Heat Stress-Tolerant Quantitative Trait Loci Identified Using Backcrossed Recombinant Inbred Lines Derived from Intra-Specifically Diverse Aegilops tauschii Accessions
Abstract
:1. Introduction
2. Results
2.1. Climate Conditions during the Growing Seasons
2.2. Impact of Heat Stress on the BIL Populations
2.3. Relationship among Traits
2.4. Linkage Maps for the BILs
2.5. Identified QTLs in All Environments
2.6. QTLs Associated with Heat Stress Response in Both BILs
3. Discussion
3.1. The High-Resolution Linkage Maps
3.2. QTLs Identified in All Environments
3.2.1. Identification of Stable Major QTLs for Yield- and Heat Stress Tolerance-Related Traits
3.2.2. Common and Specific Regions of Detected QTLs in BIL1 and BIL2
4. Materials and Methods
4.1. Plant Materials
4.2. Experimental Sites and Design
4.3. Phenoty** of BIL Populations
4.3.1. Trait Evaluation
4.3.2. Statistical Analysis of Phenotypic Data
4.4. Genoty** of the BILs, Map Construction, and QTL Analysis
4.4.1. DNA Extraction
4.4.2. Maps Construction
4.4.3. QTL Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chr 1 | Trait | Pop 2 | Pos 3 (cM) | Left Marker | Right Marker | LOD 4 | PVE (%) 5 | Add 6 | Co-Localized with |
---|---|---|---|---|---|---|---|---|---|
1A | STI1-GY | BIL2(BLUP) | 144 | AMP0028912 | AMP0002760 | 3.35 | 8.98 | −0.29 | |
1A | STI1-TKW | BIL1 | 119 | AMP0036610 | AMP0034796 | 4.37 | 14.37 | 0.12 | Guan et al. [23] |
1A | STI1-TKW | BIL1 | 173 | AMP0035547 | AMP0004300 | 2.98 | 9.89 | −0.10 | Guan et al. [23] |
1A | STI1-TKW | BIL1 | 118 | AMP0036610 | AMP0034796 | 3.74 | 11.37 | 0.11 | Guan et al. [23] |
1A | STI1-TKW | BIL1 | 173 | AMP0035547 | AMP0004300 | 4.27 | 14.02 | −0.11 | Guan et al. [23] |
1A | STI2-TKW | BIL1 | 173 | AMP0035547 | AMP0004300 | 4.82 | 14.54 | −0.10 | Guan et al. [23] |
1A | STI1-BIO | BIL1 | 148 | AMP0034796 | AMP0020845 | 2.64 | 5.14 | −0.12 | |
1B | STI1-GY | BIL1 | 144 | AMP0017578 | AMP0023142 | 2.60 | 7.49 | 0.07 | |
1D | STI1-GY | BIL1 | 117 | AMP0027815 | AMP0029085 | 5.23 | 15.44 | −0.10 | |
1D | STI2-TKW | BIL1 | 236 | AMP0005955 | AMP0027742 | 3.52 | 10.14 | −0.09 | |
2B | STI1-TKW | BIL1 | 63 | AMP0009891 | AMP0006464 | 3.21 | 10.41 | 0.10 | |
2B | STI1-TKW | BIL1 | 62 | AMP0009891 | AMP0006464 | 2.95 | 8.66 | 0.08 | Paliwal et al. [24] |
2B | STI1-BIO | BIL2 | 150 | AMP0012513 | AMP0026808 | 2.80 | 3.46 | −0.17 | |
2D | STI1-TKW | BIL2 | 21 | AMP0020907 | AMP0024533 | 4.11 | 3.15 | 0.19 | Guan et al. [23] |
2D | STI1-GY | BIL2 (BLUP) | 152 | AMP0017649 | AMP0022052 | 2.51 | 6.72 | −0.07 | |
3A | STI1-GY | BIL2 | 49 | AMP0010424 | AMP0003988 | 5.33 | 14.20 | 0.10 | |
3A | STI1-TKW | BIL2 | 129 | AMP0014988 | AMP0016989 | 18.63 | 15.53 | −0.19 | |
3A | STI1-TKW | BIL2 | 137 | AMP0029972 | AMP0030211 | 10.85 | 8.05 | 0.14 | |
3A | STI1-TKW | BIL2 | 178 | AMP0007900 | AMP0004728 | 4.15 | 2.82 | −0.07 | |
3A | STI2-TKW | BIL2 | 39 | AMP0030786 | AMP0010424 | 2.83 | 6.29 | 0.33 | |
3A | STI1-BIO | BIL2 | 49 | AMP0010424 | AMP0003988 | 3.03 | 0.57 | 0.07 | |
3D | STI2-BIO | BIL2 | 300 | AMP0001446 | AMP0012860 | 4.58 | 2.97 | −0.31 | |
4B | STI2-GY | BIL2 | 140 | AMP0018665 | AMP0020290 | 3.27 | 8.54 | 0.07 | |
4B | STI1-BIO | BIL2 | 176 | AMP0025189 | AMP0026555 | 3.92 | 1.12 | −0.28 | |
4B | STI2-BIO | BIL2 | 178 | AMP0026555 | AMP0003848 | 5.10 | 2.89 | −0.36 | |
4D | STI1-TKW | BIL2 | 93 | AMP0031292 | AMP0028457 | 2.56 | 1.70 | −0.08 | |
4D | STI2-BIO | BIL2 | 105 | AMP0009857 | AMP0007548 | 4.21 | 2.01 | −0.41 | |
5A | STI1-GY | BIL2 | 88 | AMP0011577 | AMP0030240 | 3.81 | 10.50 | 0.08 | Hassouni et al. [25] |
5A | STI1-BIO | BIL2 | 3 | AMP0003832 | AMP0029058 | 2.75 | 6.20 | 0.20 | |
5A | STI2-GY | BIL2 | 88 | AMP0011577 | AMP0030240 | 7.84 | 13.70 | 0.09 | Hassouni et al. [25] |
5A | STI2-GY | BIL2 | 168 | AMP0008559 | AMP0030185 | 3.26 | 5.65 | 0.06 | Hassouni et al. [25] |
5A | STI1-BIO | BIL2 | 3 | AMP0003832 | AMP0029058 | 2.94 | 3.09 | 0.19 | |
5A | STI2-BIO | BIL2 | 87 | AMP0025208 | AMP0011577 | 4.14 | 1.12 | 0.08 | |
5A | STI2-BIO | BIL2 | 227 | AMP0001406 | AMP0015434 | 5.74 | 2.43 | −0.39 | |
5D | STI1-TKW | BIL1 | 7 | AMP0022256 | AMP0000398 | 4.39 | 14.08 | −0.12 | Wang et al. [26] |
5D | STI2-BIO | BIL2 | 204 | AMP0010296 | AMP0028613 | 2.95 | 3.10 | −0.29 | |
6D | STI1-GY | BIL1 | 53 | AMP0036794 | AMP0032738 | 3.03 | 8.67 | 0.07 | |
6D | STI2-GY | BIL2 | 92 | AMP0016445 | AMP0014713 | 3.90 | 6.84 | 0.07 | |
6D | STI2-TKW | BIL2 | 305 | AMP0003394 | AMP0027092 | 2.76 | 5.28 | −0.10 | Guan et al. [23] |
7D | STI2-TKW | BIL1 | 89 | AMP0019618 | AMP0017004 | 2.96 | 8.30 | −0.08 | Paliwal et al. [24] |
7D | STI2-BIO | BIL2 | 343 | AMP0018976 | AMP0002072 | 2.50 | 2.56 | −0.36 | |
7D | STI1-GY | BIL2 (BLUP) | 100 | AMP0021405 | AMP0025764 | 2.51 | 5.85 | 0.05 |
Environment | |||||||||
---|---|---|---|---|---|---|---|---|---|
Population | QTL | Chr 1 | PVE% 2 | Pos 3 | DN 4 | WA 5 | WM1 6 | WM2 7 | BIL1/BIL2 |
BIL1 | STI-TKW | 1A | 11.4–14.4 | 173 | √ | √ | |||
STI-TKW | 2B | 5.0–10.4 | 62–63 | √ | √ | ||||
BIL2 | TKW | 3A | 5.9–11.1 | 40–41 | √ | √ | √ | ||
STI-GY | 5A | 10.4–17.2 | 87–88 | √ | √ | ||||
Both BILs | STI-GY | 6D | 6.8–8.7 | 53–92 | √ |
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Ahmed, M.I.Y.; Kamal, N.M.; Gorafi, Y.S.A.; Abdalla, M.G.A.; Tahir, I.S.A.; Tsujimoto, H. Heat Stress-Tolerant Quantitative Trait Loci Identified Using Backcrossed Recombinant Inbred Lines Derived from Intra-Specifically Diverse Aegilops tauschii Accessions. Plants 2024, 13, 347. https://doi.org/10.3390/plants13030347
Ahmed MIY, Kamal NM, Gorafi YSA, Abdalla MGA, Tahir ISA, Tsujimoto H. Heat Stress-Tolerant Quantitative Trait Loci Identified Using Backcrossed Recombinant Inbred Lines Derived from Intra-Specifically Diverse Aegilops tauschii Accessions. Plants. 2024; 13(3):347. https://doi.org/10.3390/plants13030347
Chicago/Turabian StyleAhmed, Monir Idres Yahya, Nasrein Mohamed Kamal, Yasir Serag Alnor Gorafi, Modather Galal Abdeldaim Abdalla, Izzat Sidahmed Ali Tahir, and Hisashi Tsujimoto. 2024. "Heat Stress-Tolerant Quantitative Trait Loci Identified Using Backcrossed Recombinant Inbred Lines Derived from Intra-Specifically Diverse Aegilops tauschii Accessions" Plants 13, no. 3: 347. https://doi.org/10.3390/plants13030347