Higher Prevalence of the Periodontal Pathogen Selenomonas noxia among Pediatric and Adult Patients May Be Associated with Overweight and Obesity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Approval
2.2. Original Sample Collection
2.3. DNA Isolation
2.4. qPCR Screening
- Positive controls
(Human) GAPDH forward, | 5′ATCTTCCAGGAGCGAGATCC-3′ |
(Human) GAPDH reverse, | 5′ACCACTGACACGTTGGCAGT-3′ |
(Human) Beta actin forward, | 5′-GTGGGGTCCTGTGGTGTG-3′ |
(Human) Beta actin reverse, | 5′-GAAGGGGACAGGCAGTGA-3′ |
(Bacterial) 16S rRNA forward, | 5′-ACGCGTCGACAGAGTTTGATCCTGGCT-3′ |
(Bacterial) 16S rRNA reverse, | 5′-GGGACTACCAGGGTATCTAAT-3′ |
- Selenomonas noxia
SN forward primer: | 5′-TCTGGGCTACACACGTACTACAATG-3′ |
SN reverse primer: | 5′-GCCTGCAATCCGAACTGAGA-3′ |
2.5. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Demographic | Study Sample (n = 122) | UNLV-SDM Clinic Population | Statistical Analysis |
---|---|---|---|
Sex | |||
Male | 53.2% n = 65/122 | 50.9% | X2 = 0.160, d.f. = 1 p = 0.6891 |
Female | 46.7% n = 57/122 | 49.1% | |
Race or Ethnicity | |||
White/non-minority | 36.1% n = 44/122 | 34.6% | X2 = 0.044, d.f. = 1 p = 0.8339 |
Minority/non-White | 63.9% n = 78/122 | 65.4% | |
Hispanic/Latino | 33.6% n = 41/122 | 52.4% | |
Black/Afr. Amer. | 17.2% n = 21/122 | 12.2% | |
Asian/Pac Islander | 4.1% n = 5/122 | 3.8% | |
Other | 7.4% n = 9/122 | 3.0% | |
Age | |||
Average | 25.08 years 14.09 years (Pediatric) 33.51 years (Adult) | 25.67 years 9.04 years (Pediatric) 42.3 years (Adult) | Two tailed t-test p = 0.778 |
Range | 7–17 years (Peds) 18–69 years (Adult) | 0–17 years (Peds) 18–89 years (Adult) |
Demographic | Study Sample (n = 122) | UNLV-SDM Clinic Population | Statistical Analysis |
---|---|---|---|
Body Mass Index | |||
Normal BMI 18–24 | 22.9% n = 28/122 | 25.9% | X2 = 2.028, d.f. = 2 p = 0.3627 |
Overweight BMI 25–29 | 21.3% n = 26/122 | 25.4% | |
Obese BMI 30 and over | 55.7% n = 68/94 | 48.7% | |
Average (Overweight/Obese) | 29.8 overall 32.51 above normal | 31.28 overall |
Demographic | S. noxia Positive | S noxia Negative | Statistical Analysis |
---|---|---|---|
Sex | |||
Males | 55.8% (n = 24/43) | 48.1% (n = 38/79) | X2 = 2.564, d.f. = 1 p = 0.1093 |
Females | 44.2% (n = 19/43) | 51.9% (n = 41/79) | |
Race/Ethnicity | |||
White/Caucasian | 39.5% (n = 17/43) | 34.2% (n = 27/79) | X2 = 1.099, d.f. = 1 p= 0.2945 |
non-White/Minority | 60.5% (n = 26/43) | 65.8% (n = 52/79) | |
Age | |||
Average | 26.01 years | 24.12 years | Two-tailed t-test p = 0.661 |
Age 7–17 | 51.2% (n = 22/43) | 39.2% (n = 31/79) | X2 = 6.053, d.f. = 1 p = 0.0139 |
Age 18–69 | 48.8% (n = 21/43) | 60.6% (n = 48/79) |
Demographic | S. noxia Positive | S. noxia Negative | Statistical Analysis |
---|---|---|---|
BMI | |||
Normal weight BMI 18–24 | 21.42% (n = 6/28) | 78.57% (n = 22/28) | X2 = 13.619, d.f. = 2 p = 0.0002 |
Overweight BMI 25–29 | 46.1% (n = 12/26) | 53.9% (n = 14/26) | OR = 3.67 95% CI [1.33, 10.08] p = 0.005895 |
Obese BMI 30–over | 36.8% (n = 25/68) | 63.2% (n = 43/68) | RR = 2.33 95% CI [1.108, 4.91} p = 0.0258 |
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Williams, A.; Porter, J.; Kingsley, K.; Howard, K.M. Higher Prevalence of the Periodontal Pathogen Selenomonas noxia among Pediatric and Adult Patients May Be Associated with Overweight and Obesity. Pathogens 2024, 13, 338. https://doi.org/10.3390/pathogens13040338
Williams A, Porter J, Kingsley K, Howard KM. Higher Prevalence of the Periodontal Pathogen Selenomonas noxia among Pediatric and Adult Patients May Be Associated with Overweight and Obesity. Pathogens. 2024; 13(4):338. https://doi.org/10.3390/pathogens13040338
Chicago/Turabian StyleWilliams, Austin, Jace Porter, Karl Kingsley, and Katherine M. Howard. 2024. "Higher Prevalence of the Periodontal Pathogen Selenomonas noxia among Pediatric and Adult Patients May Be Associated with Overweight and Obesity" Pathogens 13, no. 4: 338. https://doi.org/10.3390/pathogens13040338