In Vitro Study of the Recruitment and Expansion of Mesenchymal Stem Cells at the Interface of a Cu-Doped PCL-Bioglass Scaffold
Abstract
:1. Introduction
2. Experimentals
2.1. Isolation and Culture of aBMSCs
2.2. Characterization of Isolated Cells
2.3. Scaffolds Materials and Preparation
2.4. Scaffold Characterization
2.4.1. Morphology Analysis
2.4.2. Mechanical Characterization
2.4.3. Hydrophilicity
2.4.4. FTIR Assessment
2.4.5. Mineralization Assay
2.4.6. Evaluation of Cu Ion Release
2.4.7. Antibacterial Property of the Scaffolds
2.5. Cell Attachment and Proliferation Assessment
2.6. SEM Analysis
2.7. Statistical Analysis
3. Result
3.1. Stem Cell Characterization
3.2. Membrane Characterization
3.3. FTIR Analysis
3.4. Mineralization Assay
3.5. Copper Release
3.6. Antibacterial Activity
3.7. Cell Attachment and Proliferation Assessment
3.8. Monitoring the Morphological Feature of Cells on Membranes
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Glass | Composition (wt.%) | ||||
---|---|---|---|---|---|
SiO2 | Na2O | CaO | P2O5 | CuO | |
Bioglass 45S5 | 45 | 24.5 | 24.5 | 6 | - |
Cu-bioglass | 45 | 24.5 | 23.5 | 6 | 1 |
Sample | Polymers | Weight Ratio (%) |
---|---|---|
1 | PCL | 100 |
2 | PCL/Gel | 50/50 |
3 | PCL/Gel/BG | 50/50.1% BG |
4 | PCL/Gel/CuBG | 50/50.1% CuBG |
Sample | Mean Fiber Diameter (nm) | First Layer Porosity (%) | Mean Pore Size (μm) | Contact Angle (°) |
---|---|---|---|---|
PCL | 622.72 ± 93 | 85.08 | 7.85 ± 3 | 105 ± 6 |
PCL/Gel | 407.56 ± 66 | 82.93 | 11.35 ± 5 | 28 ± 3 |
PCL/Gel/CuBG | 495 ± 22 | 82.82 | 12.82 ± 8 | 10 ± 2 |
PCL/Gel/BG | 486 ± 42 | 83.26 | 10.52 ± 6 | 14 ± 3 |
Sample | Tensile Strength (MPa) | Young’s Modulus (MPa) | Strain (%) |
---|---|---|---|
PCL | 3.12 ± 0.021 | 1.14 ± 0.01 | 176 ± 23 |
PCL/Gel | 2.36 ± 0.064 | 3.51 ± 0.03 | 85 ± 16 |
PCL/Gel/BG | 3.82 ± 0.036 | 3.95 ± 0.02 | 76 ± 12 |
PCL/Gel/CuBG | 3.54 ± 0.045 | 3.78 ± 0.02 | 81 ± 21 |
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Malekahmadi, B.; Esfahanian, V.; Ejeian, F.; Dastgurdi, M.E.; Agheb, M.; Kaveian, F.; Rafienia, M.; Nasr-Esfahani, M.H. In Vitro Study of the Recruitment and Expansion of Mesenchymal Stem Cells at the Interface of a Cu-Doped PCL-Bioglass Scaffold. Biomimetics 2022, 7, 19. https://doi.org/10.3390/biomimetics7010019
Malekahmadi B, Esfahanian V, Ejeian F, Dastgurdi ME, Agheb M, Kaveian F, Rafienia M, Nasr-Esfahani MH. In Vitro Study of the Recruitment and Expansion of Mesenchymal Stem Cells at the Interface of a Cu-Doped PCL-Bioglass Scaffold. Biomimetics. 2022; 7(1):19. https://doi.org/10.3390/biomimetics7010019
Chicago/Turabian StyleMalekahmadi, Behnaz, Vahid Esfahanian, Fatemeh Ejeian, Maziar Ebrahimi Dastgurdi, Maria Agheb, Faranak Kaveian, Mohammad Rafienia, and Mohammad Hossein Nasr-Esfahani. 2022. "In Vitro Study of the Recruitment and Expansion of Mesenchymal Stem Cells at the Interface of a Cu-Doped PCL-Bioglass Scaffold" Biomimetics 7, no. 1: 19. https://doi.org/10.3390/biomimetics7010019