Antagonism and Synergism Characterize the Interactions between Four North American Potato Virus Y Strains
Abstract
:1. Introduction
2. Materials and Methods
2.1. Virus Inoculations
2.2. Isolation of RNA and Reverse Transcription Quantitative PCR (RT-qPCR)
2.3. Determination of Relative Viral RNA Titer
3. Results
3.1. Antagonistic and Synergistic Interactions between PVY Strains in Potato
3.2. RT-qPCR Quantification of Viral RNA
3.3. Replication and Cell-To-Cell Movement of PVY Strains
3.4. Interactions between PVY Strains in Potato Tubers
3.5. Effect of Temperature on PVY Cell-to-Cell Movement in Tubers
3.6. Evidence of Virus Replication in Potato Tubers
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Scholthof, K.-B.G.; Adkins, S.; Czosnek, H.; Palukaitis, P.; Jacquot, E.; Hohn, T.; Hohn, B.; Saunders, K.; Candresse, T.; Ahlquist, P.; et al. Top 10 Plant Viruses in Molecular Plant Pathology. Mol. Plant Pathol. 2011, 12, 938–954. [Google Scholar] [CrossRef] [PubMed]
- Karasev, A.V.; Gray, S.M. Continuous and Emerging Challenges of Potato Virus Y in Potato. Annu. Rev. Phytopathol. 2013, 51, 571–586. [Google Scholar] [CrossRef] [PubMed]
- Gray, S.; De Boer, S.; Lorenzen, J.; Karasev, A.; Whitworth, J.; Nolte, P.; Singh, R.; Boucher, A.; Xu, H. Potato Virus Y: An Evolving Concern for Potato Crops in the United States and Canada. Plant Dis. 2010, 94, 1384–1397. [Google Scholar] [CrossRef]
- Karasev, A.V.; Hu, X.; Brown, C.J.; Kerlan, C.; Nikolaeva, O.V.; Crosslin, J.M.; Gray, S.M. Genetic Diversity of the Ordinary Strain of Potato Virus Y (PVY) and Origin of Recombinant PVY Strains. Phytopathology 2011, 101, 778–785. [Google Scholar] [CrossRef]
- Green, K.J.; Brown, C.J.; Gray, S.M.; Karasev, A.V. Phylogenetic Study of Recombinant Strains of Potato Virus Y. Virology 2017, 507, 40–52. [Google Scholar] [CrossRef] [PubMed]
- MacKenzie, T.D.B.; Nie, X.; Bisht, V.; Singh, M. Proliferation of Recombinant PVY Strains in Two Potato-Producing Regions of Canada, and Symptom Expression in 30 Important Potato Varieties with Different PVY Strains. Plant Dis. 2019, 103, 2221–2230. [Google Scholar] [CrossRef]
- Hu, X.; He, C.; ** and Marker-Assisted Selection Validation for PVY Resistance in Potato Breeding. Mol. Breed. 2014, 34, 267–271. [Google Scholar] [CrossRef] [PubMed]
- Syller, J. Facilitative and Antagonistic Interactions between Plant Viruses in Mixed Infections. Mol. Plant Pathol. 2012, 13, 204–216. [Google Scholar] [CrossRef] [PubMed]
- Syller, J.; Grupa, A. Antagonistic Within-Host Interactions between Plant Viruses: Molecular Basis and Impact on Viral and Host Fitness. Mol. Plant Pathol. 2016, 17, 769–782. [Google Scholar] [CrossRef] [PubMed]
- Tatineni, S.; Riethoven, J.-J.M.; Graybosch, R.A.; French, R.; Mitra, A. Dynamics of Small RNA Profiles of Virus and Host Origin in Wheat Cultivars Synergistically Infected by Wheat Streak Mosaic Virus and Triticum Mosaic Virus: Virus Infection Caused a Drastic Shift in the Endogenous Small RNA Profile. PLoS ONE 2014, 9, e111577. [Google Scholar] [CrossRef]
- Glais, L.; Faurez, F.; Tribodet, M.; Boulard, F.; Jacquot, E. The Amino Acid 419 in HC-Pro Is Involved in the Ability of PVY Isolate N605 to Induce Necrotic Symptoms on Potato Tubers. Virus Res. 2015, 208, 110–119. [Google Scholar] [CrossRef] [PubMed]
- Abrams, M. Environmental Grain, Organism Fitness, and Type Fitness. In Entangled Life; Springer: Dordrecht, The Netherlands, 2014; pp. 127–151. [Google Scholar] [CrossRef] [PubMed]
- Metcalf, C.J.E.; Birger, R.B.; Funk, S.; Kouyos, R.D.; Lloyd-Smith, J.O.; Jansen, V.a.A. Five Challenges in Evolution and Infectious Diseases. Epidemics 2015, 10, 40–44. [Google Scholar] [CrossRef] [PubMed]
- Barbour, J.D.; Grant, R.M. The Role of Viral Fitness in HIV Pathogenesis. Curr. HIV/AIDS Rep. 2005, 2, 29–34. [Google Scholar] [CrossRef] [PubMed]
- Domingo, E. Mechanisms of Viral Emergence. Vet. Res. 2010, 41, 38. [Google Scholar] [CrossRef] [PubMed]
- Wargo, A.R.; Kurath, G. Viral Fitness: Definitions, Measurement, and Current Insights. Curr. Opin. Virol. 2012, 2, 538–545. [Google Scholar] [CrossRef] [PubMed]
- Elena, S.F.; Lalić, J. Plant RNA Virus Fitness Predictability: Contribution of Genetic and Environmental Factors. Plant Pathol. 2013, 62, 10–18. [Google Scholar] [CrossRef]
- Milling, A.; Meng, F.; Denny, T.P.; Allen, C. Interactions with Hosts at Cool Temperatures, Not Cold Tolerance, Explain the Unique Epidemiology of Ralstonia Solanacearum Race 3 Biovar 2. Phytopathology 2009, 99, 1127–1134. [Google Scholar] [CrossRef]
- Fox, A.; Evans, F.; Browning, I. Direct Tuber Testing for Potato Y Potyvirus by Real-Time RT-PCR and ELISA: Reliable Options for Post-Harvest Testing?*. EPPO Bull. 2005, 35, 93–97. [Google Scholar] [CrossRef]
A/RT-PCR | |||
Target | Designation | Sequence (5’-3’) | Length (bp) |
* PVYNTN | NTN7350-F NTN8266-R | ACATCACCGATGAGCAGG GTACATACCCTCGATTAGCA | 918 |
* PVYO | O1962-F O2296-R | TCAACATTCTATCCACCAAC ACGTTTGAGTGTCATGGT | 335 |
PVYN:O | N:O1008-F N:O1703-R | GCACGTTCCAAGGTTACC TCGCTTAGCATGATATTCCCT | 695 |
** PVYN-Wi | N-Wi_YN5-1780-F N-Wi_YO3-2558-R | TCCGAATGGGACAAGAAAACTTG AGGCTCATCTAACAGCAACTGTC | 778 |
B/RT-qPCR *** | |||
Target | Designation | Sequence (5’-3’) | Length (bp) |
PVYNTN | NTNq-6515-F NTNq-6631-R | TCCGAGCTCCAGTGCAGAAT AAGTGCTGCCCGGTACATTG | 116 |
PVYO | Oq-4-F Oq-138-R | CGCAAAAACACTCATAAAAGCTCA TGGTTGGAAGTGATGAAATTGCT | 134 |
PVYN:O | NOq-6444-F NOq-6574-R | GGATATCATCCTCATCAAATGCCG TCGACGATGCATACTTCTCCTG | 130 |
PVYN-Wi | N-Wiq-35-F N-Wiq-156-R | TTCCTTGCAATTCTCTTAAACGGT ACGAACCGAAACAGATTGTTGAC | 121 |
All strains | Uni-q-9426-F Uni-q-9549-R | GTGGCAGGGTGATTTCGTCA AGAATCGCAACATCACCTAATCG | 123 |
1-alpha EF1α | EF1-F EF1-R | TGGAGGCACTCCCTGGTGACA TGTGGCAGTCGAGCACTGGT | 194 |
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Niraula, P.M.; Baldrich, P.; Cheema, J.A.; Cheema, H.A.; Gaiter, D.S.; Meyers, B.C.; Fondong, V.N. Antagonism and Synergism Characterize the Interactions between Four North American Potato Virus Y Strains. Int. J. Plant Biol. 2024, 15, 412-428. https://doi.org/10.3390/ijpb15020032
Niraula PM, Baldrich P, Cheema JA, Cheema HA, Gaiter DS, Meyers BC, Fondong VN. Antagonism and Synergism Characterize the Interactions between Four North American Potato Virus Y Strains. International Journal of Plant Biology. 2024; 15(2):412-428. https://doi.org/10.3390/ijpb15020032
Chicago/Turabian StyleNiraula, Prakash M., Patricia Baldrich, Junaid A. Cheema, Hashir A. Cheema, Dejah S. Gaiter, Blake C. Meyers, and Vincent N. Fondong. 2024. "Antagonism and Synergism Characterize the Interactions between Four North American Potato Virus Y Strains" International Journal of Plant Biology 15, no. 2: 412-428. https://doi.org/10.3390/ijpb15020032