Determination of the Binding Epitope of an Anti-Mouse CCR9 Monoclonal Antibody (C9Mab-24) Using the 1× Alanine and 2× Alanine-Substitution Method
Abstract
:1. Introduction
2. Materials and Methods
2.1. Development of C9Mab-24
2.2. ELISA
3. Results
3.1. Epitope Determination Using 1× Ala-substituted mCCR9 Peptides
3.2. Epitope Determination Using 2× Ala-substituted mCCR9 Peptides
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Peptides | Sequences | C9Mab-24 |
---|---|---|
WT | MMPTELTSLIPGMFDDFSY | ++ |
M1A | AMPTELTSLIPGMFDDFSY | ++ |
M2A | MAPTELTSLIPGMFDDFSY | ++ |
P3A | MMATELTSLIPGMFDDFSY | ++ |
T4A | MMPAELTSLIPGMFDDFSY | ++ |
E5A | MMPTALTSLIPGMFDDFSY | ++ |
L6A | MMPTEATSLIPGMFDDFSY | ++ |
T7A | MMPTELASLIPGMFDDFSY | ++ |
S8A | MMPTELTALIPGMFDDFSY | ++ |
L9A | MMPTELTSAIPGMFDDFSY | ++ |
I10A | MMPTELTSLAPGMFDDFSY | ++ |
P11A | MMPTELTSLIAGMFDDFSY | ++ |
G12A | MMPTELTSLIPAMFDDFSY | ++ |
M13A | MMPTELTSLIPGAFDDFSY | ++ |
F14A | MMPTELTSLIPGMADDFSY | – |
D15A | MMPTELTSLIPGMFADFSY | ++ |
D16A | MMPTELTSLIPGMFDAFSY | ++ |
F17A | MMPTELTSLIPGMFDDASY | – |
S18A | MMPTELTSLIPGMFDDFAY | ++ |
Y19A | MMPTELTSLIPGMFDDFSA | ++ |
Peptides | Sequences | C9Mab-24 |
---|---|---|
WT | MMPTELTSLIPGMFDDFSY | ++ |
M1A–M2A | AAPTELTSLIPGMFDDFSY | ++ |
M2A–P3A | MAATELTSLIPGMFDDFSY | ++ |
P3A–T4A | MMAAELTSLIPGMFDDFSY | ++ |
T4A–E5A | MMPAALTSLIPGMFDDFSY | ++ |
E5A–L6A | MMPTAATSLIPGMFDDFSY | ++ |
L6A–T7A | MMPTEAASLIPGMFDDFSY | ++ |
T7A–S8A | MMPTELAALIPGMFDDFSY | ++ |
S8A–L9A | MMPTELTAAIPGMFDDFSY | ++ |
L9A–I10A | MMPTELTSAAPGMFDDFSY | ++ |
I10A–P11A | MMPTELTSLAAGMFDDFSY | ++ |
P11A–G12A | MMPTELTSLIAAMFDDFSY | ++ |
G12A–M13A | MMPTELTSLIPAAFDDFSY | ++ |
M13A–F14A | MMPTELTSLIPGAADDFSY | – |
F14A–D15A | MMPTELTSLIPGMAADFSY | – |
D15A–D16A | MMPTELTSLIPGMFAAFSY | ++ |
D16A–F17A | MMPTELTSLIPGMFDAASY | – |
F17A–S18A | MMPTELTSLIPGMFDDAAY | – |
S18A–Y19A | MMPTELTSLIPGMFDDFAA | ++ |
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Kobayashi, H.; Asano, T.; Tanaka, T.; Suzuki, H.; Kaneko, M.K.; Kato, Y. Determination of the Binding Epitope of an Anti-Mouse CCR9 Monoclonal Antibody (C9Mab-24) Using the 1× Alanine and 2× Alanine-Substitution Method. Antibodies 2023, 12, 11. https://doi.org/10.3390/antib12010011
Kobayashi H, Asano T, Tanaka T, Suzuki H, Kaneko MK, Kato Y. Determination of the Binding Epitope of an Anti-Mouse CCR9 Monoclonal Antibody (C9Mab-24) Using the 1× Alanine and 2× Alanine-Substitution Method. Antibodies. 2023; 12(1):11. https://doi.org/10.3390/antib12010011
Chicago/Turabian StyleKobayashi, Hiyori, Teizo Asano, Tomohiro Tanaka, Hiroyuki Suzuki, Mika K. Kaneko, and Yukinari Kato. 2023. "Determination of the Binding Epitope of an Anti-Mouse CCR9 Monoclonal Antibody (C9Mab-24) Using the 1× Alanine and 2× Alanine-Substitution Method" Antibodies 12, no. 1: 11. https://doi.org/10.3390/antib12010011