Subcellular Proteomics to Elucidate Soybean Response to Abiotic Stress
Abstract
:1. Introduction
2. Morphophysiological Changes of Soybean under Abiotic Stress
2.1. Morphophysiological Changes of Soybean under Flooding Stress
2.2. Morphophysiological Changes of Soybean under Drought Stress
2.3. Morphophysiological Changes of Soybean under Salt Stress
2.4. Morphophysiological Changes of Soybean under Low-Temperature Stress
2.5. Morphophysiological Changes of Soybean under High-Temperature Stress
2.6. Morphophysiological Changes of Soybean under Other Stresses
3. Subcellular Response in Soybean under Abiotic Stress
3.1. The Response of Endoplasmic Reticulum in Soybean under Abiotic Stress
3.2. The Response of Chloroplast in Soybean under Abiotic Stress
3.3. The Response of Mitochondria in Soybean under Abiotic Stress
3.4. The Response of Nucleus in Soybean under Abiotic Stress
3.5. The Response of Plasma Membrane in Soybean under Abiotic Stress
3.6. The Response of Cell Wall in Soybean under Abiotic Stress
3.7. The Response of Other Organelles in Soybean under Abiotic Stress
4. Proteins Regulated Organellar Stress among Subcellular Compartments in Soybean under Abiotic Stress
4.1. Reactive Oxygen Species
4.2. Molecular Chaperons
4.3. Other Proteins
5. Phytohormone Signals in Soybean under Abiotic Stress
6. Concluding Remarks
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Subcellular | Organ | Plant | Stress | Technique | Findings | Ref. |
---|---|---|---|---|---|---|
ER | root tip | 2-day-old | flood/2 days | 1DE LC–MS/ MS, gel-free LC–MS/MS | Under flood conditions, 117 and 212 proteins increased and decreased, and 111 proteins were enriched in post-translational modification, protein synthesis, folding, degradation, and activation. | [114] |
ER | root tip | 2-day-old | flood/2 days, drought/2 days | gel-free/label-free LC–MS/MS | A number of 368 and 103 proteins were identified under flood conditions and drought, and proteins enriched in protein glycosylation and signal responded to both stresses. | [27] |
Chloroplast | leaf | 10-day-old | O3 (120 ppb)/3 days | 2DE MALDI–TOF/MS | A total of 32 proteins were responsive to ozone stress, and proteins associated with photosynthesis mostly decreased. | [23] |
Mitochondrion | root and hypocotyl | 2-day-old | flood/2 days | 2DE, BN-PAGE LC–MS/MS | In mitochondria, 34 matrix proteins and 16 membrane proteins changed by flood conditions, and proteins related to the tricarboxylic acid cycle increased, while inner membrane carriers and proteins related to complexes III, IV, and V of electron transport chains decreased. | [115] |
Mitochondrion | root tip | 2-day-old | flood with A12O3 (5, 30–60, 135 nm at 50 ppm)/2 days | gel-free/ label-free LC–MS/MS | Under flood conditions, six, five, and six proteins were specifically changed in plants exposed to 5, 30–60, and 135 nm A12O3, indicating various sizes of nanoparticles affected membrane permeability and the tricarboxylic acid cycle activity through mediating mitochondrial proteins. | [25] |
Nucleus | root tip | 2-day-old | flood/3, 6, 24 h | gel-free/label-free LC–MS/MS | A total of 237 proteins were altered by flood conditions in a time–course manner, while 365 proteins were changed by the initial 3 h flood and mapped to pre-mRNA processing and pre-ribosome biogenesis. | [116] |
Nucleus | root tip | 2-day-old | flood/3 h | gel-free/label-free LC–MS/MS | Under flood conditions, 14 nuclear phosphoproteins were changed, and phosphorylation of zinc finger/BTB domain-containing protein 47 was responsive to activated ABA signal for stress tolerance. | [117] |
Plasma membrane | leaf and root | 7-day-old | NaCl (50, 100 mM)/7 days | gel-free/label-free LC–MS/MS | Under the presence of salt, 140 and 57 proteins were specific to the root and leaf, and they were mainly involved in transport inside the cell. | [118] |
Plasma membrane | root tip | 14-day-old | AlCl3 (50 μM in 0.5 mM CaCl2)/3 days | TMT LC–MS/MS | A total of 90 proteins were differentially accumulated and enriched in membrane trafficking/transporters, cell wall modification, defense response, and signal transduction. | [119] |
Cell wall | root and hypocotyl | 2-day-old | flood/2 days | 2DE MALDI–TOF/MS | Under flood conditions, 16 proteins responded with decreased stem 28/31 kDa glycoprotein precursors, germin-like protein precursors, and increased methionine synthases. | [120] |
Peroxisome | cotyledon | seed | dark/7 day | 2DE MALDI–TOF/MS | Identified peroxisomal proteins harboring peroxisomal targeting signal sequences were associated with the beta-oxidation cycle, glyoxylate cycle, and stress response. | [121] |
Peroxisome | cotyledon | seed | dark/7 day | BN-PAGE MALDI–TOF/MS | GmPNC1 was identified, and PNC1 contributed to the transport of adenine nucleotides consumed during post-germinative growth. | [122] |
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Wang, X.; Komatsu, S. Subcellular Proteomics to Elucidate Soybean Response to Abiotic Stress. Plants 2023, 12, 2865. https://doi.org/10.3390/plants12152865
Wang X, Komatsu S. Subcellular Proteomics to Elucidate Soybean Response to Abiotic Stress. Plants. 2023; 12(15):2865. https://doi.org/10.3390/plants12152865
Chicago/Turabian StyleWang, **n, and Setsuko Komatsu. 2023. "Subcellular Proteomics to Elucidate Soybean Response to Abiotic Stress" Plants 12, no. 15: 2865. https://doi.org/10.3390/plants12152865