020, 12, 1934. [Google Scholar] [CrossRef] [PubMed]
- Saleemi, M.A.; Lim, V. Overview of antimicrobial polyurethane-based nanocomposite materials and associated signaling pathways. Eur. Polym. J. 2022, 167, 111087. [Google Scholar] [CrossRef]
- Farrokhi, Z.; Ayati, A.; Kanvisi, M.; Sillanpää, M. Recent advance in antibacterial activity of nanoparticles contained polyurethane. J. Appl. Polym. Sci. 2018, 136, 46997. [Google Scholar] [CrossRef]
- Ghirardello, M.; Ramos-Soriano, J.; Galan, M.C. Carbon dots as an emergent class of antimicrobial agents. Nanomaterials 2021, 11, 1877. [Google Scholar] [CrossRef] [PubMed]
- Zhang, X.; Cong, H.; Yu, B.; Chen, Q. Recent advances of water-soluble fullerene derivatives in biomedical applications. Mini-Rev. Org. Chem. 2019, 16, 92–99. [Google Scholar] [CrossRef]
- Díez-Pascual, A.M. State of the art in the antibacterial and antiviral applications of carbon-based polymeric nanocomposites. Int. J. Mol. Sci. 2021, 22, 10511. [Google Scholar] [CrossRef] [PubMed]
- Nečas, D.; Klapetek, P. Gwyddion: Open-source software for SPM data analysis. Cent. Eur. J. Phys. 2012, 10, 181–218. [Google Scholar] [CrossRef]
- AM-FM Viscoelastic Map** Mode. Available online: https://afm.oxinst.com/assets/uploads/products/asylum/documents/AM-FM-Viscoelastic-Map**-Mode-Application-Note.pdf (accessed on 2 February 2024).
- Moulder, J.F.; Stickle, W.F.; Sobol, P.E.; Bomben, K.D. Handbook of X-Ray Photoelectron Spectroscopy; Physical Electronics Inc.: Eden Prairie, MN, USA, 1995. [Google Scholar]
- Lin, H.; Shen, Y.; Chen, D.; Lin, L.; Wilson, B.C.; Li, B.; ** of individual carbon nanotubes in polymer/nanotube composites using electrostatic force microscopy. Appl. Phys. Lett. 2007, 90, 183108. [Google Scholar] [CrossRef]
- Govindaraj, P.; Sokolova, A.; Salim, N.; Juodkazis, S.; Fuss, F.K.; Fox, B.; Hameed, N. Distribution states of graphene in polymer nanocomposites: A review. Compos. Part B Eng. 2021, 226, 109353. [Google Scholar] [CrossRef]
- Onoe, J.; Nakao, A.; Takeuchi, K. XPS study of a photopolymerized C60 film. Phys. Rev. B 1997, 55, 10051–10056. [Google Scholar] [CrossRef]
- Infrared Spectroscopy Absorption Table. Available online: https://chem.libretexts.org/Ancillary_Materials/Reference/Reference_Tables/Spectroscopic_Reference_Tables/Infrared_Spectroscopy_Absorption_Table (accessed on 3 February 2024).
- Gao, Y.; Jiao, Y.; Lu, W. Carbon dots with red emission as a fluorescent and colorimetric dual-readout probe for the detection of chromium (VI) and cysteine and its logic gate operation. J. Mater. Chem. B 2018, 6, 6099–6107. [Google Scholar] [CrossRef] [PubMed]
- Nguyen, K.G.; Baragau, I.A.; Gromicova, R.; Nicolaev, A.; Thompson, S.A.J.; Rennie, A.; Power, N.P.; Sajjad, M.T. Investigating the effect of N-do** on carbon quantum dots structure, optical properties and metal ion screening. Sci. Rep. 2022, 12, 13806. [Google Scholar] [CrossRef]
- Sarkar, S.; Sudolská, M.; Dubecký, M.; Reckmeier, C.J.; Rogach, A.L.; Zbořil, R.; Otyepka, M. Graphitic nitrogen do** in carbon dots causes red-shifted absorption. J. Phys. Chem. C 2016, 120, 1303–1308. [Google Scholar] [CrossRef]
- Jovanović, S.; Marković, Z.; Budimir, M.; Prekodravac, J.; Zmejkoski, D.; Kepić, D.; Bonasera, A.; Todorović Marković, B. Lights and Dots toward Therapy—Carbon-based quantum dots as new agents for photodynamic therapy. Pharmaceutics 2023, 15, 1170. [Google Scholar] [CrossRef]
- Ge, J.; Lan, M.; Zhou, B.; Liu, W.; Guo, L.; Wang, H.; Jia, Q.; Niu, G.; Huang, X.; Zhou, H.; et al. A Graphene quantum dot photodynamic therapy agent with high singlet oxygen generation. Nat. Commun. 2014, 5, 4596. [Google Scholar] [CrossRef]
- Chong, Y.; Ge, C.; Fang, G.; Tian, X.; Ma, X.; Wen, T.; Wamer, W.G.; Chen, C.; Chai, Z.; Yin, J.J. Crossover between anti- and pro-oxidant activities of graphene quantum dots in the absence or presence of light. ACS Nano 2016, 10, 8690–8699. [Google Scholar] [CrossRef]
- Hong, Y.; Zeng, J.; Wang, X.; Drlica, K.; Zhao, X. Post-stress bacterial cell death mediated by reactive oxygen species. Proc. Natl. Acad. Sci. USA 2019, 116, 10064–10071. [Google Scholar] [CrossRef]
- Colombo, I.; Sangiovanni, E.; Maggio, R.; Mattozzi, C.; Zava, S.; Corbett, Y.; Fumagalli, M.; Carlino, C.; Antonia Corsetto, P.; Scaccabarozzi, D.; et al. HaCaT cells as a reliable in vitro differentiation model to dissect the inflammatory/repair response of human keratinocytes. Mediators Inflamm. 2017, 2017, 7435621. [Google Scholar] [CrossRef]
- Hanel, K.H.; Cornelissen, C.; Luscher, B.; Baron, J.M. Cytokines and the skin barrier. Int. J. Mol. Sci. 2013, 14, 6720–6745. [Google Scholar] [CrossRef] [PubMed]
- Schurer, N.; Kohne, A.; Schliep, V.; Barlag, K.; Goerz, G. Lipid composition and synthesis of HaCaT cells, an immortalized human keratinocyte line, in comparison with normal human adult keratinocytes. Exp. Dermatol. 1993, 2, 179–185. [Google Scholar] [CrossRef] [PubMed]
- Jost, V. Packaging related properties of commercially available biopolymers—An overview of the status quo. eXPRESS Polym. Lett. 2018, 12, 429–435. [Google Scholar] [CrossRef]
- Philpott, D.J.; Edgeworth, J.D.; Sansonetti, P.J. The pathogenesis of Shigella flexneri infection: Lessons from in vitro and in vivo studies. Philos. Trans. R. Soc. Lond. B Biol. Sci. 2000, 355, 575–586. [Google Scholar] [CrossRef] [PubMed]
- Stanković, N.K.; Bodik, M.; Šiffalovič, P.; Kotlar, M.; Mičušik, M.; Špitalsky, Z.; Danko, M.; Milivojević, D.D.; Kleinova, A.; Kubat, P.; et al. Antibacterial and antibiofouling properties of light triggered fluorescent hydrophobic carbon quantum dots Langmuir−Blodgett thin films. ACS Sustain. Chem. Eng. 2018, 6, 4154–4163. [Google Scholar] [CrossRef]
- Xu, X.; Cao, R.; Li, K.; Wan, Q.; Wu, G.; Lin, Y.; Huang, T.; Wen, G. The protective role and mechanism of melanin for Aspergillus niger and Aspergillus flavus against chlorine-based disinfectants. Wat. Res. 2022, 223, 119039. [Google Scholar] [CrossRef]
CPD | C60 | |
---|---|---|
Element | At% | At% |
C 1 s | 83.1 | 90.0 |
O 1 s | 7.3 | 10.0 |
N 1 s | 9.6 | 0.0 |
Characteristic Bond | Binding Energy (eV) | Relative Concentration (%) | Characteristic Bond | Binding Energy (eV) | Relative Concentration (%) |
---|---|---|---|---|---|
CPDs | C60 | ||||
C 1 s peak C-C/C-H | 284.5 | 80 | C 1 s peak C-C/C-H | 284.5 | 79 |
C 1 s peak C=O | 286.5 | 20 | C 1 s peak C-O/C-OH | 285.9 | 20 |
O 1 s peak C=O | 531.4 | 100 | C1 s peak C-O/C-OH | 288.7 | 1 |
N 1 s peak pyridinic | 398.7 | 45 | O 1 s peak O-H | 532.2 | 87 |
N 1 s peak pyrrolic | 399.9 | 49 | O 1 s peak C-O/H2O | 533.3 | 13 |
N 1 s peak C=N-C | 396.4 | 6 | - | - | - |
Bacteria Strains | Irradiated, Incubated N (cell/cm2) | Nonirradiated, Incubated N (cell/cm2) | R | Irradiated, Incubated N (cell/cm2) | Nonirradiated, Incubated N (cell/cm2) | R |
---|---|---|---|---|---|---|
CPDs/PU | C60/PU | |||||
K. pneumoniae | 0 | 0 | 5.45 | 0 | 0 | 5.45 |
P. mirabilis | 0 | 20 × 106 | 5.52 | 0 | 0 | 5.52 |
S. enterica | 0 | 5 × 107 | 4.81 | 0 | 2 × 107 | 4.81 |
E. faecalis | 0 | 10 × 105 | 5.53 | 0 | 6 × 105 | 5.53 |
E. epidermis | 0 | 0 | 4.55 | 0 | 2 × 106 | 0.11 |
S. flexneri | 64 × 102 | 82 × 104 | 2.10 | 7.6 × 103 | 9.8 × 105 | 2.12 |
P. aeruginosa | 0 | 70 × 105 | 4.95 | 0 | 1 × 105 | 4.95 |
A. niger | 8.7 × 102 | 1 × 105 | 2.06 | 0 | 0 | 5.47 |
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Marković, Z.M.; Budimir Filimonović, M.D.; Milivojević, D.D.; Kovač, J.; Todorović Marković, B.M. Antibacterial and Antibiofouling Activities of Carbon Polymerized Dots/Polyurethane and C60/Polyurethane Composite Films. J. Funct. Biomater. 2024, 15, 73. https://doi.org/10.3390/jfb15030073
Marković ZM, Budimir Filimonović MD, Milivojević DD, Kovač J, Todorović Marković BM. Antibacterial and Antibiofouling Activities of Carbon Polymerized Dots/Polyurethane and C60/Polyurethane Composite Films. Journal of Functional Biomaterials. 2024; 15(3):73. https://doi.org/10.3390/jfb15030073
Chicago/Turabian StyleMarković, Zoran M., Milica D. Budimir Filimonović, Dušan D. Milivojević, Janez Kovač, and Biljana M. Todorović Marković. 2024. "Antibacterial and Antibiofouling Activities of Carbon Polymerized Dots/Polyurethane and C60/Polyurethane Composite Films" Journal of Functional Biomaterials 15, no. 3: 73. https://doi.org/10.3390/jfb15030073