Understanding of the Microbiome at the Genome Level

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Systems Microbiology".

Deadline for manuscript submissions: 31 August 2024 | Viewed by 1158

Special Issue Editors


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Guest Editor
School of Biological Sciences and Technology, Yangzhou University, Yangzhou, China
Interests: next-generation sequencing data analysis; microbiome; the interaction between the microbiome and the host and the interaction between the microbiome and specific microorganisms (such as pathogenic bacteria)
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
College of Animal Science and Technology, Yangzhou University, Yangzhou, China
Interests: next-generation sequencing data analysis; microbiome; the interaction between the microbiome and the host and the interaction between the microbiome and specific microorganisms (such as pathogenic bacteria)

Special Issue Information

Dear Colleagues,

A vast number of microorganisms populate host-associated environments, collectively forming microbiomes. These microbes can be broadly categorized as mutualists, pathogens, or commensals based on their interactions with the host. Mutualists play a crucial role in benefiting hosts, contributing to functions like nutrient absorption and resistance against pathogen invasion. Conversely, pathogens can induce specific diseases upon colonization. Notably, the invasion of certain pathogens can disrupt the balance of the microbiome, facilitating their infection process. Conversely, specific mutualists can mitigate and prevent pathogen invasion through direct antagonistic actions, modulation of host immune responses, and other mechanisms. A comprehensive understanding of how beneficial and pathogenic microbes interact with the host and other microorganisms is essential for develo** microbiome manipulation strategies to enhance host health. As the Guest Editors of this Special Issue, we encourage the scientific community to submit contributions (original research articles, review articles, and short communications) in the following (but not exclusive) areas:

  1. Genomic research on microorganisms isolated (or recovered from metagenomic data) from the microbiomes.
  2. Genome-resolved inter-microbial interactions inside the microbiome
  3. Roles of beneficial- or pathogenicity-associated genes in the interactions of microbes with the microbiome
  4. The role of mycobiota and their interaction with bacteria
  5. The role of gut microbiota in stress (weaning stress, nutritional stress, heat stress, oxidative stress and etc.)

Prof. Dr. Yunzeng Zhang
Dr. Haoyu Liu
Guest Editors

Manuscript Submission Information

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Keywords

  • beneficial
  • pathogenic
  • microbe
  • microbiome
  • interaction
  • genome
  • transcriptome
  • mycobiota
  • stress

Published Papers (2 papers)

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Research

10 pages, 832 KiB  
Article
First Insight into the Whole Genome Sequencing Whole Variations in Mycobacterium bovis from Cattle in Morocco
by Mohammed Khoulane, Siham Fellahi, Slimane Khayi, Mohammed Bouslikhane, Hassan Lakhdissi and Jaouad Berrada
Microorganisms 2024, 12(7), 1316; https://doi.org/10.3390/microorganisms12071316 - 27 Jun 2024
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Abstract
Six cattle heads which tested positive against bovine tuberculosis (bTB) in Morocco were investigated to confirm the disease and to determine the source(s) of infection. Polymerase Chain Reaction (PCR) was directly performed on tissue samples collected from slaughtered animals. All investigated animals tested [...] Read more.
Six cattle heads which tested positive against bovine tuberculosis (bTB) in Morocco were investigated to confirm the disease and to determine the source(s) of infection. Polymerase Chain Reaction (PCR) was directly performed on tissue samples collected from slaughtered animals. All investigated animals tested positive to PCR for the Mycobacterium bovis sub-type. Bacteriological isolation was conducted according to the technique recommended by WOAH for the cultivation of the Mycobacterium tuberculosis Complex (MBTC). Whole genome sequencing (WGS) was carried out on six mycobacterial isolates and the phylogenic tree was constructed. The six Moroccan isolates fit with clades II, III, IV, V and VII and were confirmed to belong to the clonal complexes Eu2, Unknown 2 and 7 as well as to sublineages La1.7.1, La1.2 and La1.8.2. The significant Single Nucleotide Polymorphism (SNPs) ranged from 84 to 117 between the isolates and the reference M. bovis strain and from 17 to 212 between the six isolates. Considering the high resolution of WGS, these results suggests that the source of infection of the bTB could be linked to imported animals as five of the investigated reactor animals were imported a few months prior. WGS can be a useful component to the Moroccan strategy to control bTB. Full article
(This article belongs to the Special Issue Understanding of the Microbiome at the Genome Level)
16 pages, 2553 KiB  
Article
Bacillus velezensis YXDHD1-7 Prevents Early Blight Disease by Promoting Growth and Enhancing Defense Enzyme Activities in Tomato Plants
by Wangxi Li, Lili Sun, Hangtao Wu, Wenjie Gu, Yusheng Lu, Chong Liu, Jiexin Zhang, Wanling Li, Changmin Zhou, Haoyang Geng, Yaying Li, Huanlong Peng, Chaohong Shi, Dan Wang and Guixiang Peng
Microorganisms 2024, 12(5), 921; https://doi.org/10.3390/microorganisms12050921 - 30 Apr 2024
Cited by 1 | Viewed by 706
Abstract
Bacillus velezensis is well known as a plant growth-promoting rhizobacteria (PGPR) and biocontrol agent. Nevertheless, there are very few reports on the study of B. velezensis on tomato early blight, especially the biocontrol effects among different inoculation concentrations. In this study, an IAA-producing [...] Read more.
Bacillus velezensis is well known as a plant growth-promoting rhizobacteria (PGPR) and biocontrol agent. Nevertheless, there are very few reports on the study of B. velezensis on tomato early blight, especially the biocontrol effects among different inoculation concentrations. In this study, an IAA-producing strain, Bacillus velezensis YXDHD1-7 was isolated from the tomato rhizosphere soil, which had the strongest inhibitory effect against Alternaria solani. Inoculation with bacterial suspensions of this strain promoted the growth of tomato seedlings effectively. Furthermore, inoculations at 106, 107, and 108 cfu/mL resulted in control efficacies of 100%, 83.15%, and 69.90%, respectively. Genome sequencing showed that it possesses 22 gene clusters associated with the synthesis of antimicrobial metabolites and genes that are involved in the production of IAA. Furthermore, it may be able to produce spermidine and volatile compounds that also enhance plant growth and defense responses. Our results suggest that strain YXDHD1-7 prevents early blight disease by promoting growth and enhancing the defense enzyme activities in tomato plants. This strain is a promising candidate for an excellent microbial inoculant that can be used to enhance tomato production. Full article
(This article belongs to the Special Issue Understanding of the Microbiome at the Genome Level)
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