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Article

Application of MALDI-TOF MS to Identify and Detect Antimicrobial-Resistant Streptococcus uberis Associated with Bovine Mastitis

by
Tingrui Zhang
1,2,
Duangporn Pichpol
3 and
Sukolrat Boonyayatra
1,4,*
1
Department of Food Animal Clinic, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand
2
Department of Veterinary Public Health, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 100191, China
3
Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand
4
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Long Island University, Brookville, NY 11548, USA
*
Author to whom correspondence should be addressed.
Microorganisms 2024, 12(7), 1332; https://doi.org/10.3390/microorganisms12071332
Submission received: 8 May 2024 / Revised: 26 June 2024 / Accepted: 27 June 2024 / Published: 29 June 2024
(This article belongs to the Special Issue Research on Infections and Veterinary Medicine)

Abstract

Streptococcus uberis is a common bovine mastitis pathogen in dairy cattle. The rapid identification and characterization of antimicrobial resistance (AMR) in S. uberis plays an important role in its diagnosis, treatment, and prevention. In this study, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to identify S. uberis and screen for potential AMR biomarkers. Streptococcus uberis strains (n = 220) associated with bovine mastitis in northern Thailand were identified using the conventional microbiological methods and compared with the results obtained from MALDI-TOF MS. Streptococcus uberis isolates were also examined for antimicrobial susceptibility using a microdilution method. Principal component analysis (PCA) and the Mann–Whitney U test were used to analyze the MALDI-TOF mass spectrum of S. uberis and determine the difference between antimicrobial-resistant and -susceptible strains. Using MALDI-TOF MS, 73.18% (161/220) of the sampled isolates were identified as S. uberis, which conformed to the identifications obtained using conventional microbiological methods and PCR. Using PCR, antimicrobial-resistant strains could not be distinguished from antimicrobial-susceptible strains for all three antimicrobial agents, i.e., tetracycline, ceftiofur, and erythromycin. The detection of spectral peaks at 7531.20 m/z and 6804.74 m/z was statistically different between tetracycline- and erythromycin-resistant and susceptible strains, respectively. This study demonstrates a proteomic approach for the diagnosis of bovine mastitis and potentially for the surveillance of AMR among bovine mastitis pathogens.
Keywords: bovine mastitis; Streptococcus uberis; MALDI-TOF MS; antimicrobial resistance bovine mastitis; Streptococcus uberis; MALDI-TOF MS; antimicrobial resistance

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MDPI and ACS Style

Zhang, T.; Pichpol, D.; Boonyayatra, S. Application of MALDI-TOF MS to Identify and Detect Antimicrobial-Resistant Streptococcus uberis Associated with Bovine Mastitis. Microorganisms 2024, 12, 1332. https://doi.org/10.3390/microorganisms12071332

AMA Style

Zhang T, Pichpol D, Boonyayatra S. Application of MALDI-TOF MS to Identify and Detect Antimicrobial-Resistant Streptococcus uberis Associated with Bovine Mastitis. Microorganisms. 2024; 12(7):1332. https://doi.org/10.3390/microorganisms12071332

Chicago/Turabian Style

Zhang, Tingrui, Duangporn Pichpol, and Sukolrat Boonyayatra. 2024. "Application of MALDI-TOF MS to Identify and Detect Antimicrobial-Resistant Streptococcus uberis Associated with Bovine Mastitis" Microorganisms 12, no. 7: 1332. https://doi.org/10.3390/microorganisms12071332

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