Seasonal Freezing Drives Spatiotemporal Dynamics of Dissolved Organic Matter (DOM) and Microbial Communities in Reclaimed Water-Recharged River
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Area and Sample Collection
2.2. Detection of Water Quality Parameters
2.3. Optical Spectroscopy Measurements and Analysis
2.4. DNA Extraction and 16S rRNA Gene High-Throughput Sequencing
2.5. Pathogen Quantification Using High-Throughput Quantitative PCR (HT-qPCR)
2.6. Bioinformatics and Statistical Analysis
3. Results and Discussion
3.1. Variation in River Water Physicochemical Characteristics
3.2. Spectral Characteristics and Source Identification of DOM
3.3. BC Composition and Distribution Characteristics
3.4. Distribution Characteristics of Pathogenic Bacteria
3.5. Co-Occurrence Patterns among Bacterial Community and Their Interactions with Environmental Factors
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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T (°C) | pH | DO (mg/L) | NO3−-N (mg/L) | NO2−-N (mg/L) | NH4+-N (mg/L) | TN (mg/L) | TP (mg/L) | DOC (mg/L) | |
---|---|---|---|---|---|---|---|---|---|
Y3O | 5.2 | 5.47 | 6.66 | 1.22 | 0.021 | 0.02 | 1.23 | 0.05 | 6.078 |
Y4O | 4.7 | 5.45 | 7.03 | 1.54 | 0.029 | 0.72 | 1.92 | 0.08 | 9.708 |
Y5O | 13.3 | 5.51 | 6.08 | 3.76 | 0.052 | 2.96 | 3.95 | 0.17 | 11.57 |
Y6O | 7.8 | 5.47 | 6.51 | 3.35 | 0.033 | 1.88 | 3.67 | 0.05 | 10.66 |
Y7O | 8.8 | 5.48 | 6.19 | 3.12 | 0.05 | 0.66 | 3.37 | 0.13 | 8.92 |
Y8O | 8.2 | 7.48 | 5.87 | 2.45 | 0.063 | 1.13 | 3.00 | 0.17 | 9.003 |
Y10O | 12.2 | 8.11 | 5.44 | 2.14 | 0.115 | N | 2.53 | 0.28 | 8.086 |
Y11O | 15.1 | 7.36 | 5.74 | 3.52 | 0.09 | 1.63 | 3.88 | 0.32 | 9.908 |
Y12O | 13.9 | 7.89 | 5.84 | 2.96 | 0.167 | 3.25 | 3.43 | 0.10 | 12.61 |
Y13O | 13.2 | 8.02 | 5.32 | 1.23 | 0.116 | 4.6 | 1.57 | 1.25 | 24.63 |
Y3F | 3.4 | 7.47 | 6.16 | 1.54 | 0.008 | 0.01 | N | 0.08 | 8.629 |
Y5F | 8.2 | 7.42 | 6.47 | 3.77 | 0.019 | 0.02 | 3.54 | 0 | 11.74 |
Y6F | 3.5 | 7.57 | 6.79 | 3.46 | 0.014 | 0 | 3.62 | 0.03 | 10.78 |
Y7F | 2.8 | 7.61 | 6.97 | 3.05 | 0.023 | 0.37 | 3.35 | 0.23 | 10.32 |
Y8F | 1.9 | 7.67 | 6.83 | 2.33 | 0.031 | 0.52 | 2.66 | 0.32 | 10.04 |
Y11F | 9.8 | 7.53 | 5.55 | 3.55 | 0.127 | 0.97 | 2.74 | 0.20 | 15.58 |
Y13F | 2.7 | 7.69 | 6.81 | 2.14 | 0.055 | 1.64 | 2.37 | 0.15 | 12.55 |
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Zhao, J.; Huo, Y.; Zhang, Z.; Zhang, Y.; Hou, Z.; Fan, W.; Geng, Z.; Huo, M. Seasonal Freezing Drives Spatiotemporal Dynamics of Dissolved Organic Matter (DOM) and Microbial Communities in Reclaimed Water-Recharged River. Water 2024, 16, 906. https://doi.org/10.3390/w16060906
Zhao J, Huo Y, Zhang Z, Zhang Y, Hou Z, Fan W, Geng Z, Huo M. Seasonal Freezing Drives Spatiotemporal Dynamics of Dissolved Organic Matter (DOM) and Microbial Communities in Reclaimed Water-Recharged River. Water. 2024; 16(6):906. https://doi.org/10.3390/w16060906
Chicago/Turabian StyleZhao, Jiaqi, Yang Huo, Zhiruo Zhang, Ying Zhang, Zhenlai Hou, Wei Fan, Zhi Geng, and Mingxin Huo. 2024. "Seasonal Freezing Drives Spatiotemporal Dynamics of Dissolved Organic Matter (DOM) and Microbial Communities in Reclaimed Water-Recharged River" Water 16, no. 6: 906. https://doi.org/10.3390/w16060906