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Advances in Molecular and Cellular Imaging, Microscopy, and Biomedical Spectroscopy
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Advances in Molecular and Cellular Imaging, Microscopy, and Biomedical Spectroscopy

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biophysics".

Deadline for manuscript submissions: closed (30 June 2024) | Viewed by 3970

Special Issue Editor

Dr. Yusuke Oshima

E-Mail Website
Guest Editor
Faculty of Engineering, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan
Interests: fluorescent imaging; raman spectroscopy; nonlinear microscopy; optical biopsy; cancer metastasis; cancer diagnosis; osteoporosis; cartilage degeneration and regeneration
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Optical imaging and spectroscopic techniques are promising tools used to visualize molecular dynamics in living cells, organoids, and tissues in the fields of developmental biology, tissue engineering, immune response, tumorigenesis, and regenerative medicine. Recent advances in laser optics, imaging and microscopic technologies, and molecular probes have drastically improved sensitivity, specificity, time and spatial resolution, penetration dept, etc., for molecular and cellular imaging, microscopy, and biomedical spectroscopy. This Special Issue encourages the publication of methodologies that help to elucidate molecular mechanisms, cellular functions, and tissue morphologies in biological systems with and/or without labeling. Various research themes and topics (including nonlinear optical imaging; multiphoton fluorescence; SHG, THG, CARS, and SRS microscopy; photoacoustic imaging; NIR imaging and spectroscopy; and spontaneous Raman spectroscopy) are very welcome, in combination with modality and molecular sciences.

Dr. Yusuke Oshima
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at mdpi.longhoe.net by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (4 papers)

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Research

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11 pages, 1707 KiB  
Article
Cryptic Extensibility in von Willebrand Factor Revealed by Molecular Nanodissection
by Mária Csilla Csányi, Dominik Sziklai, Tímea Feller, Jolán Hársfalvi and Miklós Kellermayer
Int. J. Mol. Sci. 2024, 25(13), 7296; https://doi.org/10.3390/ijms25137296 - 2 Jul 2024
Viewed by 315
Abstract
Von Willebrand factor (VWF) is a multimer with a variable number of protomers, each of which is a head-to-head dimer of two multi-domain monomers. VWF responds to shear through the unfolding and extension of distinct domains, thereby mediating platelet adhesion and aggregation to [...] Read more.
Von Willebrand factor (VWF) is a multimer with a variable number of protomers, each of which is a head-to-head dimer of two multi-domain monomers. VWF responds to shear through the unfolding and extension of distinct domains, thereby mediating platelet adhesion and aggregation to the injured blood vessel wall. VWF's C1-6 segment uncoils and then the A2 domain unfolds and extends in a hierarchical and sequential manner. However, it is unclear whether there is any reservoir of further extensibility. Here, we explored the presence of cryptic extensibility in VWF by nanodissecting individual, pre-stretched multimers with atomic force microscopy (AFM). The AFM cantilever tip was pressed into the surface and moved in a direction perpendicular to the VWF axis. It was possible to pull out protein loops from VWF, which resulted in a mean contour length gain of 217 nm. In some cases, the loop became cleaved, and a gap was present along the contour. Frequently, small nodules appeared in the loops, indicating that parts of the nanodissected VWF segment remained folded. After analyzing the nodal structure, we conclude that the cryptic extensibility lies within the C1-6 and A1-3 regions. Cryptic extensibility may play a role in maintaining VWF’s functionality in extreme shear conditions. Full article
(This article belongs to the Special Issue Advances in Molecular and Cellular Imaging, Microscopy, and Biomedical Spectroscopy)
13 pages, 1790 KiB  
Article
Pure-Shift-Based Proton Magnetic Resonance Spectroscopy for High-Resolution Studies of Biological Samples
by Haolin Zhan, Yulei Chen, Yin** Cui, Yunsong Zeng, ** Lin, Yuqing Huang and Zhong Chen
Int. J. Mol. Sci. 2024, 25(9), 4698; https://doi.org/10.3390/ijms25094698 - 25 Apr 2024
Viewed by 642
Abstract
Proton magnetic resonance spectroscopy (1H MRS) presents a powerful tool for revealing molecular-level metabolite information, complementary to the anatomical insight delivered by magnetic resonance imaging (MRI), thus playing a significant role in in vivo/in vitro biological studies. However, its further applications [...] Read more.
Proton magnetic resonance spectroscopy (1H MRS) presents a powerful tool for revealing molecular-level metabolite information, complementary to the anatomical insight delivered by magnetic resonance imaging (MRI), thus playing a significant role in in vivo/in vitro biological studies. However, its further applications are generally confined by spectral congestion caused by numerous biological metabolites contained within the limited proton frequency range. Herein, we propose a pure-shift-based 1H localized MRS method as a proof of concept for high-resolution studies of biological samples. Benefitting from the spectral simplification from multiplets to singlet peaks, this method addresses the challenge of spectral congestion encountered in conventional MRS experiments and facilitates metabolite analysis from crowded NMR resonances. The performance of the proposed pure-shift 1H MRS method is demonstrated on different kinds of samples, including brain metabolite phantom and in vitro biological samples of intact pig brain tissue and grape tissue, using a 7.0 T animal MRI scanner. This proposed MRS method is readily implemented in common commercial NMR/MRI instruments because of its generally adopted pulse-sequence modules. Therefore, this study takes a meaningful step for MRS studies toward potential applications in metabolite analysis and disease diagnosis. Full article
(This article belongs to the Special Issue Advances in Molecular and Cellular Imaging, Microscopy, and Biomedical Spectroscopy)
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14 pages, 1613 KiB  
Article
Establishing Monoclonal Gammopathy of Undetermined Significance as an Independent Pre-Disease State of Multiple Myeloma Using Raman Spectroscopy, Dynamical Network Biomarker Theory, and Energy Landscape Analysis
by Shota Yonezawa, Takayuki Haruki, Keiichi Koizumi, Akinori Taketani, Yusuke Oshima, Makito Oku, Akinori Wada, Tsutomu Sato, Naoki Masuda, Jun Tahara, Noritaka Fujisawa, Shota Koshiyama, Makoto Kadowaki, Isao Kitajima and Shigeru Saito
Int. J. Mol. Sci. 2024, 25(3), 1570; https://doi.org/10.3390/ijms25031570 - 26 Jan 2024
Viewed by 1122
Abstract
Multiple myeloma (MM) is a cancer of plasma cells. Normal (NL) cells are considered to pass through a precancerous state, such as monoclonal gammopathy of undetermined significance (MGUS), before transitioning to MM. In the present study, we acquired Raman spectra at three stages—834 [...] Read more.
Multiple myeloma (MM) is a cancer of plasma cells. Normal (NL) cells are considered to pass through a precancerous state, such as monoclonal gammopathy of undetermined significance (MGUS), before transitioning to MM. In the present study, we acquired Raman spectra at three stages—834 NL, 711 MGUS, and 970 MM spectra—and applied the dynamical network biomarker (DNB) theory to these spectra. The DNB analysis identified MGUS as the unstable pre-disease state of MM and extracted Raman shifts at 1149 and 1527–1530 cm−1 as DNB variables. The distribution of DNB scores for each patient showed a significant difference between the mean values for MGUS and MM patients. Furthermore, an energy landscape (EL) analysis showed that the NL and MM stages were likely to become stable states. Raman spectroscopy, the DNB theory, and, complementarily, the EL analysis will be applicable to the identification of the pre-disease state in clinical samples. Full article
(This article belongs to the Special Issue Advances in Molecular and Cellular Imaging, Microscopy, and Biomedical Spectroscopy)
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Review

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19 pages, 2993 KiB  
Review
Pushing the Resolution Limit of Stimulated Emission Depletion Optical Nanoscopy
by Sejoo Jeong, Dongbin Koh, Eunha Gwak, Chinmaya V. Srambickal, Daeha Seo, Jerker Widengren and Jong-Chan Lee
Int. J. Mol. Sci. 2024, 25(1), 26; https://doi.org/10.3390/ijms25010026 - 19 Dec 2023
Viewed by 1360
Abstract
Optical nanoscopy, also known as super-resolution optical microscopy, has provided scientists with the means to surpass the diffraction limit of light microscopy and attain new insights into nanoscopic structures and processes that were previously inaccessible. In recent decades, numerous studies have endeavored to [...] Read more.
Optical nanoscopy, also known as super-resolution optical microscopy, has provided scientists with the means to surpass the diffraction limit of light microscopy and attain new insights into nanoscopic structures and processes that were previously inaccessible. In recent decades, numerous studies have endeavored to enhance super-resolution microscopy in terms of its spatial (lateral) resolution, axial resolution, and temporal resolution. In this review, we discuss recent efforts to push the resolution limit of stimulated emission depletion (STED) optical nanoscopy across multiple dimensions, including lateral resolution, axial resolution, temporal resolution, and labeling precision. We introduce promising techniques and methodologies building on the STED concept that have emerged in the field, such as MINSTED, isotropic STED, and event-triggered STED, and evaluate their respective strengths and limitations. Moreover, we discuss trade-off relationships that exist in far-field optical microscopy and how they come about in STED optical nanoscopy. By examining the latest developments addressing these aspects, we aim to provide an updated overview of the current state of STED nanoscopy and its potential for future research. Full article
(This article belongs to the Special Issue Advances in Molecular and Cellular Imaging, Microscopy, and Biomedical Spectroscopy)
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