Role of Two Transcription Factors (TGA 1a and TGA 2.1) in the Mi-1-Mediated Resistance of Tomato to the Root-Knot Nematode Meloidogyne javanica
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Nematode Culture
2.3. VIGS Constructs
2.4. Growth of Agroclones and Agroinfiltration
2.5. Semiquantitative RT-PCR
2.6. Nematode Inoculation and Assessment of Infection Levels
3. Results
3.1. Assessment of the Effect of Agroinfiltration with the Empty Vector TRV
3.2. Silencing of Mi-1 Gene in Tomato Plants
3.3. Silencing of TGA 1a and TGA 2.1 Genes in Tomato Plants
4. Discussion
4.1. Effect of Agroinfiltration with the Empty Vector TRV and TRV-Mi
4.2. The Genes TGA 1a and TGA 2.1 in the Mi-1-Mediated Resistance to M. javanica
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Primer | Sequence | Fragment Length a | SGN b | Source |
---|---|---|---|---|---|
Mi-1 | Mi-RT-F | 5′-AGAGGAGGGAACGATCTTCAGA-3′ | 78 | U564276 | [32] |
Mi-RT-R | 5′-AAGCAAAGTTCAACCAAAATGCT-3′ | ||||
TGA 1a | TGA 1a-RT-F | 5′-GGGCTTACTCGCCTTGGGGG-3′ | 281 | U570984 | [49] |
TGA 1a-RT-R | 5′-CCAACGCGTTCAAAAGTTGCTCGG-3′ | ||||
TGA 2.1 | TGA 2.1-RT-F | 5′-CCAGACAATCAGCCCGTGCTCT-′ | 228 | U571820 | [49] |
TGA 2.1-RT-R | 5′-TTCTGCACTTCCACAAGCACATACA-3′ |
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Pascual, S.; Emiliozzi, M.; Nombela, G. Role of Two Transcription Factors (TGA 1a and TGA 2.1) in the Mi-1-Mediated Resistance of Tomato to the Root-Knot Nematode Meloidogyne javanica. Horticulturae 2024, 10, 134. https://doi.org/10.3390/horticulturae10020134
Pascual S, Emiliozzi M, Nombela G. Role of Two Transcription Factors (TGA 1a and TGA 2.1) in the Mi-1-Mediated Resistance of Tomato to the Root-Knot Nematode Meloidogyne javanica. Horticulturae. 2024; 10(2):134. https://doi.org/10.3390/horticulturae10020134
Chicago/Turabian StylePascual, Susana, Mariana Emiliozzi, and Gloria Nombela. 2024. "Role of Two Transcription Factors (TGA 1a and TGA 2.1) in the Mi-1-Mediated Resistance of Tomato to the Root-Knot Nematode Meloidogyne javanica" Horticulturae 10, no. 2: 134. https://doi.org/10.3390/horticulturae10020134