Advances in Research on Waterborne Pathogens

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Environmental Microbiology".

Deadline for manuscript submissions: 31 October 2024 | Viewed by 477

Special Issue Editor


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Guest Editor
Biology Department, Lamar University, Beaumont, TX 77710, USA
Interests: molecular pathogenesis of parasitic protozoan species; host-parasite interaction and signaling

Special Issue Information

Dear Colleagues,

Research on waterborne pathogens has become increasingly critical in addressing public health concerns associated with water contamination. The study of waterborne pathogens encompasses a wide range of interdisciplinary fields, including microbiology, environmental science, public health, and epidemiology. Understanding the transmission, survival, and control of waterborne pathogens is essential for safeguarding water quality and human health.

For this Special Issue, we welcome contributions that address the key areas of research mentioned above and provide valuable insights into the dynamics and management of waterborne pathogens. Original articles, review articles and short communications are welcome. Topics include but are not limited to the following:

  • Pathogen Detection and Identification: Advancements in molecular biology and genomics have revolutionized the detection and characterization of waterborne pathogens, enabling more accurate and rapid identification of microbial contaminants.
  • Pathogen Persistence and Adaptation: Exploring the survival mechanisms and adaptive strategies of waterborne pathogens in diverse environmental conditions are crucial for predicting and mitigating their impact on water sources.
  • Emerging Pathogens and Antimicrobial Resistance: Investigating the emergence of new waterborne pathogens and the spread of antimicrobial resistance genes in aquatic environments is vital for proactive public health interventions.
  • Future research in this field is expected to focus on the development of innovative water treatment technologies, the impact of climate change on waterborne diseases, and the implementation of advanced surveillance systems for early detection of waterborne outbreaks.

Dr. Ashwini S. Kucknoor
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at mdpi.longhoe.net by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • waterborne pathogens
  • pathogen detection
  • antimicrobial resistance
  • public health

Published Papers (1 paper)

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Research

14 pages, 1134 KiB  
Article
Development of Polymerase Chain Reaction–High-Resolution Melt Assay for Waterborne Pathogens Legionella pneumophila, Vibrio parahaemolyticus, and Camplobacter jejuni
by Shannon M. Carr and Kelly M. Elkins
Microorganisms 2024, 12(7), 1366; https://doi.org/10.3390/microorganisms12071366 - 3 Jul 2024
Viewed by 325
Abstract
Legionella pneumophila is the waterborne pathogen primarily responsible for causing both Pontiac Fever and Legionnaire’s Disease in humans. L. pneumophila is transmitted via aerosolized water droplets. The purpose of this study was to design and test primers to allow for rapid polymerase chain [...] Read more.
Legionella pneumophila is the waterborne pathogen primarily responsible for causing both Pontiac Fever and Legionnaire’s Disease in humans. L. pneumophila is transmitted via aerosolized water droplets. The purpose of this study was to design and test primers to allow for rapid polymerase chain reaction (PCR) melt detection and identification of this infectious agent in cases of clinical or emergency response detection. New PCR primers were designed for this species of bacteria; the primer set was purchased from IDT and the target bacterial DNA was purchased from ATCC. The L. pneumophila primers targeted the macrophage infectivity potentiator gene (mip), which inhibits macrophage phagocytosis. The primers were tested for specificity, repeatability, and sensitivity using PCR–high-resolution melt (HRM) assays. The primer set was found to be specific to the designated bacteria and did not amplify the other twenty-one species from the panel. The L. pneumophila assay was able to be multiplexed. The duplex assay consists of primers for L. pneumophila and Vibrio parahaemolyticus, which are both waterborne pathogens. The triplex assay consists of primers for L. pneumophila, V. parahaemolyticus, and Campylobacter jejuni. The unique melting temperature for the L. pneumophila primer assay is 82.84 ± 0.19 °C, the C. jejuni assay is 78.10 ± 0.58 °C, and the V. parahaemolyticus assay is 86.74 ± 0.65 °C. Full article
(This article belongs to the Special Issue Advances in Research on Waterborne Pathogens)
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