Harderian Gland Development and Degeneration in the Fgf10-Deficient Heterozygous Mouse
Abstract
:1. Introduction
2. Materials and Methods
2.1. Genoty** of Fgf10-Mutant Mice
2.2. Fixation, Sectioning, and Histology
2.3. Immunohistochemistry
2.4. TUNEL Staining
2.5. In Situ Hybridization (ISH)
2.6. Microscopy and Camera/Imaging System
2.7. Statistics
3. Results
3.1. More Than Half the Cases of Fgf10+/− HGs Exhibiting, often Unilaterally, the Loss of Glandular Cells, Fibrosis and Melanocytic Hyperplasia as Early as Postnatal Day Six
3.2. More Than Half the Cases of Fgf10+/− HGs Exhibit the Unilateral Absence of HG Development Just before or after Birth
3.3. A Re-Examination of the Expression Pattern of Fgf10 in Association with Fgfr2b and Fgf7 during Mouse HG Development
3.4. Fgf10 Expression in the Harderian Mesenchyme Lacking Develo** Glandular Cells Just before Birth
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ikeda, S.; Sato, K.; Fujita, H.; Ono-Minagi, H.; Miyaishi, S.; Nohno, T.; Ohuchi, H. Harderian Gland Development and Degeneration in the Fgf10-Deficient Heterozygous Mouse. J. Dev. Biol. 2024, 12, 16. https://doi.org/10.3390/jdb12020016
Ikeda S, Sato K, Fujita H, Ono-Minagi H, Miyaishi S, Nohno T, Ohuchi H. Harderian Gland Development and Degeneration in the Fgf10-Deficient Heterozygous Mouse. Journal of Developmental Biology. 2024; 12(2):16. https://doi.org/10.3390/jdb12020016
Chicago/Turabian StyleIkeda, Shiori, Keita Sato, Hirofumi Fujita, Hitomi Ono-Minagi, Satoru Miyaishi, Tsutomu Nohno, and Hideyo Ohuchi. 2024. "Harderian Gland Development and Degeneration in the Fgf10-Deficient Heterozygous Mouse" Journal of Developmental Biology 12, no. 2: 16. https://doi.org/10.3390/jdb12020016