Extracellular Vesicles in the Oviduct: Progress, Challenges and Implications for the Reproductive Success
Abstract
:1. Introduction
2. Studies on Oviductal EVs
2.1. Analysis of oEVs Molecular Content
2.2. Oviductal EVs and Their Functional Effect on Gametes
2.3. Oviductal EVs and Their Functional Effect on Embryo(s)
3. Oviductal EVs: Current Knowledge about Their Molecular Content
3.1. Oviductal EVs and Proteins
3.2. Oviductal EVs and mRNA
3.3. Oviductal EVs and miRNA
3.4. Oviductal EVs and other Molecular Cargo
3.5. Regulation of the Dynamic and Complex Oviductal EVs Molecular Cargo
4. Oviductal EVs and Their Potential as Therapeutic Vectors
5. Challenges and Future Directions for oEVs Research
6. Concluding Remarks
Author Contributions
Funding
Conflicts of Interest
References
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Topic | Species | Year | Findings | Analyzed oEVS Content | Characterization Method | Exosomal Molecular Markers | Citation |
---|---|---|---|---|---|---|---|
Analysis of oEVs molecular content | Bovine | 2017 |
| Proteins | TEM and WB | HSP70 (WB) | Almiñana et al., 2017 Reproduction [24] |
Bovine | 2018 |
| proteins, mRNA and small ncRNA | TEM and WB | HSP70 and ANXA1 (WB) | Almiñana et al., 2018 BMC Genomics [37] | |
Murine | 2018 |
| miRNAs | TEM and WB | CD9 (WB) | Fereshteh et al., 2018 Scientific Reports [31] | |
oEVs and their functional effects on gametes (sperm and oocyte) | Murine | 2013 |
| protein PMCA4a | TEM and WB | HSC70 and CD9 (WB); CD9 (TEM) | Al-Dossary et al., 2013 PLOS One [22] |
Murine | 2015 |
| No | TEM and WB | PMCA4 (TEM) CD9 (WB) | Al-Dossary et al., 2015 J Biol Chem [58] | |
Avian | 2017 |
| No | WB | CD63 (WB) | Huang et al., 2017 Theriogenology [33] | |
Canine | 2017 |
| hsa-miR-30b, has-miR-375, cfa-miR-503 | NTA | _ | Lange-Consiglio et al., 2017 Reproduction [34] | |
Turtle | 2017 |
| No | TEM and immunostaining | CD63 (Immunostaining) | Waqas et al., 2017 J. Exp. Zool. [35] | |
Murine | 2018 |
| miRNAs | TEM and WB | CD9 (TEM) CD9 (WB) | Fereshteh et al., 2018 Sci Rep [31] | |
Human/Murine | 2018 |
| proteins (PMCA1-4) | TEM anand WB | PMCA4 (TEM) HSC70 (WB) | Bathala et al., 2018 Mol Hum Reprod [32] | |
oEVs and their functional effects on the embryo(s) | Bovine | 2016 |
| No | TEM, NTA and FC, WB | CD9 and CD63 (FC) CD9, ERM and TSG101 (WB) | Lopera-Vásquez et al., 2016 PLOS One [23] |
Bovine | 2017 |
| proteins | TEM and WB | HSP70 (WB) | Almiñana et al., 2017 Reproduction [24] | |
Bovine | 2017 |
| No | TEM, NTA and WB | CD9, ERM and TSG101 (WB) | Lopera-Vásquez et al., 2017 Reproduction [59] | |
Murine | 2019 |
| No | TEM; NTA; WB; BCA | CD9 and HSP70 (WB) | Qu et al., 2019 Reproduction, Fertility and Development [36] | |
Other oEVs functions | Murine | 2017 |
| mRNA (beta-actin, GAPDH and Vimentin) | TEM and WB | CD9 and CD81(WB) C81 (TEM) | Nakano et al., 2017 Biochem & Biophys Res Com [60] |
Bovine | Murine | Human | |||
---|---|---|---|---|---|
Protein Description | Symbol | Protein Description | Symbol | Protein Description | Symbol |
oviductal glycoprotein 1 | OVGP1 | Plasma membrane calcium-transporting ATPase 4 | PMCA4 | Plasma membrane calcium-transporting ATPase 4 | PMCA4 |
annexin A1 | ANXA1 | Plasma membrane calcium-transporting ATPase 1 | PMCA1 | Plasma membrane calcium-transporting ATPase 1 | PMCA1 |
tubulin, beta 2B class IIb | TUBB2B | Endothelial nitric oxide synthase | eNOS (NOS3) | Endothelial nitric oxide synthase | eNOS (NOS3) |
annexin A2 | ANXA2 | neuronal nitric oxide synthase | nNOS (NOS1) | neuronal nitric oxide synthase | nNOS (NOS1) |
annexin A4 | ANXA4 | calcium/calmodulin-dependent serine kinase | CASK | calcium/calmodulin-dependent serine kinase | CASK |
heat shock protein family A (Hsp70) member 8 | HSPA8 | heat shock protein family A (Hsp70) member 8 | HSPA8 (HSC70) | heat shock protein family A (Hsp70) member 8 | HSPA8 (HSC70) |
actin beta | ACTB | actin beta | ACTB | actin beta | ACTB |
CD109 molecule | CD109 | ||||
tubulin, alpha 3e | TUBA3E | ||||
annexin A5 | ANXA5 | ||||
heat shock 70kDa protein 1A | HSPA1A | ||||
heat shock protein 90 alpha family class A member 1 | HSP90AA1 | ||||
5’-nucleotidase ecto | NT5E | ||||
annexin A8-like 1 | ANXA8L1 | ||||
ezrin | EZR | ||||
clathrin heavy chain | CLTC | ||||
glyceraldehyde-3-phosphate dehydrogenase | GAPDH | ||||
stomatin | STOM | ||||
mesothelin | MSLN | ||||
major vault protein | MVP | ||||
annexin A11 | ANXA11 | ||||
ectonucleotide pyrophosphatase/phosphodiesterase 3 | ENPP3 | ||||
heat shock protein family B (small) member 1 | HSPB1 | ||||
clusterin | CLU | ||||
RAB5C, member RAS oncogene family | RAB5C |
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Almiñana, C.; Bauersachs, S. Extracellular Vesicles in the Oviduct: Progress, Challenges and Implications for the Reproductive Success. Bioengineering 2019, 6, 32. https://doi.org/10.3390/bioengineering6020032
Almiñana C, Bauersachs S. Extracellular Vesicles in the Oviduct: Progress, Challenges and Implications for the Reproductive Success. Bioengineering. 2019; 6(2):32. https://doi.org/10.3390/bioengineering6020032
Chicago/Turabian StyleAlmiñana, Carmen, and Stefan Bauersachs. 2019. "Extracellular Vesicles in the Oviduct: Progress, Challenges and Implications for the Reproductive Success" Bioengineering 6, no. 2: 32. https://doi.org/10.3390/bioengineering6020032