Analysis of the Genetic Diversity in Tea Plant Germplasm in Fujian Province Based on Restriction Site-Associated DNA Sequencing
Abstract
:1. Introduction
2. Result
2.1. Overview of Sequencing Quality of 70 Tea Plant Cultivars
2.2. SNP Variation and Genetic Relationships Analysis of 70 Tea Plant Cultivars
2.3. Selective Pressure Analysis
2.4. Overall Profile of Volatiles in 70 Tea Plant Cultivars
2.5. Analysis of Characteristic Volatile in 70 Tea Plant Cultivars
2.6. MGWAS Analysis and Candidate Gene Screening
2.7. Screening and Functional Analysis of Volatile-Associated Genes Subject to Selection Pressure
3. Discussion
3.1. Extensive Exchanges between the Tea Plant Cultivars in Fujian and Other Regions
3.2. Genes Related to Quality and Photosynthesis of Tea Plants May Be Selected during Evolutionary Processes
3.3. Selection-Pressured Volatile-Associated Genes May Be Markers for Screening Highly Aromatic Tea Plant Cultivars
4. Materials and Methods
4.1. Sample Collection
4.2. DNA Extraction, Library Construction, Sequencing, and Data Processing
4.3. Population Structure, Principal Component, and Phylogenetic Tree Construction Analysis
4.4. Select Pressure Analysis
4.5. Analysis of Volatiles
4.6. Genome-Wide Association Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Trait | Mean | Standard Deviation | Standard Error | Median | Min | Max | Range | Skew | Kyrtosis |
---|---|---|---|---|---|---|---|---|---|
α-farnesene | 965.16 | 1710.24 | 204.41 | 300.42 | 5.5 | 10,144.3 | 10,138.79 | 3.45 | 13.75 |
3-Carene | 2804.67 | 4639.92 | 554.58 | 1260.3 | 29.59 | 33,451.74 | 33,422.15 | 4.38 | 24.73 |
Cis-2-pentenol-1-ol | 315.86 | 363.03 | 43.39 | 132.32 | 12.09 | 1681.63 | 1669.54 | 1.69 | 2.72 |
(Z)-linalool oxide | 258.42 | 362.56 | 43.33 | 129.3 | 8.39 | 2141.99 | 2133.6 | 2.82 | 9.68 |
(E,E)-2,4-hexadienal | 168.19 | 183.01 | 22.03 | 89.2 | 6.43 | 809.91 | 803.48 | 1.56 | 1.91 |
(E)-4-hexen-ol | 879.54 | 1552.29 | 185.53 | 353.97 | 7.99 | 8209.08 | 8201.09 | 3.04 | 9.39 |
(E)-linalool oxide | 615.06 | 920.17 | 109.98 | 272.16 | 19.98 | 6074.06 | 6054.08 | 3.06 | 15.51 |
Hexanal | 184.14 | 244.71 | 29.25 | 73.66 | 3.89 | 1314.8 | 1337.91 | 2.23 | 5.88 |
Cis-3-hexen-1-ol | 768.71 | 1054.45 | 126.03 | 350.32 | 1.61 | 6203.63 | 6202.02 | 2.57 | 8.55 |
Methyl salicylate | 149.29 | 247.97 | 29.64 | 52.39 | 6.24 | 1585.38 | 1579.14 | 3.63 | 15.81 |
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Jiang, L.; **e, S.; Zhou, C.; Tian, C.; Zhu, C.; You, X.; Chen, C.; Lai, Z.; Guo, Y. Analysis of the Genetic Diversity in Tea Plant Germplasm in Fujian Province Based on Restriction Site-Associated DNA Sequencing. Plants 2024, 13, 100. https://doi.org/10.3390/plants13010100
Jiang L, **e S, Zhou C, Tian C, Zhu C, You X, Chen C, Lai Z, Guo Y. Analysis of the Genetic Diversity in Tea Plant Germplasm in Fujian Province Based on Restriction Site-Associated DNA Sequencing. Plants. 2024; 13(1):100. https://doi.org/10.3390/plants13010100
Chicago/Turabian StyleJiang, Lele, Siyi **e, Chengzhe Zhou, Caiyun Tian, Chen Zhu, **aomei You, Changsong Chen, Zhongxiong Lai, and Yuqiong Guo. 2024. "Analysis of the Genetic Diversity in Tea Plant Germplasm in Fujian Province Based on Restriction Site-Associated DNA Sequencing" Plants 13, no. 1: 100. https://doi.org/10.3390/plants13010100