VEGF Detection via Simplified FLISA Using a 3D Microfluidic Disk Platform
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fabrication of 3D-Printed Block and Post Treatment
2.2. Assembly of the 3D Microfluidic Disk
2.3. Preparation of VEGF Reagents
2.4. Analysis and Detection of Fluorescence
3. Results and Discussion
3.1. Validation of the Simplified Microbead FLISA Protocol
3.2. One-Step Simplified Microbead FLISA Using a 3D Microfluidic Disk
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
BSA | Bovine Serum Albumin |
cAb | Capture Antibody |
dAb | Detection Antibody |
ELISA | Enzyme-Linked Immunosorbent Assay |
FLISA | Fluorescence-Linked Immunosorbent Assay |
PMMA | Poly(methyl methacrylate) |
PBS | Phosphate Buffer Saline |
PBST | Phosphate Buffer Saline with Tween Detergent |
PSA | Pressure-Sensitive Adhesive |
SLA | Stereo-Lithography Apparatus |
VEGF | Vascular Endothelial Growth Factor |
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Kang, D.H.; Kim, N.K.; Park, S.-W.; Kang, H.W. VEGF Detection via Simplified FLISA Using a 3D Microfluidic Disk Platform. Biosensors 2021, 11, 270. https://doi.org/10.3390/bios11080270
Kang DH, Kim NK, Park S-W, Kang HW. VEGF Detection via Simplified FLISA Using a 3D Microfluidic Disk Platform. Biosensors. 2021; 11(8):270. https://doi.org/10.3390/bios11080270
Chicago/Turabian StyleKang, Dong Hee, Na Kyong Kim, Sang-Woo Park, and Hyun Wook Kang. 2021. "VEGF Detection via Simplified FLISA Using a 3D Microfluidic Disk Platform" Biosensors 11, no. 8: 270. https://doi.org/10.3390/bios11080270