Signal “Off-On” Biosensor Based on Fluorescence Resonance Energy Transfer (FRET) for Detection of Sorghum Mosaic Virus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Apparatus
2.2. Synthesis of CdTe QD
2.3. Synthesis of AuNP
2.4. Conjugation of SrMV Ab and CdTe QD
2.5. Conjugation of SrMV CP and AuNP
2.6. Determination of Quenching Efficiency
2.7. Quantitative Detection of Purified CP
3. Results
3.1. Morphological and Spectral Characterizations of CdTe QD and AuNP
3.2. Characterization of Bioconjugate
3.3. Principle Validation
3.4. FRET Efficiency
3.5. Optimization for the Biosensor
3.6. Feasibility Verification
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Sample | Added (μg) | Measured (μg) | Recovery (%) | RSD (n = 3, %) |
---|---|---|---|---|
1 | 1 | 1.07 ± 0.09 | 107.4 | 8.45 |
2 | 5 | 4.91 ± 0.34 | 98.2 | 6.92 |
3 | 10 | 9.77 ± 0.62 | 97.7 | 6.35 |
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Han, Z.; Du, P.; Wen, R.; Chen, B.; He, X. Signal “Off-On” Biosensor Based on Fluorescence Resonance Energy Transfer (FRET) for Detection of Sorghum Mosaic Virus. Analytica 2022, 3, 385-393. https://doi.org/10.3390/analytica3040026
Han Z, Du P, Wen R, Chen B, He X. Signal “Off-On” Biosensor Based on Fluorescence Resonance Energy Transfer (FRET) for Detection of Sorghum Mosaic Virus. Analytica. 2022; 3(4):385-393. https://doi.org/10.3390/analytica3040026
Chicago/Turabian StyleHan, Zhenlong, Pengfei Du, Ronghui Wen, Baoshan Chen, and **pu He. 2022. "Signal “Off-On” Biosensor Based on Fluorescence Resonance Energy Transfer (FRET) for Detection of Sorghum Mosaic Virus" Analytica 3, no. 4: 385-393. https://doi.org/10.3390/analytica3040026