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Biomanufacturing of Natural Bioactive Compounds
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Biomanufacturing of Natural Bioactive Compounds

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Natural Products Chemistry".

Deadline for manuscript submissions: 31 December 2024 | Viewed by 4056

Special Issue Editor

Dr. Honglei Liu

E-Mail Website
Guest Editor
State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China
Interests: bioactive compounds; sulfur metabolism; biosynthesis; podophylotoxin

Special Issue Information

Dear Colleagues,

Natural bioactive compounds are natural products with pharmacological activity produced by animal, plants, and microorganisms, such as podophyllotoxin, paclitaxel, and (-)-β-elemene. These compounds are commonly secondary metabolic products with complex structures and low yields, which make it difficult to obtain them as well as synthesize them chemically.

Biosynthesis, with the use enzymatic reactions, shows prominent advantage in the synthesis of natural bioactive products. Using cell factory or engineering enzyme to synthesize and catalyze the production of highly active natural products has broad prospects. The cellular uptake of special substrates, biosynthesis using enzymatic reactions with cloned gene (clusters) to improve yields or activity, and improved final products extraction methods are widely studied by many groups.

This Special Issue aims to provide a broad overview of the biosynthesis of natural bioactive compounds. Original research articles or reviews that discuss the transport of complicated substrates, expression of related genes, biosynthesis from simple substrate or derivation from prodrugs, and improved extraction methodologies of natural bioactive compounds as well as other related articles are welcome.

Dr. Honglei Liu
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at mdpi.longhoe.net by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bioactive compounds
  • natural products
  • biosynthesis
  • derivation
  • cell factory

Published Papers (4 papers)

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Research

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23 pages, 1786 KiB  
Article
UHPLC-HRMS/MS Chemical Fingerprinting of the Bioactive Partition from Cultivated Piper aduncum L.
by Adélia Viviane de Luna, Thayssa da Silva Ferreira Fagundes, Ygor Jessé Ramos, Marlon Heggdorne de Araújo, Michelle Frazão Muzitano, Sanderson Dias Calixto, Thatiana Lopes Biá Ventura Simão, George Azevedo de Queiroz, Elsie Franklin Guimarães, André Mesquita Marques and Davyson de Lima Moreira
Molecules 2024, 29(8), 1690; https://doi.org/10.3390/molecules29081690 - 9 Apr 2024
Viewed by 1289
Abstract
Piper aduncum L. is widely distributed in tropical regions and the ethnobotanical uses of this species encompass medicinal applications for the treatment of respiratory, antimicrobial, and gynecological diseases. Chemical studies reveal a diverse array of secondary metabolites, including terpenes, flavonoids, and prenylated compounds. [...] Read more.
Piper aduncum L. is widely distributed in tropical regions and the ethnobotanical uses of this species encompass medicinal applications for the treatment of respiratory, antimicrobial, and gynecological diseases. Chemical studies reveal a diverse array of secondary metabolites, including terpenes, flavonoids, and prenylated compounds. Extracts from P. aduncum have shown antibacterial, antifungal, and larvicidal activities. Our study explores the activity of extracts and partitions against Mycobacterium tuberculosis H37Rv, as well as the chemical diversity of the bioactive partition. This marks the first investigation of the bioactive partition of P. aduncum from agroecological cultivation. The ethyl acetate partition from the ethanolic leaf extract (PAEPL) was found to be the most active. PAEPL was subjected to column chromatography using Sephadex LH-20 and the obtained fractions were analyzed using UHPLC-HRMS/MS. The MS/MS data from the fractions were submitted to the online GNPS platform for the generation of the molecular network, which displayed 1714 nodes and 167 clusters. Compounds were identified via manual inspection and different libraries, allowing the annotation of 83 compounds, including flavonoids, benzoic acid derivatives, glycosides, free fatty acids, and glycerol-esterified fatty acids. This study provides the first chemical fingerprint of an antimycobacterial sample from P. aduncum cultivated in an agroecological system. Full article
(This article belongs to the Special Issue Biomanufacturing of Natural Bioactive Compounds)
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Review

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15 pages, 2183 KiB  
Review
Natural Bioactive Compounds in the Management of Periodontal Diseases: A Comprehensive Review
by Nada Tawfig Hashim, Rasha Babiker, Muhammed Mustahsen Rahman, Riham Mohamed, Sivan Padma Priya, Nallan CSK Chaitanya, Md Sofiqul Islam and Bakri Gobara
Molecules 2024, 29(13), 3044; https://doi.org/10.3390/molecules29133044 - 26 Jun 2024
Viewed by 793
Abstract
Periodontal diseases, chronic inflammatory conditions affecting oral health, are primarily driven by microbial plaque biofilm and the body’s inflammatory response, leading to tissue damage and potential tooth loss. These diseases have significant physical, psychological, social, and economic impacts, necessitating effective management strategies that [...] Read more.
Periodontal diseases, chronic inflammatory conditions affecting oral health, are primarily driven by microbial plaque biofilm and the body’s inflammatory response, leading to tissue damage and potential tooth loss. These diseases have significant physical, psychological, social, and economic impacts, necessitating effective management strategies that include early diagnosis, comprehensive treatment, and innovative therapeutic approaches. Recent advancements in biomanufacturing have facilitated the development of natural bioactive compounds, such as polyphenols, terpenoids, alkaloids, saponins, and peptides, which exhibit antimicrobial, anti-inflammatory, and tissue regenerative properties. This review explores the biomanufacturing processes—microbial fermentation, plant cell cultures, and enzymatic synthesis—and their roles in producing these bioactive compounds for managing periodontal diseases. The integration of these natural compounds into periodontal therapy offers promising alternatives to traditional treatments, potentially overcoming issues like antibiotic resistance and the disruption of the natural microbiota, thereby improving patient outcomes. Full article
(This article belongs to the Special Issue Biomanufacturing of Natural Bioactive Compounds)
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44 pages, 3084 KiB  
Review
Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate–Oxaloacetate–Pyruvate-Derived Amino Acids
by Lianghong Yin, Yanan Zhou, Nana Ding and Yu Fang
Molecules 2024, 29(12), 2893; https://doi.org/10.3390/molecules29122893 - 18 Jun 2024
Viewed by 336
Abstract
The phosphoenol pyruvate–oxaloacetate–pyruvate-derived amino acids (POP-AAs) comprise native intermediates in cellular metabolism, within which the phosphoenol pyruvate–oxaloacetate–pyruvate (POP) node is the switch point among the major metabolic pathways existing in most living organisms. POP-AAs have widespread applications in the nutrition, food, and pharmaceutical [...] Read more.
The phosphoenol pyruvate–oxaloacetate–pyruvate-derived amino acids (POP-AAs) comprise native intermediates in cellular metabolism, within which the phosphoenol pyruvate–oxaloacetate–pyruvate (POP) node is the switch point among the major metabolic pathways existing in most living organisms. POP-AAs have widespread applications in the nutrition, food, and pharmaceutical industries. These amino acids have been predominantly produced in Escherichia coli and Corynebacterium glutamicum through microbial fermentation. With the rapid increase in market requirements, along with the global food shortage situation, the industrial production capacity of these two bacteria has encountered two bottlenecks: low product conversion efficiency and high cost of raw materials. Aiming to push forward the update and upgrade of engineered strains with higher yield and productivity, this paper presents a comprehensive summarization of the fundamental strategy of metabolic engineering techniques around phosphoenol pyruvate–oxaloacetate–pyruvate node for POP-AA production, including L-tryptophan, L-tyrosine, L-phenylalanine, L-valine, L-lysine, L-threonine, and L-isoleucine. Novel heterologous routes and regulation methods regarding the carbon flux redistribution in the POP node and the formation of amino acids should be taken into consideration to improve POP-AA production to approach maximum theoretical values. Furthermore, an outlook for future strategies of low-cost feedstock and energy utilization for develo** amino acid overproducers is proposed. Full article
(This article belongs to the Special Issue Biomanufacturing of Natural Bioactive Compounds)
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35 pages, 19299 KiB  
Review
Strategies, Achievements, and Potential Challenges of Plant and Microbial Chassis in the Biosynthesis of Plant Secondary Metabolites
by Taotao Han and Guopeng Miao
Molecules 2024, 29(9), 2106; https://doi.org/10.3390/molecules29092106 - 2 May 2024
Viewed by 1109
Abstract
Diverse secondary metabolites in plants, with their rich biological activities, have long been important sources for human medicine, food additives, pesticides, etc. However, the large-scale cultivation of host plants consumes land resources and is susceptible to pest and disease problems. Additionally, the multi-step [...] Read more.
Diverse secondary metabolites in plants, with their rich biological activities, have long been important sources for human medicine, food additives, pesticides, etc. However, the large-scale cultivation of host plants consumes land resources and is susceptible to pest and disease problems. Additionally, the multi-step and demanding nature of chemical synthesis adds to production costs, limiting their widespread application. In vitro cultivation and the metabolic engineering of plants have significantly enhanced the synthesis of secondary metabolites with successful industrial production cases. As synthetic biology advances, more research is focusing on heterologous synthesis using microorganisms. This review provides a comprehensive comparison between these two chassis, evaluating their performance in the synthesis of various types of secondary metabolites from the perspectives of yield and strategies. It also discusses the challenges they face and offers insights into future efforts and directions. Full article
(This article belongs to the Special Issue Biomanufacturing of Natural Bioactive Compounds)
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